Selective and sensitive electrochemical biosensing of superoxide anion production by biological systems : a short overview of recent trends

Pontié, Maxime; Bedioui, Féthi

doi:10.1051/analusis:1999270564

Cited by 9 publications

(8 citation statements)

References 36 publications

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“…Probably this behavior was due to the presence of some substances that caused the biosensor surface to become dirty and cloudy. This problem is mainly associated to protein fouling and subsequent loss of electrochemical response [26,27]. Analogous behavior was observed by us using other working electrodes.…”

Section: In Vivo Measurementssupporting

confidence: 77%

Amperometric Glucose Determination by Means of Glucose Oxidase Immobilized on a Cellulose Acetate Film: Dependence on the Immobilization Procedures

et al. 2007

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Glucose microelectrodes were prepared by immobilizing glucose oxidase onto a cellulose acetate film coating a platinum wire. Hexamethylenediamine (HMDA) and Glutaraldehyde (GA) were employed as spacer and coupling agent, respectively. Sensitivities and linear response ranges were studied as a function of the relative amounts of HMDA and GA. The best sensitivity was found when HMDA and GA were 5% and 2.5% in aqueous solutions, respectively. Taking as a reference the functioning of this biosensor, the roles of HMDA and GA percentages appear to be opposed when the extension of the linear response range is considered. Indeed, an increase of one unit in HMDA percentage (from 5 to 6 %) induces an increase in the extension of the linear response range equal to that obtained with a decrease of one unit of GA percentage (from 2.5 to 1.5%). The functioning of the biosensor displaying the highest sensitivity was tested with pineapple juice and rat serum, giving results comparable to those obtained by means of the glu-cinet test. Moreover, the in vivo glucose measurement during induced glycaemia in Sprague Dawley rats by intraperitoneal glucose injections resulted similar to that obtained by using a commercial glucometer.

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Section: In Vivo Measurementssupporting

confidence: 77%

Amperometric Glucose Determination by Means of Glucose Oxidase Immobilized on a Cellulose Acetate Film: Dependence on the Immobilization Procedures

et al. 2007

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“…Ag/AgCl to estimate the H 2 O 2 produced by the enzyme disproportionation of O 2 − through its oxidation current. Similarly, this biosensor uses a subtraction method to eliminate the current generated due to the natural disproportionation of O 2 − measured using a second electrode which consisted of bovine serum albumin (BSA) coated PACE …”

Section: Resultsmentioning

confidence: 99%

In-VitroDevelopment and Characterisation of a Superoxide Dismutase-Based Biosensor.

2017

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A first generation amperometric biosensor for the detection of superoxide (O 2 À ) was constructed utilising a dip-coating approach for immobilising the enzyme superoxide dismutase (SOD, 200 U/mL) onto a Pt electrode. Several dip-coating procedures were investigated, incorporating styrene, glutaraldehyde, bovine serum albumin and polyethylenimine at various concentrations, in order to develop a simple and reproducible coating method to maximise the sensitivity of the sensor to O 2 À . The optimised design was produced using 5 dip-coatings and a composite containing 200 U/mL SOD, 0.5 % glutaralde-hyde and 2 % polyethylenimine (Sty-(SOD-0.5 %GA-2 %PEI) 5 ). This sensor displayed excellent permselective characteristics with negligible signals produced by 12 of the most common electroactive species present in brain extracellular fluid, including uric acid (UA) which is produced as a by-product of the O 2 À generating xanthine-xanthine oxidase calibration method. In addition, it had a response time of ca. 1 s, high sensitivity to O 2 À (0.91 AE 0.02 nA/mM), and an in-vitro limit of detection of ca.0.063 mM, thus suggesting potential use for neurochemical monitoring of O 2 À in-vivo.

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“…The description of some signi®cant designs of O À 2 -electrochemical sensors has been recently reviewed [2]. An especially fruitful strategy for reaching highly speci®c and selective electrochemical sensing of O À 2 is the use of protein or enzyme-based biosensors, namely cytochrome c or SOD.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Selectivity of Overoxidized Polypyrrole/Superoxide Dismutase Based Microsensor for the Electrochemical Measurement of Superoxide Anion in Solution

Descroix

Bedioui

2001

Electroanalysis

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We have analyzed in this study the selectivity of an overoxidized polypyrrole/superoxide dismutase (SOD) coated platinum electrode towards the biosensing of superoxide anion in phosphate buffer solution (pH 7.4). We have dismanteled the different steps involved in the construction of this biosensor to provide a technical examination of its way of working. Our investigation showed that if the overoxidized polypyrrole coating is not permeable to hydrogen peroxide from the bulk solution, no amperometric signal due to the oxidation of hydrogen peroxide formed within the coating by catalyzed disproportionation of O2− by SOD is observed. This may be explained in terms of anion‐exclusion behavior of the overoxidized polypyrrole coating.

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Selective and sensitive electrochemical biosensing of superoxide anion production by biological systems : a short overview of recent trends

Cited by 9 publications

References 36 publications

Amperometric Glucose Determination by Means of Glucose Oxidase Immobilized on a Cellulose Acetate Film: Dependence on the Immobilization Procedures

Amperometric Glucose Determination by Means of Glucose Oxidase Immobilized on a Cellulose Acetate Film: Dependence on the Immobilization Procedures

In-VitroDevelopment and Characterisation of a Superoxide Dismutase-Based Biosensor.

Evaluation of the Selectivity of Overoxidized Polypyrrole/Superoxide Dismutase Based Microsensor for the Electrochemical Measurement of Superoxide Anion in Solution

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