Selective and Sensitive Detection of Heavy Metal Ions in 100% Aqueous Solution and Cells with a Fluorescence Chemosensor Based on Peptide Using Aggregation-Induced Emission

Abstract

A fluorescent peptidyl chemosensor for the detection of heavy metal ions in aqueous solution as well as in cells was synthesized on the basis of the peptide receptor for the metal ions using an aggregation-induced emission fluorophore. The peptidyl chemosensor (1) bearing tetraphenylethylene fluorophore showed an exclusively selective turn-on response to Hg(2+) among 16 metal ions in aqueous buffered solution containing NaCl. The peptidyl chemosensor complexed Hg(2+) ions and then aggregated in aqueous buffered solution, resulting in the significant enhancement (OFF-On) of emissions at around 470 nm. The fluorescent sensor showed a highly sensitive response to Hg(2+), and about 1.0 equiv of Hg(2+) was enough for the saturation of the emission intensity change. The detection limit (5.3 nM, R(2) = 0.99) of 1 for Hg(2+) ions was lower than the maximum allowable level of Hg(2+) in drinking water by EPA. Moreover, the peptidyl chemosensor penetrated live cells and detected intracellular Hg(2+) ions by the turn-on response.

Optimization of Glutaraldehyde Vapor Treatment for Electrospun Collagen/Silk Tissue Engineering Scaffolds

“…It was also evidenced by the increased fiber fluorescence, which is attributed to the formation of the Schiff base as well as the increased fiber stiffness that restricts intramolecular rotation of the fluorophores, leading to the enhancement of emission. 37 Native collagen is made up of strands of triple α helices. The FTIR spectroscopic analysis suggests that the α helix is the dominant structure in as-spun collagen fibers, consistent with the GXY repeating sequences in collagen peptides.…”

Facile synthesis of AgNPs on reduced graphene oxide for highly sensitive simultaneous detection of heavy metal ions

“…[1][2][3][4][5][6] Therefore, it is signicant to develop direct and highly sensitive tools to determine the concentration of heavy metals in solutions. To date, many kinds of techniques have been applied for the detection of heavy metal ions, including mass spectrometry, 7 optical methods 8 and electrochemical methods. 9 Recent developments in materials science and technology have enabled rapid electrochemical analysis with low power consumption, high sensitivity and wide adaptability, 10 and therefore electrochemical analysis methods for detecting heavy metals certainly have great potential.…”

A reusable thioether-rich crown-based fluorescent sensor for the detection and removal of mercuric ions

“…Upon addition of Hg 2+ , FS-G showed an overall emission change of 5-fold with a very good linearity (correlation coefficient of R 2 = 0.9992) in the range 0-10 μM of Hg 2+ with a detection limit of 33.4 ppb. On the other hand, FS-G shows outstanding ability of adsorbing and removing Hg 2+ from water which is not possessed by other fluorescent probes towards Hg 2+ [37][38][39], and the maximum adsorption capacity was 7.4 mg g -1 . By using Dimercaptosuccinic Acid (DMSA), FS-G can be regenerated and reused for at least five times, which shows the potential applications for the detection and removal of Hg 2+ in real biological and environmental areas.…”