Selective and programmed cleavage of GPI-anchored proteins from the surface membrane by phospholipase C

Müller, Alexandra; Klöppel, Christine; Smith-Valentine, Megan; Houten, Judith Van; Simón, Martín

doi:10.1016/j.bbamem.2011.10.009

Cited by 35 publications

(24 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ankyrin domain is considered to be the most common location for protein–protein interactions [47] and can play a role in several cellular functions. TisoTranscripts-59 was identified as a GPI protein-like, a category of proteins known for their roles in communication between cells [48]. Given their putative functions, a potential implication of these latter two genes in the lipid over-accumulation observed in Tiso-S2M2 is not obvious, but they should be kept in mind for further studies.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome of Wild Type and Selected Strains of the Microalgae Tisochrysis lutea Provides Insights into the Genetic Basis, Lipid Metabolism and the Life Cycle

Carrier

Garnier

Cunff³

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

The applied exploitation of microalgae cultures has to date almost exclusively involved the use of wild type strains, deposited over decades in dedicated culture collections. Concomitantly, the concept of improving algae with selection programs for particular specific purposes is slowly emerging. Studying since a decade an economically and ecologically important haptophyte Tisochrysis lutea (Tiso), we took advantage of the availability of wild type (Tiso-Wt) and selected (Tiso-S2M2) strains to conduct a molecular variations study. This endeavour presented substantial challenges: the genome assembly was not yet available, the life cycle unknown and genetic diversity of Tiso-Wt poorly documented. This study brings the first molecular data in order to set up a selection strategy for that microalgae.Following high-throughput Illumina sequencing, transcriptomes of Tiso-Wt and Tiso-S2M2 were de novo assembled and annotated. Genetic diversity between both strains was analyzed and revealed a clear conservation, while a comparison of transcriptomes allowed identification of polymorphisms resulting from the selection program. Of 34,374 transcripts, 291 were differentially expressed and 165 contained positional polymorphisms (SNP, Indel). We focused on lipid over-accumulation of the Tiso-S2M2 strain and 8 candidate genes were identified by combining analysis of positional polymorphism, differential expression levels, selection signature and by study of putative gene function. Moreover, genetic analysis also suggests the existence of a sexual cycle and genetic recombination in Tisochrysis lutea.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome of Wild Type and Selected Strains of the Microalgae Tisochrysis lutea Provides Insights into the Genetic Basis, Lipid Metabolism and the Life Cycle

Carrier

Garnier

Cunff³

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…), only two appear GPI‐specific—a situation which is even less clear in mammals (Müller et al. ; Staudt et al. ).…”

Section: Specific Conservation and Changes During Evolutionmentioning

confidence: 99%

“…The GPI anchor of proteins can be released by specific phospholipase C (PL-C) forms. From bacterial precursors and from a small number of PL-C paralogs (Leondaritis et al 2011), only two appear GPI-specific-a situation which is even less clear in mammals (M€ uller et al 2012;Staudt et al 2016). GPI proteins accompany evolution of Mono-and Bikonta from low levels on.…”

Section: Gpi-anchored Variant Surface Antigens (Vsags)mentioning

confidence: 99%

Evolutionary Cell Biology of Proteins from Protists to Humans and Plants

Plattner

2017

J Eukaryotic Microbiology

View full text Add to dashboard Cite

During evolution, the cell as a fine-tuned machine had to undergo permanent adjustments to match changes in its environment, while "closed for repair work" was not possible. Evolution from protists (protozoa and unicellular algae) to multicellular organisms may have occurred in basically two lineages, Unikonta and Bikonta, culminating in mammals and angiosperms (flowering plants), respectively. Unicellular models for unikont evolution are myxamoebae (Dictyostelium) and increasingly also choanoflagellates, whereas for bikonts, ciliates are preferred models. Information accumulating from combined molecular database search and experimental verification allows new insights into evolutionary diversification and maintenance of genes/proteins from protozoa on, eventually with orthologs in bacteria. However, proteins have rarely been followed up systematically for maintenance or change of function or intracellular localization, acquirement of new domains, partial deletion (e.g. of subunits), and refunctionalization, etc. These aspects are discussed in this review, envisaging "evolutionary cell biology." Protozoan heritage is found for most important cellular structures and functions up to humans and flowering plants. Examples discussed include refunctionalization of voltage-dependent Ca 2+

show abstract

“…Finally, a functionality unique to GPI-anchored proteins in lower and higher eukaryotes is that they can be released from the cell surface by cleavage of the lipid anchor by phosphatidylinositol-specific endogenous hydrolases (11) . Such phospholipases show specificity for specific GPI-APs (12) , for example, phospholipase C acts on acetylcholinesterase (13) and phospholipase D on alkaline phosphatase (14) . By controlling the release of GPI-APs from the membrane, these enzymes play a critical regulatory role in their expression and function (15) .…”

Section: Overview Of Protein Lipidationmentioning

confidence: 99%

Pharmacological Inhibition of Protein Lipidation

Ganesan¹,

Levental²

2015

J Membrane Biol

View full text Add to dashboard Cite

Lipid modifications of mammalian proteins are widespread, modifying thousands of targets centrally involved in cellular physiology. Broadly, these serve to increase protein hydrophobicity and association with cellular membranes. Often, these modifications are absolutely essential for protein stability and localization, and serve critical roles in dynamic regulation of protein function. A number of proteins affected by lipidation are associated with diseases, including parasite infections, neurological diseases, diabetes and cancer, suggesting that lipid modifications represent potentially attractive targets for pharmacological intervention. This review briefly describes the various types of post-translational protein lipid modifications, proteins modified by them, and the enzymatic machinery associated with these. We then discuss several case studies demonstrating successful development of lipidation inhibitors of potential (and more rarely, realized) clinical value. Although this field remains in its infancy, we believe these examples demonstrate the potential utility of targeting protein lipidation as a viable strategy for inhibiting the function of pathogenic proteins.

show abstract

Selective and programmed cleavage of GPI-anchored proteins from the surface membrane by phospholipase C

Cited by 35 publications

References 41 publications

Comparative Transcriptome of Wild Type and Selected Strains of the Microalgae Tisochrysis lutea Provides Insights into the Genetic Basis, Lipid Metabolism and the Life Cycle

Comparative Transcriptome of Wild Type and Selected Strains of the Microalgae Tisochrysis lutea Provides Insights into the Genetic Basis, Lipid Metabolism and the Life Cycle

Evolutionary Cell Biology of Proteins from Protists to Humans and Plants

Pharmacological Inhibition of Protein Lipidation

Contact Info

Product

Resources

About