Selective and fast methylation of free fatty acids directly in plasma for their individual analysis by gas chromatography- mass spectrometry

Ciucanu, Cristian I; Vlad, Daliborca; Ciucanu, Ionel; Dumitraşcu, Victor

doi:10.1016/j.chroma.2020.461259

Cited by 10 publications

(5 citation statements)

References 26 publications

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“…It is ultimately responsible for the hydrophobization effect achieved. Although DMSO is less basic with respect to carboxylic acids than water [47], its presence provides a sufficient solubility of stearic acid. Moreover, it may be expected that DMSO participates in the proton transfer from stearic acid to the metal surface.…”

Section: Resultsmentioning

confidence: 99%

Regularities of the Formation of a Green Superhydrophobic Protective Coating on an Aluminum Alloy after Surface Modification with Stearic Acid Solutions

Abrashov

Grigoryan

Korshak

et al. 2021

Metals

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It has been shown that solutions of stearic acid in a dimethyl sulfoxide–water binary mixture allow superhydrophobic protective coatings to be created on an aluminum alloy surface with a minimum impact on the environment. The superhydrophobicity and self-cleaning ability of the coating that we developed have been confirmed by measurements of droplet wetting angles and roll-off angles. These properties appear due to the formation of a multimodal micro-rough surface that mainly consists of aluminum stearate. The coatings formed in this manner have been studied by ellipsometry, XPS, and scanning probe microscopy. Their protective ability has been estimated by the “droplet-express” method and in a salt fog chamber. The protective ability of the coating is determined by the DMSO/H2O ratio, the concentration of stearic acid, and the duration and temperature of modification of the aluminum alloy; it is controlled by a competition between the processes of aluminum stearate formation and hydrolysis. It has been shown that adsorption of stearic acid on an aluminum stearate coating increases its permeability and decreases its protective capability. The results presented in this article are useful for optimizing the conditions of applying green superhydrophobic stearate coatings on aluminum alloys in order to achieve a maximum protective effect.

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Section: Resultsmentioning

confidence: 99%

Regularities of the Formation of a Green Superhydrophobic Protective Coating on an Aluminum Alloy after Surface Modification with Stearic Acid Solutions

Abrashov

Grigoryan

Korshak

et al. 2021

Metals

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“…All FAs from plasma lipids were methylated in 15 min at room temperature with methyl iodide in solution of dimethyl sulfoxide and by addition of solid sodium hydroxide [ 21 ]. FFAs were selectively methylated in 1 min at room temperature with methyl iodide in a solution of dimethyl sulfoxide and in the presence of anhydrous potassium carbonate [ 22 ]. FAs methyl esters were identified by mass spectrometry and on the basis of retention times of the FAs standards.…”

Section: Methodsmentioning

confidence: 99%

Effect of rosuvastatin on the concentration of each fatty acid in the fraction of free fatty acids and total lipids in human plasma: The role of cholesterol homeostasis

Ciucanu

Olariu

Vlad

et al. 2020

Biochemistry and Biophysics Reports

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Each fatty acid (FA) or class of FAs has a different behavior in the pathologies of atherosclerosis. The aim of this study was to investigate changes in the concentration of each fatty acid in the fraction of free fatty acids (FFAs) and total lipids in human plasma after short-term therapy with rosuvastatin as a cholesterol-lowering statin drug. Six hypercholesterolemic men on a habitual diet were studied in a randomized, double-blind, and crossover process. They received 20 mg rosuvastatin or placebo in random order, each for 4 weeks and after 2 weeks of washout period, they received another medication (placebo or rosuvastatin) for another period of 4 weeks. Rosuvastatin treatment significantly decreased the absolute concentrations of saturated and monounsaturated FAs in the total FAs as well as in FFAs. Long chain polyunsaturated fatty acids with 20 and 22 carbon atoms in the molecule had no significant change in the fraction of FFAs. Rosuvastatin is directly involved in cholesterol biosynthesis and indirectly through cholesterol homeostasis in the biosynthesis of other plasma lipids. In conclusion, our findings show that rosuvastatin treatment leads to significant changes in the concentration of each fatty acid, except for long-chain polyunsaturated fatty acids in FFAs. Our observations indicate that cholesterol homeostasis through its regulatory mechanisms appears to be the main cause of changes in the concentration of each plasma fatty acid during rosuvastatin treatment. These changes can be a source of beneficial consequences, in addition to lowering low-density lipoprotein cholesterol in cardiovascular diseases.

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“…Some of the main strategies include extraction methods (e.g., liquid-liquid extraction and solid-phase microextraction), derivatization methods, column selections, and internal standard selections, in order to identify and measure the concentration of various fatty acids in biomatrices. A recent GC-MS analytical procedure for the rapid and selective derivatization of free fatty acids into methyl esters directly in plasma without transmethylation of lipid-bound fatty acids was developed based on the reaction with CH 3 I in dimethyl sulfoxide and in the presence of solid bases (sodium carbonate) [48]. The method requires a very small volume of plasma (50 μL) and has a detection limit of 0.1 ng/mL.…”

Section: Gc Analysis Of Biotics In Biological Fluidsmentioning

confidence: 99%

Recent Applications of Gas Chromatography in Bioanalysis

David¹,

Moldoveanu²

2023

Novel Aspects of Gas Chromatography and Chemometrics

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Bioanalysis involves a broad range of chemical analyzes. These analyzes include that of biotics, such as natural components of living organisms, as well as xenobiotics, such as drugs and their metabolites in biological systems. Because many biotics and xenobiotics are not volatile molecules, the main technique for bioanalysis is high-performance liquid chromatography (HPLC) and the limitation of GC utilization is caused by the fact that GC is applicable only to volatile samples. However, gas chromatography (GC) in particular coupled with mass spectrometry (MS) as detection is also a very useful technique in bioanalysis. A considerable number of analytes in bioanalysis are volatile or can be made volatile following, for example, derivatization. As a result, GC (and GC/MS) are commonly utilized for the analysis of biotics, such as amino acids, fatty acids, various metabolites in biological fluids, and in particular of a large number of xenobiotics, such as drugs, drug metabolites, toxicants, and certain metabolic compounds caused by toxicants. The chapter will present progress in the GC methodology for extending its applicability to bioanalysis and will provide a review of more recent applications.

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Selective and fast methylation of free fatty acids directly in plasma for their individual analysis by gas chromatography- mass spectrometry

Cited by 10 publications

References 26 publications

Regularities of the Formation of a Green Superhydrophobic Protective Coating on an Aluminum Alloy after Surface Modification with Stearic Acid Solutions

Regularities of the Formation of a Green Superhydrophobic Protective Coating on an Aluminum Alloy after Surface Modification with Stearic Acid Solutions

Effect of rosuvastatin on the concentration of each fatty acid in the fraction of free fatty acids and total lipids in human plasma: The role of cholesterol homeostasis

Recent Applications of Gas Chromatography in Bioanalysis

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