Selective adsorption of oligonucleotides on switchable self-assembled monolayers

Sellergren, Börje; Auer, Frank; Arnebrant, Thomas

doi:10.1039/a905193g

Cited by 19 publications

(21 citation statements)

References 12 publications

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“…Treatment with various functionalized silanes, such as trialkoxy-3-aminopropylsilane 113,114,143,200,219,227,286,388,395) , provides an additional, "easy" access to positively charged surfaces. In a similar way, treatment of gold with functionalized thiols or disulfides, respectively, enables the tailoring of both positively (e. g. when applying cysteamine 119,211,277,296,387) ) or negatively (e. g. when applying mercaptoundecanoic acid 115,116,228,269,286,396,397) ) charged surfaces. As discussed above, film growth is characteristic for a chosen pair of polyelectrolytes and its "matching", and on the conditions for adsorption.…”

Section: Useful Substratesmentioning

confidence: 98%

“…Also, much interest has been given to the conversion of substrates by enzymes in ESA-films 248,256,340,[346][347][348][349] . Molecular recognition reactions were studied, too 116,149,248,337,338,350,358) . Whereas low molar mass compounds seem to be able to permeate deeply into the films, the reaction of large molecules such as proteins or DNA fragments seems to be limited to the surface 350,452) .…”

Section: Chemical Reactions In Films Made By Esamentioning

confidence: 99%

“…The investigations were mostly motivated by their biological function and biomimicking applications, e. g. for biocompatibilization of surfaces, enzymatic activity, sensing purposes, or molecular recognition. Ribonucleic and desoxyribonucleic acids (RNA and DNA) are strong polyanions due to the phosphate esters in their backbone and thus deposit easily, as do the model homopolynucleotides 24,26,36,38,116,317,[331][332][333][334][335][336][337][338][339][340][341][342][343] . Whereas intercalation studies have been successful [333][334][335]341) , molecular recognition and selective pairing of single strands is difficult to accomplish yet in ESA-films 26,116,333,338) .…”

Section: Polymers Suited For Esamentioning

confidence: 99%

“…Ribonucleic and desoxyribonucleic acids (RNA and DNA) are strong polyanions due to the phosphate esters in their backbone and thus deposit easily, as do the model homopolynucleotides 24,26,36,38,116,317,[331][332][333][334][335][336][337][338][339][340][341][342][343] . Whereas intercalation studies have been successful [333][334][335]341) , molecular recognition and selective pairing of single strands is difficult to accomplish yet in ESA-films 26,116,333,338) . Also, a number of mixed protein-polyelectrolyte or protein-protein films 38,108,109,155,202,209,237,248,253,256,265,274,277,279,317,318,[344][345][346][347]…”

Section: Polymers Suited For Esamentioning

confidence: 99%

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Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Bertrand

Jonas

Laschewsky³

et al. 2000

Macromol. Rapid Commun.

1,169

1,184

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A bit of historyThe key role of surfaces for many material properties as well as for many biological processes has been recognized now. Therefore, new strategies aim at the tailoring of the material's surface only -or of a thin surface layer, respectively -, while preserving the bulk properties of the underlying support. Particular emphasis has been given to the surface modification by polymers, in an attempt to extend the known versatility of polymer bulk materials to ultrathin films and coatings, and to prepare bulk-surface composite materials. The self-organization of polymers has been increasingly explored for the preparation of well-defined surfaces and interfaces in recent years, extending the use of the established methods of low molar mass compounds (for comparative reviews, see ref. 1-3)). With such techniques, polymer films are formed spontaneously on substrates, due to balanced interactions between substrate, polymer (or its precursor) and medium. Typically, very thin, often monomolecular layers are produced. Repetitive deposition steps provide a precise control over the total thickness of the coatings, in the range from a few angstroms up to the micrometer range. Moreover, the step-by-step procedures allow for a fine structuring in the third dimension. In addition to the preparation of uniform and homogeneous coatings, gratings, gradients or steps of defined height in molecular dimensions can be easily constructed.The most recent of the self-organization techniques is the alternating physisorption of oppositely charged polyelectrolytes, the so-called "layer-by-layer" method or "electrostatic self-assembly" (ESA) [3][4][5][6][7][8][9][10] . Although some early, singular studies of self-assembly by alternating adsorption of oppositely charged polyions are reported [11][12][13] , a practical method for ESA was developed Feature Article: The article presents the state-of-the-art of alternating physisorption of oppositely charged polyelectrolytes, the so-called "layer-by-layer" method or "electrostatic self-assembly" (ESA), for the preparation of thin polymer coatings. In comparison to other, more established self-organization techniques, this recent method is distinguished by its simplicity, versatility, and speed. In particular, the tendency for self-healing is unique. Emphasis is given to the role of the molecular structure of the polyelectrolytes, and to the nature of the support. Also, various parameters for the preparation of multilayer films are highlighted, which are very important due to the kinetic control of the build-up process. The structure of the resulting coatings, their quality and stability, chemical reactions in the films, and potential applications are discussed.Macromol. Rapid Commun. 21, No. 7

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Section: Useful Substratesmentioning

confidence: 98%

Section: Chemical Reactions In Films Made By Esamentioning

confidence: 99%

Section: Polymers Suited For Esamentioning

confidence: 99%

Section: Polymers Suited For Esamentioning

confidence: 99%

See 2 more Smart Citations

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Bertrand

Jonas

Laschewsky³

et al. 2000

Macromol. Rapid Commun.

1,169

1,184

View full text Add to dashboard Cite

show abstract

“…Conditions could be found that allow repeated assembly–test–disassembly cycles of the layer structure. This restorable substrate concept was demonstrated for the detection of single mismatches in DNA hybridization experiments10 for selective detection of phosphate‐containing biomolecules,11 for the charge‐selective adsorption of plasma proteins,9 and for the rapid formation of dense layers of gold nanoparticles 12. After the “sensing” or adsorption event, the layers could be destabilized and the substrate restored.…”

Section: Introductionmentioning

confidence: 99%

Odd–Even Chain Length‐Dependent Order in pH‐Switchable Self‐Assembled Layers

Auer¹,

Nelles

Sellergren

2004

Chemistry A European J

Self Cite

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The reversible self-assembly of a series of bipolar amphiphiles, alpha,omega-bis(3- or 4-amidinophenoxy)alkanes (chain length n = 5-12), on mercaptoalkanoic acid-functionalized gold surfaces (chain length n = 10, 11, 14, 15) has been studied by in-situ ellipsometry, IR reflection absorption spectroscopy (IRAS), and atomic force microscopy (AFM). The layer order, amphiphile orientation, and tendency to form bilayers depends on the position of the amidine substituent, the alkyl chain length of both the amidine amphiphile and the underlying acid self-assembled monolayer (SAM), and whether the amidine alkyl chain contained an even or odd number of methylene groups. Thus, para-substituted bisbenzamidines containing more than six methylene groups (n>6) and with an odd number (n = 7, 9, 11) tended to form bilayered structures, whereas those containing an even number formed monolayers when adsorbed on SAMs of the long-chain acids (n = 14, 15). This behavior also correlated with the average tilt angle of the benzene moieties of the amphiphiles, as estimated by IRAS. The odd-numbered chains gave lower tilt angles than the even-numbered ones, and a possible model that accounts for these results is proposed. IRAS also revealed a higher order of the odd-numbered chains and an increasing hydrogen-bonding contribution with increasing chain length. Additional evidence for the proposed bilayered assemblies and their reversibility was obtained by AFM. Images obtained from the assembly of decamidine on a SAM of mercaptohexadecanoic acid in a pH 9 borate buffer revealed domains of similar size to that of the underlying acid SAM (20-30 nm), but less densely packed. By acidifying the solution, the second layer was destabilized and a very smooth layer with few defects appeared. Further acidification to pH 3 also destabilized the first layer.

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Self‐Assembled Monolayers with Dynamicity Stemming from (Bio)Chemical Conversions: From Construction to Application

Choi

Yeo

2012

ChemPhysChem

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Surfaces with "dynamicity" whereby surface properties can be modulated by an external stimulus on user demand have been actively exploited for the past decade. These switchable surfaces with dynamic properties are widely used for a number of applications such as micro/nanoarrays, biomolecule immobilization, basic cell studies, and tissue engineering on a variety of materials. This minireview highlights the dynamic control of surface properties on self-assembled monolayers and focuses on dynamicity that stems from (bio)chemical conversions achieved by electrical potentials, photoillumination, chemical reagents, enzymes, and pH.

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Selective adsorption of oligonucleotides on switchable self-assembled monolayers

Abstract: Short oligonucleotides adsorb rapidly and selectively on pHswitchable self-assembled layers of bisbenzamidines on modified gold substrates.

Cited by 19 publications

References 12 publications

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Odd–Even Chain Length‐Dependent Order in pH‐Switchable Self‐Assembled Layers

Self‐Assembled Monolayers with Dynamicity Stemming from (Bio)Chemical Conversions: From Construction to Application

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