Selection of turmeric callus tolerant to culture filtrate of Pythium Graminicolum and regeneration of plants

Gayatri, M. C.; darshini, V. Roopa; Kavyashree, R.

doi:10.1007/s11240-005-3706-0

Cited by 23 publications

(3 citation statements)

References 26 publications

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“…This has been shown to increase the frequency of solid mutants compared to in vivo radiation [41]. The number of selection cycles used and the duration of the selection pressure by exposure to filtrate in vitro, are essential factors for the isolation of tolerant material, since more than three cycles of continuous selection in vitro can give rise to undesirable alterations such as polyploidy, reduced regeneration capacity [49] and could induce secondary mutations [43].…”

Section: Discussionmentioning

confidence: 99%

Effect of Gamma Irradiation and Selection with Fungus Filtrate (Rhizoctonia solani Kuhn) on the in Vitro Culture of Common Bean (Phaseolus vulgaris)

Solís-Ramos¹,

Valdez-Melara²,

Alvarado-Barrantes³

et al. 2015

AJPS

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The present investigation was undertaken to study the effect of gamma irradiation (dose from 10 to 100 Gy) and in vitro selection with fungus filtrate as selecting agent (concentration from 20% to 100%) on the susceptibility of the common bean to Rhizoctonia solani. The best results were found with a dose of 20 Gy or a concentration of 20% of fungus filtrate applied separately. These conditions were used to evaluate the combined effect of both approaches in a second experiment. The combined effect of irradiation and then selection adversely affected growth (height and roots) and survival of the in vitro plants. It may not be necessary to combine the variation generated by irradiation with the selection technique. For future assays we propose the application of: 1) gamma radiation, thereby inducing not only mutants with pathogen resistance, but also with other agronomic traits of interest. Later in the subculture MV4 potential fungus-resistant mutants will be evaluated in the field; or 2) selection pressure using fungus filtrate during three subcultures, which may be sufficient to induce the variation necessary to obtain in vitro plants resistant to fungus.

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Section: Discussionmentioning

confidence: 99%

Effect of Gamma Irradiation and Selection with Fungus Filtrate (Rhizoctonia solani Kuhn) on the in Vitro Culture of Common Bean (Phaseolus vulgaris)

Solís-Ramos¹,

Valdez-Melara²,

Alvarado-Barrantes³

et al. 2015

AJPS

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“…For multiple shoot production, mostly vegetative bud explants (Salvi et al, 2002), sprouting buds from rhizomes (Balachandran et al, 1990), axillary buds from unsprouted rhizomes (Panda et al, 2007), pseudostem (Gayatri et al, 2005), leaf (Salvi et al, 2001), shoot tips (Salvi et al, 2001), immature inflorescence (Salvi et al, 2000), and nodal explants (Roy and Ray Chaudhuri, 2004) were used.…”

Section: Explants and Mediamentioning

confidence: 99%

The Biotechnology of Turmeric

Nair¹

2013

The Agronomy and Economy of Turmeric and Ginger

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Biotechnological interventions in turmeric can be summarized in the following sections. Tissue CultureTrue to type multiplication in turmeric is necessary for the following reasons: (i) low rhizome multiplication and difficulty in storage; (ii) limited availability of high-yielding genotypes; and (iii) expensive field maintenance and high susceptibility to pests and diseases. Tissue culture is one of the most important techniques to meet some of the above requirements. Tissue culture enables large-scale propagation in turmeric. Research in tissue culture is quite exhaustive. A number of researchers from all over the world have reported very effective in vitro culture protocols for direct and indirect regeneration from turmeric plant. However, only limited investigations refer to genetic fidelity of micropropagated plantlets using molecular tools.

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“…Root rot disease tolerant clones of turmeric cv. Suguna were isolated using continuous in vitro selection technique against pure culture filtrate of Pythium graminicolum (Gayatri et al, 2005).…”

Section: Somaclonal Variationmentioning

confidence: 99%

Spices biotechnology

Peter,

Babu,

2006

J. Hortic. Sci.

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In recent times, biotechnological tools have supplemented various conventional approaches in conservation,characterization, improvement and utilization for increasing production and productivity of spices. In manyspices, viable micropropagation technologies are available for commercial production and generation of disease - free planting material. Somaclonal variation is important in crops where natural variability is low and a few useful somaclonal variants have been identified in ginger, turmeric and vanilla. Protoplast technology is also available for capsicum, black pepper, fennel, fenugreek, garlic, saffron and peppermint. In vitro cryopreservation, Synseed and Micro-rhizome technologies are available for safe propagation, conservation, movement, and exchange of spices germplasm. Studies are in progress for in vitro production of flavour and colouring compounds like capsaicin, vanillin, anethole, crocin, picrocrocin, saff'ranal, etc. using immobilized and transformed cell cultures. Use of molecular markers for crop profiling, fingerprinting, molecular taxonomy, identification of duplicate hybrids, estimation of genetic fidelity and tagging of genes for marker aided selection (MAS) is gaining importance. Isolation of important and useful genes and development of transgenics is in the preliminary stage.

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Selection of turmeric callus tolerant to culture filtrate of Pythium Graminicolum and regeneration of plants

Cited by 23 publications

References 26 publications

Effect of Gamma Irradiation and Selection with Fungus Filtrate (Rhizoctonia solani Kuhn) on the in Vitro Culture of Common Bean (Phaseolus vulgaris)

Effect of Gamma Irradiation and Selection with Fungus Filtrate (Rhizoctonia solani Kuhn) on the in Vitro Culture of Common Bean (Phaseolus vulgaris)

The Biotechnology of Turmeric

Spices biotechnology

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