Selection of sugarcane plants resistant to SCMV

Zambrano, Asia Y.; Demey, Jhonny R.; Fuchs, Morela; González, Ventura; Rea, Ramón; Sousa, Orlando; Gutiérrez, Zulay

doi:10.1016/s0168-9452(03)00162-6

Cited by 37 publications

(10 citation statements)

References 17 publications

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“…Studies conducted in different crop plants suggest that salt tolerance is a multigenic complex trait (Niknam and McComb, 2000), which has seriously limited the efforts to develop the tolerant crop variety through conventional breeding practices. Somaclonal variation in combination with in vitro mutagenesis and selection has been applied for the isolation of agronomically useful mutants (Jain 2000;Zhambrano et al, 2003). Many examples related to different vegetatively propagated species, show that the combination of in vitro culture with selection is relatively inexpensive, simple and efficient (Ahloowalia, 1998).…”

Section: Introductionmentioning

confidence: 99%

An in vitro radiation induced mutagenesis-selection system for salinity tolerance in sugarcane

Patade

Suprasanna

2009

Sugar Tech

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In vitro radiation induced mutagenesis-selection was employed for salt tolerance in popular Indian sugarcane cv. Co 86032. Radiosensitivity characterized in terms of growth rate and cell viability indicated 20 Gy as LD 50 for embryogenic sugarcane callus cultures. Response of embryogenic calli exposed to different NaCl concentrations (85.6, 128.3, 171.1, 213.9 or 256.7 mM) indicated significant progressive decrease in cell viability and increase in membrane damage rate with increasing salt concentration. Based on these results, 20 Gy irradiated calli were stressed with 85.6 or 171.1 mM NaCl for selection for salt tolerance. Both irradiated or non-irradiated calli exhibited reduced growth rate compared to their respective controls, however, the extent of growth rate reduction was more in un-irradiated stressed calli. Further, in order to study the effect of auxin on salt selection, the calli were cultured on NaCl selection medium supplemented with or without auxin (2, 4-D). The growth rate was higher when NaCl media was supplemented with the auxin than that of without auxin. The calli exposed to 171.1 mM NaCl accumulated more proline than the control and 85.6 mM NaCl stressed irradiated or nonirradiated calli. Proline accumulation in the calli exposed to 85.6 mM NaCl without 2, 4-D was significantly higher than that of the calli stressed at the same salt concentration with 2, 4-D. The irradiated calli stressed with 85.6 mM NaCl medium with or without 2, 4-D accumulated more proline than the nonirradiated calli exposed to the respective salt treatments. Thus, the results suggest in vitro mutagenesis-selection as a powerful tool for enhanced tolerance to abiotic stresses, which may improve the competitiveness of the popular sugarcane cultivars and their commercial cultivation in saline areas.

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Section: Introductionmentioning

confidence: 99%

An in vitro radiation induced mutagenesis-selection system for salinity tolerance in sugarcane

Patade

Suprasanna

2009

Sugar Tech

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“…A successful induction of mutants with resistance to bacterial blight disease has been reported in common beans (Phaseolus vulgaris L.) (Mohamed et al 1995) and rice (Oryza sativa L.) (Agrawal et al 2005), using ionizing radiation. Gamma radiation has been successfully used to induce mutant varieties resistant to mosaic virus in sugarcane (Zambrano et al 2003), and tolerant to the fungus Fusarium oxysporum f. sp. cubense (Bhagwat and Duncan 1998) in banana, to heat (Das et al 2000) in potato, and to iron toxicity (Pathirana et al 2002) in rice.…”

Section: Introductionmentioning

confidence: 99%

Effects of ionizing radiation on the growth and allyl isothiocyanate accumulation of Wasabia japonica in vitro and ex vitro

Hung

Johnson

2007

In Vitro Cell.Dev.Biol.-Plant

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Mutations were induced in tissue-cultured wasabi (Wasabia japonica Matsumura) by treating in vitro-derived shoot tips with either γ-rays or X-rays at 0, 10, 20, 40 or 80 Gy. Doses of up to 40 Gy of either γ-or X-ray treatments resulted in a survival rate of more than 60% in culture after 3 mo. The use of γ-or X-rays at doses between 10 Gy and 40 Gy to induce mutation in W. japonica resulted in an alteration of the growth and allyl isothiocyanate (AITC) content of multiple shoots after 3 mo. in culture on Murashige and Skoog medium containing 5 μM N 6 -benzyladenine (BA). Putative mutants from the 40 Gy treatments of either γ-or X-rays exhibited a reduction in shoot weight, number, and height, whereas treatments of either γ-rays or X-rays at 10 Gy and 20 Gy doses showed no significant differences in shoot growth. All shoots treated with 80 Gy were either necrotic or irregenerable, while those treated with 40 Gy produced deformed leaves, from both types of ionizing radiation. Concentrations of AITC were measured by the use of gas chromatography-mass spectrometry (GC-MS). The accumulation of AITC was shown to decrease when doses increased in both γ-and Xray treatments, compared with the controls. Positive responses were solely occurred at 18 mo. after transfer of in vitro rooted shoots to the shade house. The survival rate, rhizome weight and AITC content of plants derived from shoots treated with 20 Gy or 40 Gy of either γ-rays or Xrays were significantly greater than those of the controls.

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“…In previous studies, RAPD markers were used for selecting sugarcane cultivars resistant or susceptible against various diseases, i.e. sugarcane mosaic virus (Zambrano et al 2003); red rot (Alvi et al 2008;Mumtaz et al 2011) andrust (Ali et al 2013;Muhammad et al 2013). AFLP, RFLP, SSR and ISSR markers were also identified against different diseases in sugarcane (McIntyre et al 2005b;Aljanabi et al 2007;Virupakshi and Naik 2008).…”

Section: Discussionmentioning

confidence: 99%

Red rot resistant gene characterization using RGAP markers among sugarcane cultivars resistant and susceptible to the red rot disease

Sharma

Tamta

2017

3 Biotech

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Sugarcane is the major source of sugar in Asia and Europe, grown primarily in the tropical and sub-tropical zones of the world. The main disease responsible for its low yield is red rot. Therefore, in the present study, characterization of red rot disease was performed among 55 different sugarcane cultivars varying in red rot resistance level. 18 fragments were found to be associated with red rot resistance and were identified as resistant specific markers. The resistant specific fragments were amplified by RGA169, RGA396, RGA129, RGA231, RGA251, RGA057, RGA118, RGA152, RGA327, RGA542, RGA012, RGA173, RGA184, RGA275, RGA019, RGA267, RGA281 and RGA533. 7 fragments were found to be associated with red rot susceptibility and were considered as susceptible specific markers amplified by RGA088, RGA162, RGA396, RGA231, RGA251, RGA087 and RGA275. Sequencing of five resistant fragments, viz., RGA169, RGA231, RGA251, RGA267 and RGA533 was performed and the data thus obtained showed 80-99% similarity when compared with other resistant gene sequences previously submitted in NCBI database.

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Selection of sugarcane plants resistant to SCMV

Cited by 37 publications

References 17 publications

An in vitro radiation induced mutagenesis-selection system for salinity tolerance in sugarcane

An in vitro radiation induced mutagenesis-selection system for salinity tolerance in sugarcane

Effects of ionizing radiation on the growth and allyl isothiocyanate accumulation of Wasabia japonica in vitro and ex vitro

Red rot resistant gene characterization using RGAP markers among sugarcane cultivars resistant and susceptible to the red rot disease

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