Selection of Specific Peptides for Coccidioides spp. Obtained from Antigenic Fractions through SDS-PAGE and Western Blot Methods by the Recognition of Sera from Patients with Coccidioidomycosis

Escalante, Esperanza Duarte; Frías-De-León, María Guadalupe; García, Luz; Herrera, J.; Altamirano, Gustavo Acosta; Cabello, Carlos; Palma, Gabriel; Montes, María del Rocío Reyes

doi:10.3390/molecules23123145

Cited by 8 publications

(3 citation statements)

References 49 publications

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“…The samples were processed as described previously by Duarte Escalante et al 35 with some modifications. Briefly, the antigenic spots were selected on corresponding 2‐DE gels and cut into very small pieces.…”

Section: Methodsmentioning

confidence: 99%

Identification of differential protein recognition pattern between Naegleria fowleri and Naegleria lovaniensis

Gutiérrez‐Sánchez

Carrasco-Yépez

Herrera‐Díaz

et al. 2020

Parasite Immunology

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Many pathogenicity factors are involved in the development of primary amoebic meningoencephalitis (PAM) caused by N fowleri. However, most of them are not exclusive for N fowleri and they have not even been described in other nonpathogenic Naegleria species. Therefore, the objective of this work was to identify differential proteins and protein pattern recognition between Naegleria fowleri and Naegleria lovaniensis using antibodies anti‐N fowleri as strategy to find vaccine candidates against meningoencephalitis. Electrophoresis and Western blots conventional and 2‐DE were performed for the identification of antigenic proteins, and these were analysed by the mass spectrometry technique. The results obtained in 2‐DE gels and Western blot showed very notable differences in spot intensity between these two species, specifically those with relative molecular weight of 100, 75, 50 and 19 kDa. Some spots corresponding to these molecular weights were identified as actin fragment, myosin II, heat shock protein, membrane protein Mp2CL5 among others, with differences in theoretical post‐translational modifications. In this work, we found differences in antigenic proteins between both species, proteins that could be used for a further development of vaccines against N fowleri infection.

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Section: Methodsmentioning

confidence: 99%

Identification of differential protein recognition pattern between Naegleria fowleri and Naegleria lovaniensis

Gutiérrez‐Sánchez

Carrasco-Yépez

Herrera‐Díaz

et al. 2020

Parasite Immunology

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“…Identification of proteins by mass spectrometry samples was processed as described previously by [ 26 ]. The sample treatment prior to identification was carried out according to the protocol developed in the Research and Industry Support Services Unit (USAII) of the School of Chemistry at the National Autonomous University of Mexico (UNAM).…”

Section: Methodsmentioning

confidence: 99%

Proteomic profile of tepary bean seed storage proteins in germination with low water potential

Padilla-Chacón,

Campos-Patiño,

Peña-Valdivia

et al. 2024

Proteome Sci

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Background Tepary bean (Phaseolus acutifolius A. Gray) is one of the five species domesticated from the genus Phaseolus with genetic resistance to biotic and abiotic stress. To understand the mechanisms underlying drought responses in seed storage proteins germinated on water and polyethylene glycol (PEG-6000) at -0.49 MPa, we used a proteomics approach to identify potential molecular target proteins associated with the low water potential stress response. Methods Storage proteins from cotyledons of Tepary bean seeds germinated at 24, 48 and 72 h on water and PEG-6000 at -0.49 MPa were analyzed by one-dimensional electrophoresis (DE) with 2-DE analysis and shotgun mass spectrometry. Using computational database searching and bioinformatics analyses, we performed Gene Ontology (GO) and protein interactome (functional protein association network) String analyses. Results Comparative analysis showed that the effect of PEG-6000 on root growth was parallel to that on germination. Based on the SDS‒PAGE protein banding patterns and 2-DE analysis, ten differentially abundant seed storage proteins showed changes in storage proteins, principally in the phaseolin and lectin fractions. We found many proteins that are recognized as drought stress-responsive proteins, and several of them are predicted to be intrinsically related to abiotic stress. The shotgun analysis searched against UniProt’s legume database, and Gene Ontology (GO) analysis indicated that most of the seed proteins were cytosolic, with catalytic activity and associated with carbohydrate metabolism. The protein‒protein interaction networks from functional enrichment analysis showed that phytohemagglutinin interacts with proteins associated with the degradation of storage proteins in the cotyledons of common bean during germination. Conclusion These findings suggest that Tepary bean seed proteins provide valuable information with the potential to be used in genetic improvement and are part of the drought stress response, making our approach a potentially useful strategy for discovering novel drought-responsive proteins in other plant models.

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“…A C. immitis specific peptide was selected from the “GPI anchored serine-threonine rich protein OS” that recognized both C. immitis and C. posadasii . These peptides can be employed in diagnostic reagents, immunobiologicals, and antifungal drugs [ 48 ].…”

Section: Coccidioidomycosismentioning

confidence: 99%

Immunologic Diagnosis of Endemic Mycoses

Almeida‐Paes

Bernardes-Engemann

Motta

et al. 2022

JoF

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The endemic mycoses blastomycosis, coccidioidomycosis, histoplasmosis, paracoccidioidomycosis, cryptococcosis, sporotrichosis, talaromycosis, adiaspiromycosis, and emergomycosis are mostly caused by geographically limited thermally dimorphic fungi (except for cryptococcosis), and their diagnoses can be challenging. Usual laboratory methods involved in endemic mycoses diagnosis include microscopic examination and culture of biological samples; however, serologic, histopathologic, and molecular techniques have been implemented in the last few years for the diagnosis of these mycoses since the recovery and identification of their etiologic agents is time-consuming and lacks in sensitivity. In this review, we focus on the immunologic diagnostic methods related to antibody and antigen detection since their evidence is presumptive diagnosis, and in some mycoses, such as cryptococcosis, it is definitive diagnosis.

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Selection of Specific Peptides for Coccidioides spp. Obtained from Antigenic Fractions through SDS-PAGE and Western Blot Methods by the Recognition of Sera from Patients with Coccidioidomycosis

Cited by 8 publications

References 49 publications

Identification of differential protein recognition pattern between Naegleria fowleri and Naegleria lovaniensis

Identification of differential protein recognition pattern between Naegleria fowleri and Naegleria lovaniensis

Proteomic profile of tepary bean seed storage proteins in germination with low water potential

Immunologic Diagnosis of Endemic Mycoses

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