Selection of somatic hybrids between diploid clones of potato (Solanum tuberosum L.) transformed by direct gene transfer

Masson, Jean; Lancelin, Dominique; Bellini, Catherine; Lecerf, Marion; Guerche, Philippe; Pelletier, Georges

doi:10.1007/bf00288791

Cited by 49 publications

(14 citation statements)

References 21 publications

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“…These include selection by micromanipulation (Puite et al, 1986), cell-sorting (Puite et al, 1988), selection based on differences in callus growth of parental clones and their hybrids (Debnath & Wenzel, 1987), and, increasingly, selection using molecular or biochemical markers (de Vries et al, 1987;Masson et al, 1989;Pehu et al, 1989). Each of these techniques has advantages and limitations.…”

Section: Introductionmentioning

confidence: 99%

Identification of somatic hybrids of dihaploid Solanum tuberosum lines and S. brevidens by species specific RAPD patterns and assessment of disease resistance of the hybrids

Rokka¹,

Kankila³

et al. 1994

Euphytica

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Symmetric somatic hybrids were produced by electrofusion of protoplasts of two dihaploid tuber-bearing potato (Solanum tuberosum L.) lines and Solanum brevidens Phil., a diploid non-tuber-bearing wild potato species. A total of 985 plants was obtained. Verification of nuclear hybridity of putative hybrids was based on additive RAPD patterns, general morphological characteristics and chromosome counts. 53 (90%) calli regenerated into plants which were identified as somatic hybrids. Most of the hybrids were aneuploids at the tetraploid (4 x) or hexaploid (6 x ) level. The 20 hybrids tested expressed a high level of resistance to potato virus Y (PVY N) characteristic of the S. brevidens parent. Resistance to late blight (Phytophthora infestans (Mont.) de Bary) varied between hybrids, but was on average better than that of the fusion parents. Resistance of hybrids to bacterial stem rot (Erwinia carotovora subsp, atroseptica (van Hall) Dye) was not superior to that of commercial potato cultivars.

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Section: Introductionmentioning

confidence: 99%

Identification of somatic hybrids of dihaploid Solanum tuberosum lines and S. brevidens by species specific RAPD patterns and assessment of disease resistance of the hybrids

Rokka¹,

Kankila³

et al. 1994

Euphytica

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“…For this purpose, we applied various conditions to poplar protoplasts mixed with 20 ~tg pABD1. The first successful selection for resistance to paromomycin was obtained with protoplasts submitted to three discharges of a 15 IxF capacitor charged at 250 V. It was a surprise since the same procedure was effective for transformation of tobacco (Guerche et al, 1987a), rape (Guerche et al, 1987b), potato (Masson et al, 1989) and lettuce (Chupeau et al, 1989). As poplar protoplasts are smaller (15 ~tm in diameter) than the protoplasts of the other species (about 60 lira), the theory (Neumann et al, 1982) predicted that poplar protoplasts require stronger discharges to be effectively permeabilized.…”

Section: Electroporation Conditionsmentioning

confidence: 99%

Recovery of transgenic trees after electroporation of poplar protoplasts

Chupeau

Pautot

Chupeau

1994

Transgenic Research

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Protoplasts from leaflets of in vitro cuttings were electroporated in osmotically adjusted and buffered solutions containing plasmid DNA: pABD1, carrying the nptlIgene for resistance to neomycin; pGH1, carrying a mutant acetolactate synthase gene, als, for resistance to sulfonylurea; and pGSFR781A, carrying a synthetic phosphinothricin acetyltransferase (pat) for resistance to phosphinothricin (Basta). Gene transfer was repeatedly efficient, without use of carrier DNA, in the range of one transformant for 105 to 104 protoplast-derived cell colonies. This was probably due to the high plating efficiency (30%) of protoplasts in our culture process. Selection for expression of foreign genes was applied in liquid medium and repeatedly achieved with 30 ~M paromomycin for NPTII, 200 nM chlorsulfuron for the mutant ALS of Arabidopsis and 25 ~tM phosphinothricin for PAT expression. Integration of foreign genes into genomic DNA of resistant poplar trees was demonstrated by Southern blot hybridizations, which revealed that for some transformants practically no other part of the vector plasmid than the selected gene was integrated.Effective processes for protoplast culture, efficient selection at the cell colony stage and gene transfer will provide new possibilities in poplar breeding.

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“…Mutants for agronomically-important genotypes are scarce and the production of mutants is both difficult and may have a negative influence on the genetic fidelity of the plant material . The same condition also applies to more recent selection strategies employed where drug resistance genes have been introduced into one or both fusion partners by transformation, although somatic hybrids can be easily identified by selecting for drug resistance (Bates et al ., 1987a ;Masson et al ., 1989 ;Toriyama et al ., 1987a;Wijbrandi et al ., 1990d) .…”

Section: Selection Of Hybridsmentioning

confidence: 99%

The potential of somatic hybridization in crop breeding

Waara

Glimelius

1995

Euphytica

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In recent years, the rapid development of somatic cell genetics has made possible the transfer of alien genes over wide taxonomic distances by somatic hybridization . In this review, the potential of somatic hybridization in the breeding of crops within the Brassicaceae, Fabaceae, Poaceae and Solanaceae is discussed . It is evident from these studies that many hybrids, either symmetric or asymmetric, which are fertile have the potential to be used as a bridge between the alien species and the crop . Progeny analysis of some hybrid combinations also reveals intergenomic translocations which may lead to the introgression of the alien genes . Furthermore, fusion techniques enable the resynthesis of allopolyploid crops to increase their genetic variability and to restore ploidy level and heterozygosity after breeding at reduced ploidy level in polyploid crops.

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Selection of somatic hybrids between diploid clones of potato (Solanum tuberosum L.) transformed by direct gene transfer

Cited by 49 publications

References 21 publications

Identification of somatic hybrids of dihaploid Solanum tuberosum lines and S. brevidens by species specific RAPD patterns and assessment of disease resistance of the hybrids

Identification of somatic hybrids of dihaploid Solanum tuberosum lines and S. brevidens by species specific RAPD patterns and assessment of disease resistance of the hybrids

Recovery of transgenic trees after electroporation of poplar protoplasts

The potential of somatic hybridization in crop breeding

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