Selection of regioselective DNA aptamer for detection of homocysteine in nondeproteinized human plasma

Zhou, Jianshuo; Li, Hui; Li, Jinming; Liu, Xuemei; Zhao, Jiaxing; Wang, N.; Wang, Yuxiao; Yu, Zhuang; Zhang, Xin; Xin, Ying; Li, Xiaoqi; Zheng, Wei; Shao, Ningsheng; Lou, Xinhui

doi:10.1016/j.bios.2023.115528

Cited by 2 publications

(3 citation statements)

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“…Optical fibers were treated and functionalized according to a reported method [32] with minor modifications. Briefly, approximately 3.5 cm of the resin layer of a fiber was removed.…”

Section: Fabrication Of the Foew Aptasensormentioning

confidence: 99%

“…MCP-SELEX was initially used for the isolation of protein-binding aptamers. Recently, it has been used for the efficient isolation of small molecule-binding aptamers with high specificity and affinity [32]. There have hitherto been no reports on the application of MCP-SELEX to the synthesis of inactivated virus-binding aptamers.…”

Section: Introductionmentioning

confidence: 99%

“…A fluorophore, which is typically labeled on the recognition ligand or target, is attached to the surface of optical fibers. FOEW sensors offer advantages such as low cost, ease of operation, automated data collection, a small size, and easy maintenance [32,33]. FOEW aptasensors have been analyzed for the sensitive detection of various types of targets, including heavy metal ions [34], proteins [33], and exosomes [35], but not whole inactivated viruses.…”

Section: Introductionmentioning

confidence: 99%

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Rapid and Sensitive Detection of Inactivated SARS-CoV-2 Virus via Fiber-Optic and Electrochemical Impedance Spectroscopy Based Aptasensors

Xiao,

Wang,

Zhao

et al. 2024

Biosensors

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The development of rapid detection tools for viruses is vital for the prevention of pandemics and biothreats. Aptamers that target inactivated viruses are attractive for sensors due to their improved biosafety. Here, we evaluated a DNA aptamer (named as 6.9) that specifically binds to the inactivated SARS-CoV-2 virus with a low dissociation constant (KD = 9.6 nM) for the first time. Based on aptamer 6.9, we developed a fiber-optic evanescent wave (FOEW) biosensor. Inactivated SARS-CoV-2 and the Cy5.5-tagged short complementary strand competitively bound with the aptamer immobilized on the surface of the sensor. The detection of the inactivated SARS-CoV-2 virus was realized within six minutes with a limit of detection (LOD, S/N = 3) of 740 fg/mL. We also developed an electrochemical impedance aptasensor which exhibited an LOD of 5.1 fg/mL and high specificity. We further demonstrated that the LODs of the FOEW and electrochemical impedance aptasensors were, respectively, more than 1000 and 100,000 times lower than those of commercial colloidal gold test strips. We foresee that the facile aptamer isolation process and sensor design can be easily extended for the detection of other inactivated viruses.

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“…Optical fibers were treated and functionalized according to a reported method [32] with minor modifications. Briefly, approximately 3.5 cm of the resin layer of a fiber was removed.…”

Section: Fabrication Of the Foew Aptasensormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%