Selection of reference genes and expression of key genes involved in response to short-term soil flooding stress in Prunus persica rootstock ‘Tsukuba 1’

da Silva, Flávia Lourenço; Lucho, Simone Ribeiro; Klumb, Elsa Kuhn; Bianchi, Valmor João

doi:10.1016/j.genrep.2023.101843

Gene Reports

2023

DOI: 10.1016/j.genrep.2023.101843

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Selection of reference genes and expression of key genes involved in response to short-term soil flooding stress in Prunus persica rootstock ‘Tsukuba 1’

Flávia Lourenço da Silva,

Simone Ribeiro Lucho,

Elsa Kuhn Klumb

et al.

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2024

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Cited by 1 publication

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Evaluation of Suitable Reference Genes for Quantitative Real-Time PCR in Various Tissues of Apocynum venetum

Li,

Zhang,

Jiang

et al. 2024

Genes

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Apocynum venetum L. is an economically valuable plant with tolerance to drought and salinity. Its leaves are utilized in tea production and pharmaceuticals, while the stem bark serves as a high-quality fiber material. To gain insights into the gene expression patterns of A. venetum using quantitative real-time PCR (qRT-PCR), it is crucial to identify appropriate reference genes. This study selected nine candidate genes, including α-tubulin (TUA), β-tubulin (TUB), actin (ACT), cyclophilin (CYP), elongation factor-1α (EF-1α), the B family of regulatory subunits of protein phosphatase (PPP2R2, PPP2R3, and PPP2R5), and phosphoglycerate kinase (PGK), to determine the most appropriate reference genes in the leaf, stem, and root tissues of A. venetum. A comprehensive ranking by geNorm, NormFinder, BestKeeper, and RefFinder software and Venn diagrams was used to screen more stable reference genes in different tissues. The two most stable reference genes were CYP and TUA in leaves, PGK and PPP2R3 in stems, and TUA and EF-1α in roots, respectively. The relative expression values of the four genes involved in proline metabolism under polyethylene glycol treatment were used to validate the screened reference genes, and they exhibited highly stable expression levels. These findings represent the first set of stable reference genes for future gene expression studies in A. venetum. They significantly contribute to enhancing the accuracy and reliability of gene expression analyses in this economically important plant species.

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Evaluation of Suitable Reference Genes for Quantitative Real-Time PCR in Various Tissues of Apocynum venetum

Li,

Zhang,

Jiang

et al. 2024

Genes

View full text Add to dashboard Cite

show abstract

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Selection of reference genes and expression of key genes involved in response to short-term soil flooding stress in Prunus persica rootstock ‘Tsukuba 1’

Cited by 1 publication

References 53 publications

Evaluation of Suitable Reference Genes for Quantitative Real-Time PCR in Various Tissues of Apocynum venetum

Evaluation of Suitable Reference Genes for Quantitative Real-Time PCR in Various Tissues of Apocynum venetum

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