Selection of Pseudomonas aeruginosa for biosurfactant production and studies of its antimicrobial activity

El-Sheshtawy, H.S.; Doheim, M. M.

doi:10.1016/j.ejpe.2014.02.001

Cited by 87 publications

(49 citation statements)

References 40 publications

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“…The carbon source for maximum ST reduction, biosurfactant production, along with highest antifungal activity of the biosurfactant was observed to be the medium containing glucose which is in agreement with earlier reports . Bacterial supernatant was used to test the antifungal activity of biosurfactant against C. falcatum as it contains crude biosurfactant product .…”

Section: Discussionsupporting

confidence: 86%

Antifungal properties of rhamnolipid produced by Pseudomonas aeruginosa DS9 against Colletotrichum falcatum

Goswami

Borah

Lahkar

et al. 2015

J. Basic Microbiol.

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The rhamnolipid biosurfactant (RL-DS9) extracted from the bacterial strain Pseudomonas aeruginosa DS9 was evaluated for its antifungal activity against Colletotrichum falcatum that causes red rot in sugarcane. The surface tension (ST) reduction, biosurfactant production, and antifungal activity of biosurfactant against C. falcatum were investigated by using the medium with different carbon sources and it was found to be maximum in glucose. Moreover, highest reduction of ST and production of biosurfactant was achieved at 4.5% (w/v) concentration of glucose. The efficacy of RL-DS9 was compared with a commercially available rhamnolipid (RL-R95) using microtitre plate assay. Results showed that at 100 μg ml(-1) concentration RL-DS9 exhibited 86.6% inhibition against C. falcatum spore germination, and in the same concentration RL-R95 showed 83.3% inhibition. From liquid chromatography-mass spectrometry (LC-MS) analysis, it was revealed that only two similar congeners Rha-(C10 ) and Rha-Rha-(C10:1 ) were found to be in common among both the rhamnolipids. In the plant bioassay test, it was noted that red rot incidence was reduced on the sugarcane plants treated with RL-DS9. This is the first report that rhamnolipid biosurfactant produced by Pseudomonas aeruginosa DS9 could be able to control red rot disease of sugarcane caused due to the infection with the fungus Colletotrichum falcatum.

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Section: Discussionsupporting

confidence: 86%

Antifungal properties of rhamnolipid produced by Pseudomonas aeruginosa DS9 against Colletotrichum falcatum

Goswami

Borah

Lahkar

et al. 2015

J. Basic Microbiol.

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“…Production of biosurfactant is typically affected by carbon and nitrogen sources, pH and the salinity of the medium [6]. In this study, palm oil was used as the main carbon source, while for the nitrogen source, it was varied to obtain the optimum production of biosurfactant by H. meridiana BK-AB4.…”

Section: Optimization Of Biosurfactant Productionmentioning

confidence: 99%

“…The other alternative is the used of biosurfactant, which is surfactant produced by certain microorganisms that are cultivated in the lipid-rich medium. Biosurfactant has some advantages than the synthetic one, such as low toxicity, easier to decompose and reproducible [5][6][7]. In addition, biosurfactants have similar functions to surfactant synthetic.…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of Palm Oil into Biosurfactant by <i>Halomonas meridiana</i> BK-AB4 for the Application of Corrosion Inhibitor

2018

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Biosurfactant is environmentally friendly surfactant produced by a certain microorganism in a lipid-rich medium. A previous study has shown that Halomonas meridiana BK-AB4 had the potential of a moderately halophilic bacterium in converting olive oil into biosurfactant. In the present study, the effect of changing the carbon source from olive oil into cheaper and more abundant vegetable oil, which is palm oil, for the production of the biosurfactant was evaluated. The study began by optimizing the production medium with varying the nitrogen source, the concentration of palm oil and pH. The optimum condition of biosurfactant production was observed in the medium consisted of 0.6% (w/v) of urea, 2% (v/v) of palm oil and pH 9. The resulted biosurfactant was stable at pH 7-10 and in the salt concentration of 6-15%. Biosurfactant activity in lowering air-water surface tension was measured using the Du Noüy ring method, and the value of critical micelle concentration (CMC) was about 233 ppm. At this point, the surface tension of water dropped from 68.3 to 49.8 dyne/cm. Preliminary structural analysis by using FTIR technique suggested that the resulted biosurfactant has -OH, -C-H aliphatic C=C, H-C-C and C=O groups in its structure, which is similar to that of the fatty-acid type of biosurfactant. The potential of biosurfactant as a metal corrosion inhibitor was evaluated by using electrochemical impedance spectroscopy (EIS) that measured at 30 °C. The measurement revealed that the highest inhibition level was observed at the biosurfactant concentration about 200 ppm that corresponds to the inhibition level about 53.23%. ABSTRAKBiosurfaktan adalah surfaktan ramah lingkungan yang diproduksi oleh mikroorganisme tertentu dalam media kaya lemak. Dalam studi sebelumnya, telah ditemukan potensi bakteri halofilik, Halomonas meridiana BK-AB4, dalam mengkonversi minyak zaitun ke biosurfaktan. Dalam penelitian ini dipelajari efek variasi sumber karbon dari minyak zaitun ke minyak yang lebih murah dan berlimpah, yaitu minyak sawit, untuk produksi biosurfaktan. Studi ini dimulai dengan mengoptimalkan produksi media pada berbagai sumber nitrogen, konsentrasi minyak sawit dan pH. Kondisi optimum produksi biosurfaktan diamati dalam medium yang terdiri dari 0,6% (b/v) urea, 2% (v/v) minyak sawit dan pH 9. Produksi biosurfaktan stabil pada pH 7-10 dan konsentrasi garam 6-15%. Aktifitas biosurfaktan dalam menurunkan tegangan permukaan air-air diukur menggunakan metode cincin Du Noüy, nilai konsentrasi Misel kritis (CMC) adalah sekitar 233 ppm. Pada titik ini, tegangan permukaan air menurun dari 68,3 ke 49.8 dyne/cm. Analisis awal struktur menggunakan teknik FTIR dihasilkan bahwa biosurfaktan memiliki gugus -OH, -C-H alifatik C=C, H-C-C dan C=O dalam strukturnya, yang mirip dengan jenis asam lemak biosurfaktan. Potensi biosurfaktan sebagai inhibitor korosi logam dievaluasi menggunakan spektroskopi impedansi elektrokimia (EIS) yang diukur di 30 °C. Pengukuran menunjukkan bahwa tingkat inhibisi diamati pada konsentrasi biosurfaktan sekitar ...

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“…As a comparison, biosurfactants have demonstrated significant structural diversity, lower toxicity and production conditions and higher biodegradability, foaming ability, selectivity and specific activity at extreme conditions [11,12]. Pharmaceutical applications include antimicrobial, anti-cancer and anti-biofilm properties, the former and latter promoting an alternative approach to synthetic antibiotics [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Production of Mannosylerythritol Lipids (MELs) to be Used as Antimicrobial Agents Against S. aureus ATCC 6538

et al. 2020

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Antimicrobial resistance (AMR) is a current major health issue, both for the high rates of resistance observed in bacteria that cause common infections and for the complexity of the consequences of AMR. Pathogens like Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Mycobacterium tuberculosis among others are clear examples of antibiotic-resistant threats. Biosurfactants have recently emerged as a potential new generation of anti-adhesive and anti-biofilm agents; mannosylerythritol lipids (MELs) are biosurfactants produced by a range of fungi. A range of structural variants of MELs can be formed and the proportion of each isomer in the fermentation depends on the yeast used, the carbon substrate used for growth and the duration of the fermentation. In order to allow assessment of the possible functions of MELs as antimicrobial molecules, small quantities of MEL were produced by controlled fermentation. Fermentations of the yeast Pseudozyma aphidis using rapeseed oil as a carbon source yielded up to 165 g MELs /kg Substrate. The MELs formed by this strain was a mixture of MEL-A, MEL-B, MEL-C and MEL-D. The MELs produced were tested against S. aureus ATCC 6538 on pre-formed biofilm and on co-incubation biofilm experiments on silicone discs; showing a disruption of biomass, reduction of the biofilm metabolic activity and a bacteriostatic/bactericidal effect confirmed by a release of oxygen uptake p O 2 , the reduction of citrate synthase activity and scanning electron microscopy. The results show that MELs are promising antimicrobial molecules for biomedical technological applications that could be studied in detail in large-scale systems and in conjunction with animal tissue models.

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Selection of Pseudomonas aeruginosa for biosurfactant production and studies of its antimicrobial activity

Cited by 87 publications

References 40 publications

Antifungal properties of rhamnolipid produced by Pseudomonas aeruginosa DS9 against Colletotrichum falcatum

Antifungal properties of rhamnolipid produced by Pseudomonas aeruginosa DS9 against Colletotrichum falcatum

Bioconversion of Palm Oil into Biosurfactant by <i>Halomonas meridiana</i> BK-AB4 for the Application of Corrosion Inhibitor

Production of Mannosylerythritol Lipids (MELs) to be Used as Antimicrobial Agents Against S. aureus ATCC 6538

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