Selection of probiotic bifidobacteria for lambs

Vlková, Eva; Grmanová, M.; Rada, V.; Homutová, I.; Dubná, Soňa

doi:10.17221/151/2009-cjas

Cited by 14 publications

(9 citation statements)

References 41 publications

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“…Three days after the application of probiotics, the number of lactobacilli was insignificantly higher in both experimental groups compared to the control, but the differences were not significant (Table 1). This finding is consistent with the results obtained by Vlkova et al (2009), who observed a slight increase in lactobacilli numbers after feeding bifidobacteria to lambs.…”

Section: Discussionsupporting

confidence: 93%

Persistence of bifidobacteria in the intestines of calves after administration in freeze-dried form or in fermented milk

Geigerová¹,

Vlková²,

Bunešová³

et al. 2016

CZECH J ANIM SCI

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ABSTRACT:In order to improve the gut microbiome of calves, probiotic bacteria can be fed as active livingcells (fermented milk), or as live but inactive (freeze-dried) cultures. Ten bifidobacterial strains with suitable probiotic properties (as determined in our previous study) were tested for survival during the freeze-drying process, and screened for their ability to ferment cow's milk. The viability of both freeze-dried and live-cell cultures during storage was also tested. All of the strains tested were able to ferment cow's milk, with average counts of 8.26 ± 0.62 log CFU/ml. Eight out of the ten strains were able to survive in milk for 2 months in counts higher than 10 6 CFU/ml. Bifidobacteria showed high viability following the freeze-drying process, with average numbers of 9.03 ± 0.22 log CFU/vial and did not decrease after 12 months of storage. The mixture of rifampicin-resistant variants of bifidobacteria (RRBs) was fed to 2-day-old dairy Charolais calves in the form of living-cells, or as freeze-dried bacteria. The control group was given no probiotics. Survival of the RRBs administered and the numbers of other bacterial groups in faecal samples was monitored by culturing. Bifidobacteria that were administered passed successfully through the upper parts of the gastrointestinal tract, and were found in numbers higher than 10 9 CFU/g for two weeks. RRBs colonized the intestines of calves for at least 63 days in both treatment groups. Significantly higher total counts of bifidobacteria were found in the treated groups, compared to the control group. Reduction in Escherichia coli and total coliforms numbers, and an increase in lactobacilli counts were observed in both experimental groups following the application of the probiotic mixtures. Our results show that both forms of administering probiotic bifidobacteria to calves are effective, but that the freeze-dried form is more suitable from a practical viewpoint.

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Section: Discussionsupporting

confidence: 93%

Persistence of bifidobacteria in the intestines of calves after administration in freeze-dried form or in fermented milk

Geigerová¹,

Vlková²,

Bunešová³

et al. 2016

CZECH J ANIM SCI

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“…Properties predicting survival of lactobacilli during passage through the gastrointestinal tract (acid and bile tolerance) were tested as described elsewhere [62]. Briefly, overnight bacterial suspension free of cultivation media was mixed with oxygen-free phosphate buffered saline (OF-PBS) of pH 2 or 3 (pH adjusted with HCl), with OF-PBS buffer (pH 7.2) containing 1%, 2%, or 3% bile extract, or with OF-PBS buffer (pH 7.2) for control.…”

Section: Methods Of Isolation Characterization and Identification Omentioning

confidence: 99%

Colonization of Germ-Free Piglets with Commensal Lactobacillus amylovorus, Lactobacillus mucosae, and Probiotic E. coli Nissle 1917 and Their Interference with Salmonella Typhimurium

et al. 2019

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Non-typhoid Salmonellae are worldwide spread food-borne pathogens that cause diarrhea in humans and animals. Their multi-drug resistances require alternative ways to combat this enteric pathogen. Mono-colonization of a gnotobiotic piglet gastrointestinal tract with commensal lactobacilli Lactobacillus amylovorus and Lactobacillus mucosae and with probiotic E. coli Nissle 1917 and their interference with S. Typhimurium infection was compared. The impact of bacteria and possible protection against infection with Salmonella were evaluated by clinical signs, bacterial translocation, intestinal histology, mRNA expression of villin, claudin-1, claudin-2, and occludin in the ileum and colon, and local intestinal and systemic levels of inflammatory cytokines IL-8, TNF-α, and IL-10. Both lactobacilli colonized the gastrointestinal tract in approximately 100× lower density compare to E. coli Nissle and S. Typhimurium. Neither L. amylovorus nor L. mucosae suppressed the inflammatory reaction caused by the 24 h infection with S. Typhimurium. In contrast, probiotic E. coli Nissle 1917 was able to suppress clinical signs, histopathological changes, the transcriptions of the proteins, and the inductions of the inflammatory cytokines. Future studies are needed to determine whether prebiotic support of the growth of lactobacilli and multistrain lactobacilli inoculum could show higher protective effects.

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“…Three published studies support our finding, describing the identification of Bifidobacteria in sheep and the use of this microorganism as a potential probiotic. [42][43][44] In humans, they are generally thought to be a marker of good gut health [41].…”

Section: Discussionmentioning

confidence: 99%

Interactions between Teladorsagia circumcincta Infections and Microbial Composition of Sheep with or without Successful Monepantel Treatment—A Preliminary Study

et al. 2021

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Nematodes are one of the main impactors on the health, welfare and productivity of farmed animals. Teladorsagia circumcincta are endemic throughout many sheep-producing countries, particularly in the northern hemisphere, and contribute to the pathology and economic losses seen on many farms. Control of these nematode infections is essential and heavily reliant on chemotherapy (anthelmintics), but this has been compromised by the development of anthelmintic resistance. In mammals, the composition of the intestinal microbiota has been shown to have a significant effect on overall health. The interactions between host, microbiota and pathogens are complex and influenced by numerous factors. In this study, comparisons between intestinal and faecal microbiota of sheep infected with sensitive or resistant strains of T. circumcincta, with or without monepantel administration were assessed. The findings from both faecal samples and terminal ileum mucosal scrapings showed clear differences between successfully treated animals and those sheep that were left untreated and/or those carrying resistant nematodes. Specifically, the potentially beneficial genus Bifidobacterium was identified as elevated in successfully treated animals. The detection of these and other biomarkers will provide the basis for new therapeutic reagents particularly relevant to the problems of emerging multidrug anthelmintic resistance.

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Selection of probiotic bifidobacteria for lambs

Cited by 14 publications

References 41 publications

Persistence of bifidobacteria in the intestines of calves after administration in freeze-dried form or in fermented milk

Persistence of bifidobacteria in the intestines of calves after administration in freeze-dried form or in fermented milk

Colonization of Germ-Free Piglets with Commensal Lactobacillus amylovorus, Lactobacillus mucosae, and Probiotic E. coli Nissle 1917 and Their Interference with Salmonella Typhimurium

Interactions between Teladorsagia circumcincta Infections and Microbial Composition of Sheep with or without Successful Monepantel Treatment—A Preliminary Study

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