“…In soil microbial sequencing, a bacterial genome’s 16S rRNA gene fragment is first amplified and sequenced, followed by studying the colony type and microorganism structure through bioinformatic analyses ( 3 , 5 , 7 , 8 ). Recently, with the development of research and technology, second-generation high-throughput sequencing (NGS) technology has emerged, which is a modification of the traditional Sanger sequencing ( 9 – 11 ). This technology can sequence many microbial DNA molecules in the V4 region of a soil sample in parallel in a single run and has a substantial quantitative capability, where the number of times a DNA sample is sequenced directly reflects the DNA abundance of that sample.…”