Selection of peptides that bind to the core oligosaccharide of R-form LPS from a phage-displayed heptapeptide library

Abstract

To characterize common sites within the core oligosaccharide of the R-form lipopolysaccharide (LPS), we screened peptides from a phage-displayed heptapeptide library by using the most truncated form of R-LPS, Re-LPS (S. Typhimurium SL1165) as a ligand. After three rounds of biopanning/amplification and subsequent screening by phagemid enzyme-linked immunosorbent assay (ELISA), we selected three distinct clones that bind to the ligand LPS. We characterized the binding sites of the three clones by ELISA and thin-layer chromatography immunostaining and found that the three clones bind the two Re-LPSs (SL1165 and S. Minnesota Re595) and Rb2-LPS. In addition, one of the clones also bound to S-form LPS (S. Enteritidis). Current data show that those clones bind to common carbohydrate structure(s) expressed in the core oligosaccharides of those LPS samples.

The Oligosaccharide of Gonococcal Lipooligosaccharide Contains Several Epitopes That Are Recognized by Human Antibodies

“…We have observed similar binding patterns with peptides screened from a phage-displayed library using the same Re LPS as a ligand; the peptides selected from the LPS bound to Rb but not to Ra, Rc, or Rd (29). It has also been reported by other investigators that one of the murine MAbs raised against Re mutant LPS binds to Rb2 LPS (26).…”

Development of Anti-Infectives Using Phage Display: Biological Agents against Bacteria, Viruses, and Parasites

“…Neutralization of LPS is one of the most promising targets for the development of novel antimicrobial agents where phage display could provide an approach for selection of antibodies and peptides specific for LPS. Several antibodies (43,48,85) and peptides (44,59,60,78,92,169) against LPS from different bacteria strains have been identified. Furthermore, purified lipid A can also be used as a target for phage display screening (137).…”

Combinatorial discovery of tumor targeting peptides using phage display

“…Noda et al [2001] used phage display to identify peptides specific for carbohydrates, including lipopolysaccharide. Competition experiments demonstrated that phage displaying three peptides (RVVKESR, YSALEEG, MMGVGTS) bound to different sites on the core oligosaccharide structure of the Salmonella lipopolysaccharide molecule [Noda et al, 2001].…”