To overcome the side effects of conventional chemotherapy, the study of effective anticancer application of magnetic iron oxide nanoparticles is now-a-days a great area of research which is increasing day by day. In our present study, Iron oxide nanoparticles (IONPs) have been synthesised by reducing ferrous sulphate using Punica granatum peel extracts. Punica granatum peel extract has a rich source of flavonoids and phenolic compounds with high antioxidant and these molecules can be used as excellent reducing agents. The nanopowders have been calcined at 700 0 C for 4 hours. Physicochemical, optical, photocatalytic, cytotoxicity activities (both in normal and carcinoma cell) and morphological assessment of carcinoma cells are characterised using analytical techniques. XRD data, DLS, FTIR spectra and Scanning electron microscope analysis have confirmed the formation of IONPs having spherical shape with trigonal (rhombohedral axes) phase structure, smaller size (average size from XRD 43-47 nm) and large surface area. From UV-Vis absorption spectra and Tauc plot the optical band gap has been obtained 3.5 e.V. IONPs show good photo-Fenton in Methylene blue (MB) dye degradation within 15 minutes with ~91% and 40 minutes with 99% for under optimum condition. The cytotoxicity in Human hepatic cell line (WRL-68) and human hepatocellular carcinoma (HUH-7) cell lines have also been observed using MTT assay and verified Green Synthesis … Shatarupa Basak et al.