Selection of optimal conditions for separating lignan-containing extract from oil flax seed by thin-layer chromatography

Abstract

The optimum conditions for separating a lignan-containing extract from seeds of the oil flax (Linum usitatissimum) by thin-layer chromatography were identified. Elution using the method proposed here allowed identification of a brilliant blue fluorescent spot associated with the main lignan, which was secoisolariciresinol diglucoside (SDG), giving clear identification of this component in mixtures even without a standard. This method can be used for qualitative express analysis of phytopreparations based on lignans, with the aim of detecting adulterated phytopreparations and biologically active additives.