Selection of monoclonal antibodies for the identification of lymphocyte surface antigens in the New World primate Saguinus oedipus oedipus (cotton top tamarin)

An extensive monoclonal antibody panel for the phenotyping of leukocyte subsets in the common marmoset and the cotton-top tamarin

“…Although several reports have been published for the common marmoset (26,27), data are very limited in the cotton-top tamarin (23)(24)(25). The results of this study are summarized in Table 1.…”

“…In contrast, CD19 (4G7; Becton Dickinson) showed positive cross-reactivity when conjugated with PE or APC, but not with FITC. Although different labeling methods are available for coupling the fluorescein to the mAb, to our knowledge, no satisfactory explanation has been published despite the fact that this phenomenon has been reported frequently (23,26,27). One could envisage that under certain experimental conditions, quenching or oxidation/reduction of the fluorochrome can take place.…”

“…One way to characterize cells is to use flow cytometric analysis using monoclonal antibodies (mAb) directed to cell surface antigens. Few studies have reported on the cross-reactivity of mAbs, raised originally against human CD epitopes, in the cotton-top tamarin (23)(24)(25) and common marmoset (26,27). The most extensive study to date (24) reported 62% cross-reactivity of 121 mAbs tested.…”

Virus-specific cytotoxic T cell responses are associated with immunity of the cottontop tamarin to Epstein–Barr virus (EBV)

“…Fortuitously, B152 and B158 were two individuals who showed little cross-reactivity, either directly or in the cold target inhibition assay, and this is consistent with the view that some tamarins will not share MHC alleles and demonstrate genetic restriction. We further examined the MHC restriction of the cytotoxic response using MoAbs to block killing by CD4 dual-positive phenotype remains unclear, as this cell phenotype is rare in normal tamarin blood and does not appear on tamarin Con A blasts [9]. Dual-positive cells were detected at lower frequency in human T cell cultures stimulated with LCL (data not shown).…”

“…The phenotype of tamarin cell populations was determined by flow cytometry using cross-reacting mouse anti-human MoAbs as previously described [9]. Anti-CD2 (MT910), CD4 (MT301), CD14 (TUK4), CD20 (B-Lyl), CD23 (MHM6), and CD25 (ACT-1) were purchased from Dako (High Wycombe, UK).…”