Selection of Mercury-Resistant PGPR Strains Using the BMRSI for Bioremediation Purposes

González, Daniel; Mora, Marina Robas; Probanza, A.; Jiménez, Pedro A.

doi:10.3390/ijerph18189867

Cited by 14 publications

(11 citation statements)

References 48 publications

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“…In the present study, four strains have been used whose PGP activities were tested in media with the presence of Hg vs. the absence of Hg. In the same way, their respective consortia were tested in pairs ( González et al, 2021b ).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the oxidative stress alleviation in Lupinus albus var. orden Dorado by the inoculation of four plant growth-promoting bacteria and their mixtures in mercury-polluted soils

et al. 2022

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Mercury (Hg) pollution is a serious environmental and public health problem. Hg has the ability to biomagnify through the trophic chain and generate various pathologies in humans. The exposure of plants to Hg affects normal plant growth and its stress levels, producing oxidative cell damage. Root inoculation with plant growth-promoting bacteria (PGPB) can help reduce the absorption of Hg, minimizing the harmful effects of this metal in the plant. This study evaluates the phytoprotective capacity of four bacterial strains selected for their PGPB capabilities, quantified by the calculation of the biomercuroremediator suitability index (IIBMR), and their consortia, in the Lupinus albus var. orden Dorado. The oxidative stress modulating capacity in the inoculated plant was analyzed by measuring the activity of the enzymes catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR). In turn, the phytoprotective capacity of these PGPBs against the bioaccumulation of Hg was studied in plants grown in soils highly contaminated by Hg vs. soils in the absence of Hg contamination. The results of the oxidative stress alleviation and Hg bioaccumulation were compared with the biometric data of Lupinus albus var. orden Dorado previously obtained under the same soil conditions of Hg concentration. The results show that the biological behavior of plants (biometrics, bioaccumulation of Hg, and activity of regulatory enzymes of reactive oxygen species [ROS]) is significantly improved by the inoculation of strains B1 (Pseudomonas moraviensis) and B2 (Pseudomonas baetica), as well as their corresponding consortium (CS5). In light of the conclusions of this work, the use of these strains, as well as their consortium, is postulated as good candidates for their subsequent use in phytostimulation and phytoprotection processes in areas contaminated with Hg.

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Section: Discussionmentioning

confidence: 99%

“…The PGPB capacity in the presence of Hg of the four bacterial isolates ( Table 1 ) was analyzed by González et al (2021b) (BRMSI Supplementary Table 1 ). The activity of the four strains was tested, as well as the combination consortium in pairs ( Table 2 ).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the oxidative stress alleviation in Lupinus albus var. orden Dorado by the inoculation of four plant growth-promoting bacteria and their mixtures in mercury-polluted soils

et al. 2022

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“…PGPR assist the detoxi cation of mercury in soil by promoting plant growth and increasing the amount of metal reduced. The mercury-resistance and plant growth-promoting activity are crucial characteristics of bacteria that are useful in metal biodetoxi cation [33].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Mercury Ameliorating Rhizobacteria for Enhancing Growth and Yield of Triticum Aestivum L. in Field. An In Vitro and In Silico Study

Amin

Naveed

Rasheed

et al. 2022

Preprint

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Mercury resistant (HgR) and indole-3-acetic acid (IAA) producing rhizobacteria were isolated from mercury-contaminated areas near Itehad Chemicals (PVT) Limited, Kala Shah Kaku, District Sheikhupura and tanneries of district Kasur, Punjab, Pakistan. Out of 60 mercury-resistant bacterial isolates, three were selected based on high mercury resistance (20–40 µg/ml) and IAA production (15–40 µg/ml). Selected isolates were subjected to biochemical and molecular characterization. HPLC and GC-MS analyses were performed for the confirmation of IAA production by selected rhizobacterial in methanol extract. Pot and field experiments were conducted under controlled conditions on Triticum aestivum L. with a bacterial consortium consisting of AZ-3, Z-A15, and Z-A22. Selected bacterial isolates were identified as Bacillus cereus AZ-3, Enterobacter cloacae Z-A15, and Pseudomonas putida Z-A22. B. cereus AZ-3 showed 90% resistance against HgCl2 at 40 µg/ml due to the presence merT gene. E. cloacae Z-A15 and P. putida Z-A22 showed high production of IAA at 20 and 36 µg/ml respectively. High performance liquid chromatography (HPLC), and gas chromatography-mass spectrometry (GC-MS) confirmed the production of IAA by selected bacteria. Greenhouse experiment showed plant growth promoting ability of mercury resistant bacterial isolates with T. aestivum in both HgCl2 amended as well as in HgCl2 non-amended soils. Inoculation of bacterial consortium A7 (AZ-3, Z-A15, and Z-A22-Hg) posed a substantial increase such as 90, 3.2, 19.2, 70.4, and 13.2 in shoot length, tillers, spike length, number of spikelets/spike and seed weight/200 g respectively, as compared to (AZ-3, Z-A22, and Z-A15 + Hg). Field experiments showed 17, 40, 67, 13, 27, and 70% increases in shoot length, dry weight, number of tillers, spike length, number of spikelets, and yield per acre respectively in T. aestivum. In silico analysis showed the structural determination of MerT protein encoded by the merT gene of B. cereus AZ-3 (OM039465) using Domain and Motif analysis, physiochemical features, secondary and tertiary structure prediction, and structure validation by Ramachandran plot. These bioinformatics tools predicted the structural-based functional homology of MerT transmembrane protein associated with mer operon harboring bacteria involved in the Hg-detoxification system. It is concluded that the selected bacterial consortium A7 of the present study can be used as an excellent biofertilizer for lessening Hg pollution and promoting plant growth in Hg-contaminated soil to maintain sustainable agricultural land.

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“…Likewise, it is necessary to know the Hg minimal bactericidal concentration (MBC) as a measure of its mercurotolerant capacity (Mathema et al 2011 ). To assess these factors in an integrated wayRobas et al ( 2021a ); González et al ( 2021 ) propose the use of the BMRSI (Biomercuroremedial Suitability Index):…”

Section: Microorganisms and New Tools For Bioremediationmentioning

confidence: 99%

Bioremediation of environments contaminated with mercury. Present and perspectives

González-Reguero

Mora

Lobo

et al. 2023

World J Microbiol Biotechnol

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Mercury is a highly toxic heavy metal whose emission sources can be both natural and the result of anthropic activity. Its polluting action on soils, and its ability to spread through the atmosphere and aquatic environments, constitutes a threat to human and environmental health; both for its bioaccumulation capacity and for biomagnification through the trophic chain. For this reason, there is a growing scientific and social interest in the reduction of this heavy metal in ecosystems. Bioremediation based on the use of microorganisms and/or plants is postulated as a sustainable alternative to traditional physicochemical methods. The main strategies used for this purpose (individually or in combination) are the volatilization of the contaminant, biosorption, phytoextraction and phytoremediation. All these tools are based on taking advantage of the natural and evolutionary capacity that different organisms have developed to adapt to the presence of various pollutants in the environment. Based on the consulted bibliography, these bioremediation methodologies focus on the use of microorganisms (freely or associated with plants) have been successfully applied in different ecosystems, postulating themselves as a respectful alternative for the future for the recovery of degraded environments. For these reasons there is a growing interest in the scientific community to design and use new techniques in a “One Health” context, which allow interpreting the positive impact of bioremediation. In this sense, the universalization of Omics techniques has allowed to abound in the knowledge of new bacterial taxa, and their biotechnological application. This study pretends to cover the present knowledge about mercury bioremediation techniques. In the same way, some new techniques and perspectives are presented in order to expand the frontiers of future research.

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Selection of Mercury-Resistant PGPR Strains Using the BMRSI for Bioremediation Purposes

Cited by 14 publications

References 48 publications

Evaluation of the oxidative stress alleviation in Lupinus albus var. orden Dorado by the inoculation of four plant growth-promoting bacteria and their mixtures in mercury-polluted soils

Evaluation of the oxidative stress alleviation in Lupinus albus var. orden Dorado by the inoculation of four plant growth-promoting bacteria and their mixtures in mercury-polluted soils

Characterization of Mercury Ameliorating Rhizobacteria for Enhancing Growth and Yield of Triticum Aestivum L. in Field. An In Vitro and In Silico Study

Bioremediation of environments contaminated with mercury. Present and perspectives

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