Selection of Media for Isolation of Streptomycetes

Küster, Eberhard; Williams, Stanley T.

doi:10.1038/202928a0

Cited by 776 publications

(338 citation statements)

References 6 publications

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“…The suspension was diluted with 45 ml saline containing 1.5% phenol (w/v) and shaken with glass beads for 30 minutes at 30°C. After filtration through sterile glass wool and dilution with saline (10 Ϫ1 , 10 Ϫ2 and 10 Ϫ3 , respectively), 0.1 ml aliquots were spread onto starch -casein agar [9], containing cycloheximide (50 m g/ml), nystatin (50 m g/ml), nalidixic acid (20 mg/ml) and novobiocin (20 mg/ml). The plates were incubated 14 days at 27°C, and the resulting colonies were transferred to starch -casein agar and ISP-2 agar [10].…”

Section: Microorganismmentioning

confidence: 99%

Retymicin, Galtamycin B, Saquayamycin Z and Ribofuranosyllumichrome, Novel Secondary Metabolites from Micromonospora sp. Tü 6368

et al. 2005

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A new xanthone compound named retymicin (1) was isolated together with galtamycin B (2) and saquayamycin Z (3), new members of the galtamycin and saquayamycin families, respectively, and the new lumichrome derivative 1-(a-ribofuranosyl)-lumichrome (4) from Micromonospora strain Tü 6368, isolated from a soil sample collected in Romania. Retymicin, galtamycin B and saquayamycin Z show cytostatic effects to various human tumor cell lines whereas saquayamycin Z is also active against Gram-positive bacteria.

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Section: Microorganismmentioning

confidence: 99%

Retymicin, Galtamycin B, Saquayamycin Z and Ribofuranosyllumichrome, Novel Secondary Metabolites from Micromonospora sp. Tü 6368

et al. 2005

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“…Morphological observations were made on plates containing oatmeal agar, thin potato-carrot agar, water agar, and soil extract agar (43). The additional media used to characterize strain SK&F-AAD-216T were inorganic salts-starch agar (44), peptone-yeast extract-iron agar (44), starch-casein-nitrate agar (22), DNase Test Agar (BBL Microbiology Systems), Bennett agar (17), tyrosine agar (44), gelatin medium 19 (49), thin Pablum agar (31), and litmus milk medium (Difco Laboratories). All tests were performed at * Corresponding author.…”

Section: Methodsmentioning

confidence: 99%

New Genus of the Actinomycetales: Kibdelosporangium aridum gen. nov., sp. nov.

Shearer¹,

Colman²,

Ferrin³

et al. 1986

International Journal of Systematic Bacteriology

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“…After grinding lichen samples with mortar and pestle, a homogenate was prepared using sterile 0.85% NaCl in a 1:10 (w/v) ratio, together with a lab stomacher (BagMixer; Interscience, St Nom, France). Diluted fractions were plated on R2A agar (Carl Roth, Karlsruhe, Germany), R2A agar with 25 mg ml À 1 cycloheximide, starch casein agar (Kuester and Williams, 1964) and ISP2 agar (Shirling and Gottlieb, 1966). Bacterial colonies were randomly picked within 5 days of incubation at room temperature.…”

Section: Isolation Of Lichen-associated Bacteriamentioning

confidence: 99%

Exploring functional contexts of symbiotic sustain within lichen-associated bacteria by comparative omics

et al. 2014

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Symbioses represent a frequent and successful lifestyle on earth and lichens are one of their classic examples. Recently, bacterial communities were identified as stable, specific and structurally integrated partners of the lichen symbiosis, but their role has remained largely elusive in comparison to the well-known functions of the fungal and algal partners. We have explored the metabolic potentials of the microbiome using the lung lichen Lobaria pulmonaria as the model. Metagenomic and proteomic data were comparatively assessed and visualized by Voronoi treemaps. The study was complemented with molecular, microscopic and physiological assays. We have found that more than 800 bacterial species have the ability to contribute multiple aspects to the symbiotic system, including essential functions such as (i) nutrient supply, especially nitrogen, phosphorous and sulfur, (ii) resistance against biotic stress factors (that is, pathogen defense), (iii) resistance against abiotic factors, (iv) support of photosynthesis by provision of vitamin B 12 , (v) fungal and algal growth support by provision of hormones, (vi) detoxification of metabolites, and (vii) degradation of older parts of the lichen thallus. Our findings showed the potential of lichenassociated bacteria to interact with the fungal as well as algal partner to support health, growth and fitness of their hosts. We developed a model of the symbiosis depicting the functional multi-player network of the participants, and argue that the strategy of functional diversification in lichens supports the longevity and persistence of lichens under extreme and changing ecological conditions.

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Selection of Media for Isolation of Streptomycetes

Cited by 776 publications

References 6 publications

Retymicin, Galtamycin B, Saquayamycin Z and Ribofuranosyllumichrome, Novel Secondary Metabolites from Micromonospora sp. Tü 6368

Retymicin, Galtamycin B, Saquayamycin Z and Ribofuranosyllumichrome, Novel Secondary Metabolites from Micromonospora sp. Tü 6368

New Genus of the Actinomycetales: Kibdelosporangium aridum gen. nov., sp. nov.

Exploring functional contexts of symbiotic sustain within lichen-associated bacteria by comparative omics

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