Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterization

Babot, Jaime Daniel; Argañaraz-Martínez, Eloy; Saavedra, Lucila; Apella, María C.; Chaia, Adriana Pérez

doi:10.1016/j.rvsc.2014.06.001

Cited by 23 publications

(21 citation statements)

References 46 publications

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“…Adhesion assays were performed according to Babot et al (2014) with some modifications. Briefly, animals were sacrificed by cervical dislocation and immediately eviscerated for collection of ileum, which was in turn rinsed repeatedly with ice-cold PBS pH 7 to eliminate the digesta content.…”

Section: Ex Vivo Bacterial Adhesion To Intestinal Tissue Fragmentsmentioning

confidence: 99%

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Feruloyl esterase activity is influenced by bile, probiotic intestinal adhesion and milk fat

Mukdsi

Argañaraz-Martínez

Chaia

et al. 2016

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Cinnamoyl esterases (CE) are microbial and mammalian intestinal enzymes able to release antioxidant hydroxycinnamic acids from their non-digestible ester-linked forms naturally present in vegetable foods. Previous findings showed that oral administration of Lactobacillus fermentum CRL1446 increased intestinal CE activity and improved oxidative status in mice. The aim of this work was to evaluate the in vitro CE activity of L. fermentum CRL1446 and the effect of bile on this activity, as well as strain resistance to simulated gastrointestinal tract (GIT) conditions and its ability to adhere to intestinal epithelium and influence its basal CE activity. L. fermentum CRL1446 and L. fermentum ATCC14932 (positive control for CE activity) were able to hydrolyse different synthetic hydroxycinnamates, with higher specificity toward methyl ferulate (3,853.73 and 899.19 U/g, respectively). Feruloyl esterase (FE) activity was mainly intracellular in L. fermentum CRL1446 and cell-surface associated in L. fermentum ATCC14932. Both strains tolerated simulated GIT conditions and were able to adhere ex vivo to intestinal epithelium. Pre-incubation of L. fermentum strains with bile increased FE activity in both whole cells and supernatants (~2-fold), compared to controls, suggesting that cells were permeabilised by bile, allowing more substrate to enter the cell and/or leakage of FE enzymes. Three-fold higher FE activities were detected in intestinal tissue fragments with adhered L. fermentum CRL1446 cells compared to control fragments (without bacteria), indicating that this strain provides exogenous FE activity and could stimulate esterase activity in the intestinal mucosa. Finally, we found that milk fat had a negative effect on FE activity of intestinal tissue, in absence or presence of adhered L. fermentum. These results help explaining the increase in intestinal FE activity previously observed in mice fed with L. fermentum CRL1446, and support the potential use of this strain for the development of new functional foods directed to oxidative stress-related ailments.

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Section: Ex Vivo Bacterial Adhesion To Intestinal Tissue Fragmentsmentioning

confidence: 99%

“…Adhesion results were admissible when viable cell counts in controls were negative or lower than 10 1 cfu/mm 2 . According to Babot et al (2014), strains were classified as: adherent (>1×10 3 cfu/mm 2 ); weakly adherent (10 1 -10 3 cfu/mm 2 ); and non-adherent (<1×10 1 cfu/mm 2 ).…”

Section: Ex Vivo Bacterial Adhesion To Intestinal Tissue Fragmentsmentioning

confidence: 99%

Feruloyl esterase activity is influenced by bile, probiotic intestinal adhesion and milk fat

Mukdsi

Argañaraz-Martínez

Chaia

et al. 2016

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“…Antibiotics susceptibility assay were performed according to the method described by Babot et al (2014) and modified by well diffusion agar method. Culture of 14 h of LAB isolates was poured (10 8 -10 9 CFU/mL) into MRS agar plate.…”

Section: Susceptibility To Antibioticsmentioning

confidence: 99%

“…The adhesion ability of E8 isolate was better than those of E5 and E7 isolates, which was indicated by the number of viable bacteria was 9.40±0.00 Log CFU/cm 2 with adhesion cell was 94.77±0.09%, while E5 isolate had adhesion ability that was too low i.e., 81.81±1.46%. Babot et al (2014) reported that two strains isolated from broiler's intestine had different adhesion abilities, L. reuteri LET 206 was able to adhere to chicken ileal cell as much as 43.3% while L. reuteri LET 211 was able to adhere to ileal cell as much as 37%. The differences in adhesion abilities of strains are specific and depend on physiology of cell and composition of cell wall (Ranadheera et al, 2012).…”

Section: Ability Of Lab Isolates To Adhere To Chicken Ileal Cellmentioning

confidence: 99%

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Selection of Lactic Acid Bacteria as Probiotic Candidate for Chicken

Hamida

Wiryawan

Meryandini

2015

Med Pet

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Lactic acid bacteria (LAB) regarded as safe microorganisms; they can naturally live in gastrointestinal tract, so appropriately used as a probiotic for chicken. This study aimed to select six isolates of LAB (E1223, E3, E4, E5, E7, and E8) to obtain the isolates potentially as probiotic candidate for chicken. The six isolates were derived from spontaneous fermented corn obtained from Laboratory of Animal Biotechnology and Biomedical, PPSHB, Bogor Agricultural University, Indonesia. LAB isolates were tested their susceptibility to antibiotics (bambermycin, erythromycin, chloramphenicol, and tetracycline) then were examined in vitro for their tolerance to gastrointestinal pH (2, 3, 4, and 7.2) and 0.5% bile salt condition, antimicrobial activity against Salmonella enteritidis and Enterococcus casseliflavus, and ability to adhere to chicken ileal cells. The results showed the isolates E5, E7, and E8 were sensitive to tetracycline and chloramphenicol, they could survive at pH 2, 3, 4, and 7.2, could survive at 0.5% bile salts, produced antimicrobial activity, and able to adhere to ileal cells (9.40±0.00 Log CFU/cm 2 of E8) and were significantly (P<0.05) higher than those of control (5.30±0.14 Log CFU/cm 2 ). In conclusion, this study showed that isolate E8 had better potential compared to isolates E5 and E7 in most in vitro assays as a probiotic candidate for chicken. E5, E7, and E8 were closely related with Pediococcus pentosaceus based on 16S rRNA gene. Key words: LAB, probiotic, chicken, in vitro ABSTRAKBakteri asam laktat (BAL) dianggap sebagai mikroorganisme aman, dapat hidup di dalam saluran pencernaan, sehingga tepat digunakan sebagai mikroorganisme probiotik untuk ayam. Penelitian ini bertujuan menyeleksi secara in vitro enam isolat BAL (E1223, E3, E4, E5, E7, E8) untuk memperoleh BAL berpotensi sebagai kandidat probiotik ayam. Enam isolat berasal dari jagung fermentasi yang diperoleh dari Laboratorium Bioteknologi Hewan dan Biomedis, PPSHB, Institut Pertanian Bogor, Indonesia. Isolat BAL diuji sensitifitasnya terhadap antibiotik (bambermisin, eritromisin, kloramfenikol, dan tetrasiklin) lalu diuji secara in vitro ketahanannya terhadap pH saluran pencernaan (2; 3; 4; dan 7,2) dan 0,5% garam empedu, aktivitas antimikroba terhadap Salmonella enteritidis dan Enterococcus casseliflavus, dan kemampuan menempel pada ileum ayam. Hasil penelitian menunjukkan bahwa isolat E5, E7, dan E8 sensitif terhadap tetrasiklin dan kloramfenikol, toleran pada pH pencernaan dan garam empedu, menghasilkan aktivitas antimikrob, dan mampu menempel pada ileum ayam (sel isolat E8 yang menempel sebesar 9,40±0,00 Log CFU/cm 2 ) signifikan (P<0,05) lebih tinggi dibandingkan dengan kontrol (5,30±0,14 Log CFU/cm 2 ). Kesimpulan penelitian ini menunjukkan bahwa isolat E8 memiliki potensi lebih baik daripada isolat E5 dan E7 di sebagian besar uji in vitro sebagai kandidat probiotik ayam. Isolat E5, E7, dan E8 memiliki homologi 99% dengan Pediococcus pentosaceus berdasarkan gen 16S rRNA.

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Microencapsulation of Probiotics with Soy Protein Isolate and Alginate for the Poultry Industry

Babot

Argañaraz-Martínez

Apella

et al. 2023

Food Bioprocess Technol

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Many probiotic products, with properly selected microorganisms, may not be effective for the intended purpose due to the low tolerance of microorganisms to gastrointestinal digestion. The microencapsulation seems to be one of the most promising techniques to protect probiotics against adverse environmental conditions. Therefore, the aim of this work was the design of soy protein isolate-alginate microcapsules for the encapsulation of probiotics for the poultry industry by the water-in-oil emulsion technique. To this end, the strain Ligilactobacillus salivarius CRL2217, with the ability to bind wheat germ agglutinin (WGA) on its surface and protect intestinal epithelial cells from the cytotoxicity of the glycoprotein, was used as model microorganism. Several parameters were varied in order to find the better conditions for microencapsulation: oil source and nature, SPI and sodium alginate concentration, stirring equipment and time for emulsion formation, CaCl 2 concentration, and absence or presence of stirring after the addition of the CaCl 2 solution. The survival of entrapped cells to a simulated gastric digestion and their survival and release during simulated intestinal digestion were also investigated. The obtained particles effectively protected L. salivarius CRL2217 from the proteolytic activity and low pH present in the gastric environment. Besides, their content was released in contact with a simulated intestinal juice, as viable counts and binding of WGA after a simulated intestinal digestion revealed. This work paves the way for the design of probiotic supplements for poultry including gastrointestinal digestion-susceptible bacteria.

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Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterization

Cited by 23 publications

References 46 publications

Feruloyl esterase activity is influenced by bile, probiotic intestinal adhesion and milk fat

Feruloyl esterase activity is influenced by bile, probiotic intestinal adhesion and milk fat

Selection of Lactic Acid Bacteria as Probiotic Candidate for Chicken

Microencapsulation of Probiotics with Soy Protein Isolate and Alginate for the Poultry Industry

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