Selection of<i>Bacillus subtilis</i>US191 as a mannanase‐producing probiotic candidate

Blibech, Monia; Mouelhi, Sana; Farhat-Khemakhem, Ameny; Boukhris, Ines; Ayeb, Afef El; Chouayekh, Hichem

doi:10.1002/bab.1798

Cited by 9 publications

(2 citation statements)

References 43 publications

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“…However, the unintended negative effects of feeding higher levels of yeast and yeast bioactives are possible. For example, the yeast cell wall mannans that have been found to be antinutritional ( Blibech et al, 2019 ; Chen et al, 2021 ). Over production of immune proteins and stimulation of lymphoid organs require nutrients that would have otherwise supported growth.…”

Section: Discussionmentioning

confidence: 99%

Enzymatically treated yeast bolstered growth performance of broiler chicks from young broiler breeders linked to improved indices of intestinal function, integrity, and immunity

Maina¹,

Thanabalan²,

Gasarabwe³

et al. 2022

Poultry Science

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Section: Discussionmentioning

confidence: 99%

Enzymatically treated yeast bolstered growth performance of broiler chicks from young broiler breeders linked to improved indices of intestinal function, integrity, and immunity

Maina¹,

Thanabalan²,

Gasarabwe³

et al. 2022

Poultry Science

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“…Probiotics are live microorganisms that exert beneficial effects on the host [36][37][38]. Evidence suggests the use of probiotics as a promise approach for promoting the wound healing process via inducing the immunity system and controlling inflammation.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus bulgaricus and Lactobacillus plantarum improve diabetic wound healing through modulating inflammatory factors

Mohtashami

Mohamadi

Azimi‐Nezhad

et al. 2020

Biotechnology and Applied Biochemistry

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Probiotics are nonpathogenic bacterial strains that exert beneficial effects on the host. Previous studies have shown that topical use of some strains of probiotic bacteria have good effects on the healing of cutaneous wounds. In the current study, the wound healing potentials of bacterial probiotics on diabetic cutaneous wounds were evaluated. The effects of probiotics on migration, the viability of fibroblasts, and macrophage proliferation were measured through using wound healing assay, methylthiazol tetrazolium assay, and bromodeoxyuridine, respectively. In this regard, in vivo diabetic wound healing experiments in Wistar rats following treatment with nontoxic concentrations of Lactobacillus bulgaricus and Lactobacillus plantarum were conducted. The histopathological and gene expression analyses were performed following removal of wound sites 3, 7, and 14 days postwounding. Results showed that treatment with probiotics accelerated the healing process of diabetic wounds and modulated the inflammatory cells in wound sites during a 14-day period postwounding. The altered mRNA levels of inflammatory cytokines were observed in wound sites following treatment with probiotics. The findings of the current study reveal that L. bulgaricus and L. plantarum could improve the healing of diabetic wounds via regulation of inflammation.

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Optimization of β‐mannanase production by Bacillus subtilis US191 using economical agricultural substrates

Blibech

Farhat‐Khemakhem

Kriaa

et al. 2020

Biotechnology Progress

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The Bacillus subtilis US191 strain producing highly thermostable β‐mannanase was previously selected as potential probiotic candidate for application as feed supplement in poultry industry. Initially, the level of extracellular β‐mannanase production by this strain was 1.48 U ml−1. To improve this enzyme titer, the present study was undertaken to optimize the fermentation conditions through experimental designs and valorization of agro‐industrial byproducts. Using the Plackett–Burman design, in submerged fermentation, a set of 14 culture variables was evaluated in terms of their effects on β‐mannanase production. Locust bean gum (LBG), soymeal, temperature, and inoculum size were subsequently optimized by response surface methodology using Box–Behnken design. Under optimized conditions (1 g L−1 LBG, 8 g L−1 soymeal, temperature of 30°C and inoculum size of 1010 CFU ml−1), a 2.59‐fold enhancement in β‐mannanase titer was achieved. Next, to decrease the enzyme production cost, the effect of partial substitution of LBG (1 g L−1) by agro‐industrial byproducts was investigated, and a Taguchi design was applied. This allowed the attaining of a β‐mannanase production level of 8.75 U ml−1 in presence of 0.25 g L−1 LBG, 5 g L−1 of coffee residue powder, 5 g L−1 of date seeds powder, and 5 g L−1 of prickly pear seeds powder as mannans sources. Overall, a 5.91‐fold improvement in β‐mannanase production by B. subtilis US191 was achieved.

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Selection ofBacillus subtilisUS191 as a mannanase‐producing probiotic candidate

Cited by 9 publications

References 43 publications

Enzymatically treated yeast bolstered growth performance of broiler chicks from young broiler breeders linked to improved indices of intestinal function, integrity, and immunity

Enzymatically treated yeast bolstered growth performance of broiler chicks from young broiler breeders linked to improved indices of intestinal function, integrity, and immunity

Lactobacillus bulgaricus and Lactobacillus plantarum improve diabetic wound healing through modulating inflammatory factors

Optimization of β‐mannanase production by Bacillus subtilis US191 using economical agricultural substrates

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