Selection of highly specific aptamers to Vibrio parahaemolyticus using cell-SELEX powered by functionalized graphene oxide and rolling circle amplification

Song, Shixi; Wang, Xingyu; Xu, Ke; Li, Qiao; Ning, Lufang; Yang, Xingbin

doi:10.1016/j.aca.2018.11.047

Cited by 36 publications

(28 citation statements)

References 51 publications

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“…These multivalent aptamers can also be incorporated with reduced graphene oxide, magnetic beads, FRET (fluorescent resonance energy transfer)‐based fluorescence molecules, or ferrocene (Figure C) for enhanced drug loading, efficient drug‐releasing or multiplexed cell imaging. Furthermore, RCA‐based multivalent aptamers could be incorporated with fluorophore‐labeled oligonucleotide or HRP for biosensors, used to prepare degradable aptamer hydrogel for cancer immunotherapy, or as an amplification strategy for aptamer SELEX . Owing to their enhanced binding affinity, size‐tunable property, exceptionally resistant ability to exonuclease degradation, and capability to circumvent multidrug resistance, multivalent aptamers from RCA have great potential for practical biomedical application.…”

Section: Rca With Functional Nucleic Acidsmentioning

confidence: 99%

Circular Nucleic Acids: Discovery, Functions and Applications

Mohammed-Elsabagh

Paczkowski

et al. 2020

ChemBioChem

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acids (CNAs) are nucleic acid molecules with a closed-loop structure. This feature comes with a number of advantages including complete resistance to exonuclease degradation, much better thermodynamic stability, and the capability of being replicated by a DNA polymerase in a rolling circle manner. Circular functional nucleic acids, CNAs containing at least a ribozyme/DNAzyme or a DNA/RNA aptamer, not only inherit the advantages of CNAs but also offer some unique application opportunities, such as the design of topologycontrolled or enabled molecular devices. This article will begin by summarizing the discovery, biogenesis, and applications of naturally occurring CNAs, followed by discussing the methods for constructing artificial CNAs. The exploitation of circular functional nucleic acids for applications in nanodevice engineering, biosensing, and drug delivery will be reviewed next. Finally, the efforts to couple functional nucleic acids with rolling circle amplification for ultra-sensitive biosensing and for synthesizing multivalent molecular scaffolds for unique applications in biosensing and drug delivery will be recapitulated.[a] Dr.

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Section: Rca With Functional Nucleic Acidsmentioning

confidence: 99%

Circular Nucleic Acids: Discovery, Functions and Applications

Mohammed-Elsabagh

Paczkowski

et al. 2020

ChemBioChem

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“…Vibrio parahemolyticus is a common foodborne pathogen. Song et al utilized a modified graphene oxide SELEX, coupled with isothermal rolling circle amplification to identify an aptamer specific for V. parahemolyticus (Vibrio parahemolyticus) [ 22 ]. The best aptamer had a reported Kd of 10.3 ± 2.5 nM.…”

Section: In Vitro Selection Of Ssdna Aptamersmentioning

confidence: 99%

Recent Progress in the Identification of Aptamers Against Bacterial Origins and Their Diagnostic Applications

Trunzo

Hong

2020

IJMS

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Aptamers have gained an increasing role as the molecular recognition element (MRE) in diagnostic assay development, since their first conception thirty years ago. The process to screen for nucleic acid-based binding elements (aptamers) was first described in 1990 by the Gold Laboratory. In the last three decades, many aptamers have been identified for a wide array of targets. In particular, the number of reports on investigating single-stranded DNA (ssDNA) aptamer applications in biosensing and diagnostic platforms have increased significantly in recent years. This review article summarizes the recent (2015 to 2020) progress of ssDNA aptamer research on bacteria, proteins, and lipids of bacterial origins that have implications for human infections. The basic process of aptamer selection, the principles of aptamer-based biosensors, and future perspectives will also be discussed.

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“…The use of these rapid and cheap POC tests could be used to determine environmental sources of infection and the effectiveness of current hospital cleaning practices. Aptamers and antibodies are both excellent choices for recognition molecules [56,63,128], however, given that aptamers are more stable at varying temperatures and pH levels, and have been shown to be more sensitive than many antibodies, they would be a better choice moving forward. Not only has the influenza assay shown that aptamers can be more sensitive [56], but they are more easily modifiable during selection and can easily be generated to specifically target multiple bacteria with a single aptamer [87], allowing for cheaper and easier POC systems.…”

Section: Detection Systemsmentioning

confidence: 99%

Novel Detection of Nasty Bugs, Prevention Is Better than Cure

Strom

Crowley

Shigdar

2020

IJMS

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Hospital-acquired infections (HAIs) are a growing concern around the world. They contribute to increasing mortality and morbidity rates and are an economic threat. All hospital patients have the potential to contract an HAI, but those with weakened or inferior immune systems are at highest risk. Most hospital patients will contract at least one HAI, but many will contract multiple ones. Bacteria are the most common cause of HAIs and contribute to 80–90% of all HAIs, with Staphylococcus aureus, Clostridium difficile, Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumoniae accounting for the majority. Each of these bacteria are highly resistant to antibiotics and can produce a protective film, known as a biofilm, to further prevent their eradication. It has been shown that by detecting and eradicating bacteria in the environment, infection rates can be reduced. The current methods for detecting bacteria are time consuming, non-specific, and prone to false negatives or false positives. Aptamer-based biosensors have demonstrated specific, time-efficient and simple detection, highlighting the likelihood that they could be used in a similar way to detect HAI-causing bacteria.

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Selection of highly specific aptamers to Vibrio parahaemolyticus using cell-SELEX powered by functionalized graphene oxide and rolling circle amplification

Cited by 36 publications

References 51 publications

Circular Nucleic Acids: Discovery, Functions and Applications

Circular Nucleic Acids: Discovery, Functions and Applications

Recent Progress in the Identification of Aptamers Against Bacterial Origins and Their Diagnostic Applications

Novel Detection of Nasty Bugs, Prevention Is Better than Cure

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