Selection of enzyme preparations and temperature-time regimes of water-heat and enzymatic treatment in the development of complex technology of processing of grain raw materials

Зуева, Н. В.; Агафонов, Г. В.; Korchagina, Marina; Долгов, А. Н.; Чусова, А. Е.

doi:10.20914/2310-1202-2019-1-112-119

Cited by 14 publications

(5 citation statements)

References 5 publications

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“…В результате проведенных исследований показано, что подобранный мультиэнзимный комплекс обеспечивает глубокий гидролиз высокомолекулярных полимеров зернового сырья: полисахаридов -с образованием моно-и дисахаров; белков -с накоплением аминного азота и свободных аминокислот; фитиновых веществс высвобождением фосфатов в биодоступной форме, и позволяет получить концентрированное сусло с содержанием РСВ более 30%, что в 1,5 раза превышало концентрацию сусла, приготовленного по классической технологии и содержащего 18-22% РСВ [2,3,17].…”

Section: заключениеunclassified

“…2021;11(3):384-392. (In Russian) https://doi.org/10.21285/2227-2925-2021-11-3-384-392 ВВЕДЕНИЕ Создание ресурсосберегающих биотехнологий производства спирта предусматривает прежде всего разработку эффективных способов подготовки зернового сырья для получения концентрированного сусла [1][2][3][4].…”

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Selecting multi-enzyme composition and preparation conditions for strong wort

Серба

Оверченко

Игнатова

et al. 2021

Izvestiâ vuzov. Prikladnaâ himiâ i biotehnologiâ

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Development of sustainable biotechnologies for deep processing of grain raw materials requires effective mechanisms of obtaining strong wort for alcohol production. To provide qualitative characteristics of biochemical composition and rheological properties of strong wort, it is necessary to select optimal enzyme systems and conditions for deep conversion of high-molecular weight polymers of the grain. Previous research has proven the efficiency of carbohydrases for processing grain raw materials. However, there is little evidence on the catalytic effect of phytase, including in combination with other hydrolytic enzymes, on the degree of hydrolysis of polymers in grain raw materials when preparing strong wort. This paper demonstrates the effect of proteases and phytases in a multi-enzyme composition, as well as the conditions of enzymatic processing of raw materials, on the rheological and biochemical parameters of strong wort. Wheat, rye and corn were investigated. The synergism of the combined effect of studied hydrolases, including phytolytic and proteolytic enzymes, contributed to an increase in polymer conversion in this grain raw material and the concentration of soluble dry substances of the wort by 1.5 times. Using the proteases and phytases in the multienzyme composition allowed the concentration of the following components in the wort to be increased: glucose – by 1.2–1.3 times; amine nitrogen – by 1.5–2.2 times; phosphorus ions – by 1.4–4.3 times. Additionally, in the wort samples, the content of amino acids in the free form increased by over 4 times. It is shown that the pretreatment of grain raw materials at a temperature of 80–90ºС for 6 hours and saccharification for 1–2 hours using a complete complex of enzymes containing α-amylase, glucoamylase, xylanase, protease and phytase, allows a strong wort with a dry matter content of over 30% to be obtained. Moreover, a significant decrease in viscosity was noted (particularly for rye wort – by 1.3–1.9 times). Our results confirm the essential role of enzymes exhibiting substrate specificity to protein and phytic polymers in grain raw materials.

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Section: заключениеunclassified

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Selecting multi-enzyme composition and preparation conditions for strong wort

Серба

Оверченко

Игнатова

et al. 2021

Izvestiâ vuzov. Prikladnaâ himiâ i biotehnologiâ

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“…Пшеничную муку в количестве смешивали с водой температурой 50 °С в соотношении 1,5:1 в тестомесителе и вносили ферментный препарат «Висколаза» и протеолитический ферментный препарат Протоферм FP с дозировкой 0,6 ед. ПС/г белка [1].…”

Section: результаты и обсуждениеunclassified

Analysis of the chemical composition and properties of the main and side products during the implementation of new technologies for producing ethanol

Зуева

Агафонов

Lukinova

et al. 2020

Vestn. Voronež. gos. univ. inž. tehnol.

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The article studied yeast composition isolated from the separation of the mash, bran separated in the grinding step, as well as a protein supplement obtained by mixing these two products. Crude fat content ranges from 4,7 to 6,2%, crude protein content is 38%, which is 20% lower than in the fodder yeast, but 14% higher than that of the bran. It is also found that the resulting protein product enriched in micro- and macro-elements, as well as readily hydrolyzable and water soluble carbohydrates. crude fiber content is 5,5%, which is almost 2,5 times lower than in the bran. The composition of the feed protein supplement obtained with the introduction at the stage of aqueous suspension of wheat powdery cellulolytic enzyme preparation Viskolaza 150 L and without it. The enzyme preparation was added in the step of preparing aqueous suspensions and wheat powdery dosage of 0,01% by weight of the feedstock. protein feed additives obtained with and without addition of cellulolytic enzyme preparation of high quality crude protein content and protein. Thus, the content of crude protein in the protein with the introduction of an additive in an aqueous suspension of powdery Viskolazy 150 L was 37% whereas 34% without the enzyme preparation. The amount of soluble polysaccharides and the mass fraction of ash were practically at the same level of from 2,4 to 5% and from 5,5 to 7,0%, respectively. fiber content of the protein supplement using Viskolazy 150 L was 4,2-6,1%, which is 2,5 times lower than in the protein supplement obtained without enzyme preparation.

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“…The usage of this type of raw could enrich feed with macro-and microelements, vitamins and biologically valuable substances. The enrichment of feed increases its nutritional and biological value [1,2].…”

Section: Introductionmentioning

confidence: 99%

Development of the innovative biotechnological methods of the biological preparations production for feed purposes

Volkova

Соколова

Fursova

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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The aim of this study was the production of the biological preparations based on the hydrolyzed pea flour and the yeast biomass. The research objects were Saccharomyces cerevisiae Y-1218 yeast with glucoamylase activity, pea flour, and Celloviridin S20X and Amyloprotoorizin S20X enzyme preparations (EP). The specific growth rate of the yeast was determined by the calculation method, protein content – via Kjeldahl and Lowry methods, reducing substances content – by Somogyi-Nelson assay, the amine nitrogen content – by the formol titration method, the number of living yeast cells – by sowing of dilutions. The amino acid content was measured on the KNAUER (Germany) high-performance liquid chromatograph. As the result of the studies, enzyme preparations for the fermentative processing of pea flour were selected. The cultivation conditions of the Saccharomyces cerevisiae Y-1218 yeast were opted. Under these chosen growing parameters the produced biomass contained 2.4×109 CFU/cm3 and specific growth rate of yeast was 0.264 h-1. The resulting biomass contained a high amount of essential amino acids. The biopreparations production based on the described ingredient is perspective. These biopreparations with increased biological value could be used for feed purposes.

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Selection of enzyme preparations and temperature-time regimes of water-heat and enzymatic treatment in the development of complex technology of processing of grain raw materials

Cited by 14 publications

References 5 publications

Selecting multi-enzyme composition and preparation conditions for strong wort

Selecting multi-enzyme composition and preparation conditions for strong wort

Analysis of the chemical composition and properties of the main and side products during the implementation of new technologies for producing ethanol

Development of the innovative biotechnological methods of the biological preparations production for feed purposes

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