Selection of DNA aptamer against prostate specific antigen using a genetic algorithm and application to sensing

Savory, Nasa; Abe, Koichi; Sode, Koji; Ikebukuro, Kazunori

doi:10.1016/j.bios.2010.07.057

Cited by 152 publications

(95 citation statements)

References 27 publications

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“…Aptamers combined with electrochemical biosensor have been wildly applied in the detection of proteins, cancer cells, drugs, toxin and various small molecules [16][17][18][19][20]. In this work, a DNA aptamer for PSA [21] was utilized to develop a new electrochemical aptasensor for the measurement of human serum PSA.…”

Section: Introductionmentioning

confidence: 99%

Detection of the human prostate-specific antigen using an aptasensor with gold nanoparticles encapsulated by graphitized mesoporous carbon

Liu

Hua

et al. 2012

Microchim Acta

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We report on a sensitive electrochemical aptasensor for the detection of human prostate specific antigen (PSA). It is based on the signal amplification of the biotin-avidin system using a sensing platform that is making use of a graphite electrode modified with gold nanoparticles that were covered with graphitized mesoporous carbon nanoparticles (AuNPs@GMCs). The AuNPs@GMCs hybrid was prepared by linking 1,6-hexanedithiol-functionalized GMCs and gold nanoparticles via Au-S groups. Then, streptavidin was immobilized on the electrode modified with the AuNPs@GMCs so to enlarge the amount of biotin-aptamer which led to enhanced detection sensitivity. If an PSA aptamer captures the target PSA on the electrode, the differential pulse voltammetric (DPV) signal of the hexacyanoferrate redox system decreases. Factors affecting the performance of the aptasensor were studied in detail. Under optimal conditions, the DPV signal changes could be used to quantitatively detect PSA in the concentration range from 0.25 to 200 ng mL −1 , with a lowest limit of detection as small as 0.25 ng mL −1 . The aptasensor is highly specific and displays acceptable precision, good stability and repeatability.

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Section: Introductionmentioning

confidence: 99%

Detection of the human prostate-specific antigen using an aptasensor with gold nanoparticles encapsulated by graphitized mesoporous carbon

Liu

Hua

et al. 2012

Microchim Acta

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show abstract

“…The 5 0 -and 3 0 -termini of the aptamer sequences were modified with green fluorescence 6-carboxyfluorescein (FAM) and an amine group. The aptamer sequences were as follows: TBA (5 0 -GGTTGGTGTGGTTGG-3 0 ) [26], PSAA (5 0 -TTTAATTAAAGCTCTC-CATCAAATAGCTTTTTTTTTT-3 0 ) [27], and HAA (5 0 -GUCGGCA-UGCGGUA-3 0 ) [28]. Another TB-binding DNA aptamer, TBA29 (5 0 -AGTCCGTGGTAGGGCAGGTTGGGGTGACT-3 0 ) [29] was modified with red fluorescence TAMRA instead of FAM.…”

Section: Methodsmentioning

confidence: 99%

On-chip graphene oxide aptasensor for multiple protein detection

Ueno

Furukawa

Matsuo

et al. 2015

Analytica Chimica Acta

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“…Although in vitro selection is a powerful method to obtain aptamers, selected aptamers often require optimization for the best affinity and specificity. We can perform post-optimization of their sequences after SELEX through iterative cycles of mutated DNA synthesis and evaluation of their functions, which would be a cumbersome process for antibodies (Ikebukuro et al, 2005b;Ikebukuro et al, 2006;Knight et al, 2009;Noma et al, 2006b;Savory et al, 2010). The fifth advantage is important so that aptamers can be perceived as unique reagents for analytical applications, not as alternatives to antibodies.…”

Section: Introductionmentioning

confidence: 99%