Selection of Diazotrophic Bacterial Communities in Biological Sand Filter Mesocosms Used for the Treatment of Phenolic-Laden Wastewater

Ramond, Jean‐Baptiste; Welz, Pamela J.; Tuffin, Marla; Burton, Stephanie G.; Cowan, Don A.

doi:10.1007/s00248-013-0258-4

Cited by 7 publications

(4 citation statements)

References 31 publications

(45 reference statements)

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“…What is noteworthy from the NGS results is that the SWW was selective for the genus Geobacter and order Rhizobiales, confirming previous findings that organic-rich effluents select specific N-fixing community members in BSFs (Ramond et al, 2013). For efficient organic bioremediation in BSFs, the selected BSF microbial community must be able to both effectively degrade the 'contaminants' and counterbalance the high C:N ratio of the wastewater by fixing atmospheric N (Ramond et al, 2013). Members of the Geobacter genus and the Rhizobiales order (e.g.…”

Section: Qualitative and Quantitative Changes In The Nitrogen Fixing supporting

confidence: 88%

“…In a separate study, the nifH gene was used as a marker to monitor the changes in the NFB population in BSF systems exposed to a phenolic cocktail with a final COD concentration of 5842 mg/L and a high C:N ratio (100:0.1) (Ramond et al, 2013). Analysis of terminal restriction fragment length polymorphism (T-RFLP) and denaturing gradient gel electrophoresis (DGGE) results showed that there was a significant structural change in the NFB population after exposure to the phenolic cocktail, with the selection of various Azotobacter spp.…”

Section: Introductionmentioning

confidence: 99%

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Bacterial nitrogen fixation in sand bioreactors treating winery wastewater with a high carbon to nitrogen ratio

Welz

Ramond

Braun

et al. 2018

Journal of Environmental Management

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Heterotrophic bacteria proliferate in organic-rich environments and systems containing sufficient essential nutrients. Nitrogen, phosphorus and potassium are the nutrients required in the highest concentrations. The ratio of carbon to nitrogen is an important consideration for wastewater bioremediation because insufficient nitrogen may result in decreased treatment efficiency. It has been shown that during the treatment of effluent from the pulp and paper industry, bacterial nitrogen fixation can supplement the nitrogen requirements of suspended growth systems. This study was conducted using physicochemical analyses and culture-dependent and -independent techniques to ascertain whether nitrogen-fixing bacteria were selected in biological sand filters used to treat synthetic winery wastewater with a high carbon to nitrogen ratio (193:1). The systems performed well, with the influent COD of 1351 mg/L being reduced by 84-89%. It was shown that the nitrogen fixing bacterial population was influenced by the presence of synthetic winery effluent in the surface layers of the biological sand filters, but not in the deeper layers. It was hypothesised that this was due to the greater availability of atmospheric nitrogen at the surface. The numbers of culture-able nitrogen-fixing bacteria, including presumptive Azotobacter spp. exhibited 1-2 log increases at the surface. The results of this study confirm that nitrogen fixation is an important mechanism to be considered during treatment of high carbon to nitrogen wastewater. If biological treatment systems can be operated to stimulate this phenomenon, it may obviate the need for nitrogen addition.

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Section: Qualitative and Quantitative Changes In The Nitrogen Fixing supporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Bacterial nitrogen fixation in sand bioreactors treating winery wastewater with a high carbon to nitrogen ratio

Welz

Ramond

Braun

et al. 2018

Journal of Environmental Management

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“…For the DGGE analysis, total DNA was extracted from the digesta and mucosal samples with a TIANamp Stool DNA Kit (Tiangen, Beijing, China). The variable V3 region of 16S rRNA was amplified with PCR using the universal primers 341F (5′-CCTACGGGAGGCAGCAG-3′) and 534R (5′-ATTACCGCGGCTGCTGG-3′), and a GC clamp (5′-CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGG-3′) was attached to the 5′-terminus of 341F 47 48 . The PCR products were loaded onto 8% polyacrylamide gels containing 37.5:1 acrylamide:bisacrylamide and a denaturing gradient of 38–51% (100% denaturant was equivalent to 7 M urea and 40% deionised formamide) using the DCode Universal Mutation Detection System (Bio-Rad Laboratories, Hercules, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Use of recombinant porcine β-defensin 2 as a medicated feed additive for weaned piglets

Peng

Wang²,

Xie³

et al. 2016

Sci Rep

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Post-weaning diarrhoea (PWD) in piglets is associated with colonization of the intestine with bacterial pathogens. In this study, we evaluated the use of recombinant porcine β-defensin 2 (rpBD2) as a medicated feed additive for weaned piglets. The crude extract from the culture supernatant of rpBD2-expressing Pichia pastoris was used as a medicated feed additive for weaned piglets. Dietary treatments included a positive control (basal diet + antibiotics, designated PC) and three different rpBD2 treatments without antibiotics (basal diet supplemented with 1, 5, or 15 g of crude rpBD2/kg basal diet, designated 1PD, 5PD, and 15PD, respectively). Of all the treatments, 5PD had the greatest impact on the weaned piglets. It increased their body weight, average daily weight gain, average daily feed intake, and intestinal villus height in the duodenum and jejunum, and reduced the incidence of PWD. The diversity of the cecal digesta and mucosa microflora was compared between the weaned piglets in the PC and 5PD groups. Piglets treated with 5PD had lower diversity indices and fewer bacterial pathogens in their cecal digesta and mucosa than the PC group. Our results demonstrate that crude rpBD2 could provide an alternative to the traditional antibiotic feed additives given to weaned piglets.

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“…The composition and function of microbial communities in BSRs are influenced by the type of WW being treated, climatic conditions, the physicochemical properties of the sand and the WW chemistry and redox status of spatial niches within BSR [9,55,84]. Examples of the character of the influent on the microbial composition include the selection of biological sulfate reducing bacteria [9] and diazotrophic bacteria [85,86] in BSRs treating acid mine drainage and high C to nitrogen (N) WW, respectively. In BSRs that are not overloaded, nutrient concentrations and hence microbial biomass typically decrease with depth in VF systems and depth and length in HSSF systems [55,72,87].…”

Section: Selection and Acclimation Of Microbial Communitiesmentioning

confidence: 99%

Biosand Reactors for Municipal and Industrial Wastewater Treatment: Status Quo, Challenges and Opportunities

Welz

2024

Processes

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Biosand reactors (BSRs), alternatively known as slow or biological sand filters, are passive systems that are used to remove contaminants from domestic wastewater, industrial wastewater and drinking water. This review focuses specifically on their application for remediation of industrial effluent and sewage-containing municipal and household effluent. The relationships between the physicochemical characteristics of the sand grains (size, size distribution, shape, chemical composition) and the hydraulic conductivity of the sand employed in BSFs are critically discussed in relationship to the achievable loading rates and hydraulic retention times. The modes of operation and influence of the functional microbial biomass as well as biodegradable and recalcitrant particulates on these parameters is comprehensively reviewed. Finally, the bioremediation of sewage-based and industrial wastewater is examined. This includes an account of the biotic and abiotic removal mechanisms and the limitations of BSRs for removal of pollutants such as phosphorus/phosphate and nitrates/nitrites. The removal mechanisms and removal efficiencies of macronutrients, micropollutants, fecal indicators and other microorganisms such as antibiotic-resistant bacteria in BSRs are discussed.

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Selection of Diazotrophic Bacterial Communities in Biological Sand Filter Mesocosms Used for the Treatment of Phenolic-Laden Wastewater

Cited by 7 publications

References 31 publications

Bacterial nitrogen fixation in sand bioreactors treating winery wastewater with a high carbon to nitrogen ratio

Bacterial nitrogen fixation in sand bioreactors treating winery wastewater with a high carbon to nitrogen ratio

Use of recombinant porcine β-defensin 2 as a medicated feed additive for weaned piglets

Biosand Reactors for Municipal and Industrial Wastewater Treatment: Status Quo, Challenges and Opportunities

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