Selection of column and gradient for the separation of polyphenols in sherry wine by high-performance liquid chromatography incorporating internal standards

Sánchez, Dominico Antonio Guillén; Barroso, Carmelo Garcı́a; Pérez-Bustamante, J.A.

doi:10.1016/0021-9673(95)00931-0

Cited by 33 publications

(11 citation statements)

References 8 publications

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“…After 16 h shake in 35 ºC water bath in the dark, the extract was centrifuged at 12000g for 20 min and the samples filtered through a 0.45 µm filter. Quantitative and qualitative analysis was done by reverse phase HPLC (Guillen et al, 1996). The HPLC apparatus consisted of a KNAUER (Germany), UV-Vis detector, and reverse-phase C 18 column at 25 ºC.…”

Section: Determination Of Pal Activitymentioning

confidence: 99%

Effects of Salinity on Phenolic Compounds in Tolerant and Sensitive Grapes

Mohammadkhani¹

2018

AgricultForest

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Salinity is a major environmental stress and grape is classified as a moderately sensitive plant to salinity. Plants have anti-oxidative systems involving antioxidant enzymes and secondary metabolites like phenolic compounds. The aim of this study was to examine the salinity induced alterations, PAL enzyme activity and total phenolics contents in four grape genotypes. In this study two tolerant (Gharashani and H6) and two sensitive (Shirazi and GhezelUzum) grapes-according to screening experiments under salinity-were selected. 50 mM NaCl was used for salinity treatment. Total phenolics content and phenylalanine ammonia-lyase (PAL) activity increased in all the genotypes under salinity. A positive significant correlation (P<0.01, r>0.8) was observed between total phenolics and PAL activity in leaves and roots of grapes. Ten phenolic acids including hydroxybenzoic acid and hydroxycinnamic acid derivatives were analyzed. Our tolerant and sensitive genotypes showed main differences in phenolic compounds accumulation under salinity. Some compounds like gallic, syringic and p-coumaric acids accumulated higher in tolerant genotypes and others like vanillic, caffeic and sinapic acids accumulated in sensitive genotypes. Considering our results it seems that syringic and p-coumaric acids-that was higher in leaves and roots of tolerant genotypeshad a key role in salinity tolerance.

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Section: Determination Of Pal Activitymentioning

confidence: 99%

Effects of Salinity on Phenolic Compounds in Tolerant and Sensitive Grapes

Mohammadkhani¹

2018

AgricultForest

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“…Qualitative and quantitative analysis was performed by reverse phase HPLC apparatus (Guillen et al, 1996) consisting of a Shimadu (Japan) LC-6A series pumping system, SIL-6A automatic injector furnished with a 50-mL loop, SPD-6AV UV-visible detector and C-R6A chromatography data station software. Separation of the compounds was carried out using a Merck LiChrospher 100RP-18e column (250 Â 4.0 mm ID, 5 mm) joined to a Merck RP-18 (10 mm Â 4.0 mm) guard column at 30 1C.…”

Section: Extraction and Determination Of Phenolic Acid Contentsmentioning

confidence: 99%

Changes and subcellular localizations of the enzymes involved in phenylpropanoid metabolism during grape berry development

Chen

Wen

Kong

et al. 2006

Journal of Plant Physiology

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“…HPLC analysis of proanthocyanidins has often been described in the literature. 17,19,[22][23][24][25][29][30][31][32][33][34][35] A good separation is obtained with a large excess of acid (formic acid or TFA), favoring the protonation of the hydroxyl group and thus decreasing interactions between the sample and the stationary phase. However, positive-ion electrospray ionization of proanthocyanidins generates low intensity ions (5 × 10 4 ), so that mass spectrometric analysis of the compounds is very difficult.…”

Section: Analysis Of Oligomeric Tanninsmentioning

confidence: 99%

Analysis of Oligomeric and Polymeric Tannins of Grape Berries by Liquid Chromatography/Electrospray Ionization Multiple-Stage Tandem Mass Spectrometry

Perret

Tabacchi

Pezet

2001

Eur J Mass Spectrom (Chichester)

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Tannins are highly polar, water-soluble and thermally-labile compounds which are difficult to ionize. Mass spectrometry is very useful for the structural determination of such compounds. In this article, we describe a sensitive method for the identification of tannic composition in grape berries (var. Gamaret). An ion trap, equipped with an electrospray ionization (ESI) interface, allowed us to develop multiple-stage fragmentation (MS n), which provided useful structural information. This technique was applied to a mixture of proanthocyanidins and proanthocyanidin gallate oligomers (B-type) generating identical ion sequences for each parent ion.

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Selection of column and gradient for the separation of polyphenols in sherry wine by high-performance liquid chromatography incorporating internal standards

Cited by 33 publications

References 8 publications

Effects of Salinity on Phenolic Compounds in Tolerant and Sensitive Grapes

Effects of Salinity on Phenolic Compounds in Tolerant and Sensitive Grapes

Changes and subcellular localizations of the enzymes involved in phenylpropanoid metabolism during grape berry development

Analysis of Oligomeric and Polymeric Tannins of Grape Berries by Liquid Chromatography/Electrospray Ionization Multiple-Stage Tandem Mass Spectrometry

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