Abstract:Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca.
Questions? Contact the NRC Publications Archive team atPublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the pub… Show more
“…Because the cells are isolated by their own migration, it is reasonable to believe that these cells are still active and suitable for follow-up applications. Similar to what has been discussed by Natarajan et al., this kind of cells selected by migration would have a better therapeutic efficacy [35]. Such a design is believed to be capable of sorting out the target cells while eliminating the senescent subjects.Figure 3Design of the dual gradients hydrogel scaffold.…”
Section: Hydrogels With Gradients For Stem Cell Sortingmentioning
confidence: 88%
“…Owing to cellular chemotaxis, cells are intended to move towards the side with higher biomolecule concentration. In a platform with epidermal growth factor (EGF) gradients, adipose-derived stem cells migrated to the high EGF concentration were sorted out as higher chemotaxis for EGFs [35]. In this study, the cells were primary cells isolated from rat subcutaneous adipose tissues.…”
Section: Hydrogels With Gradients For Stem Cell Sortingmentioning
Isolating active mesenchymal stem cells from a heterogeneous population is an essential step that determines the efficacy of stem cell therapy such as for osteoarthritis. Nowadays, the gold standard of cell sorting, fluorescence-activated cell sorting, relies on labelling surface markers via antibody–antigen reaction. However, sorting stem cells with high stemness usually requires the labelling of multiple biomarkers. Moreover, the labelling process is costly, and the high operating pressure is harmful to cell functionality and viability. Although label-free cell sorting, based on physical characteristics, has gained increasing interest in the past decades, it has not shown the ability to eliminate stem cells with low stemness. Cell motility, as a novel sorting marker, is hence proposed for label-free sorting active stem cells. Accumulating evidence has demonstrated the feasibility in manipulating directional cell migration through patterning the biophysical, biochemical or both gradients of the extracellular matrix. However, applying those findings to label-free cell sorting has not been well discussed and studied. This review thus first provides a brief overview about the effect of biophysical and biochemical gradients of the extracellular matrix on cell migration. State-of-the-art fabrication techniques for generating such gradients of hydrogels are then introduced. Among current research, the authors suggest that hydrogels with dual-gradients of biochemistry and biophysics are potential tools for accurate label-free cell sorting with satisfactory selectivity and efficiency.
Translational potential of this article
The reviewed label-free cell sorting approaches enable us to isolate active cell for cytotherapy. The proposed system can be further modified for single-cell analysis and drug screening.
“…Because the cells are isolated by their own migration, it is reasonable to believe that these cells are still active and suitable for follow-up applications. Similar to what has been discussed by Natarajan et al., this kind of cells selected by migration would have a better therapeutic efficacy [35]. Such a design is believed to be capable of sorting out the target cells while eliminating the senescent subjects.Figure 3Design of the dual gradients hydrogel scaffold.…”
Section: Hydrogels With Gradients For Stem Cell Sortingmentioning
confidence: 88%
“…Owing to cellular chemotaxis, cells are intended to move towards the side with higher biomolecule concentration. In a platform with epidermal growth factor (EGF) gradients, adipose-derived stem cells migrated to the high EGF concentration were sorted out as higher chemotaxis for EGFs [35]. In this study, the cells were primary cells isolated from rat subcutaneous adipose tissues.…”
Section: Hydrogels With Gradients For Stem Cell Sortingmentioning
Isolating active mesenchymal stem cells from a heterogeneous population is an essential step that determines the efficacy of stem cell therapy such as for osteoarthritis. Nowadays, the gold standard of cell sorting, fluorescence-activated cell sorting, relies on labelling surface markers via antibody–antigen reaction. However, sorting stem cells with high stemness usually requires the labelling of multiple biomarkers. Moreover, the labelling process is costly, and the high operating pressure is harmful to cell functionality and viability. Although label-free cell sorting, based on physical characteristics, has gained increasing interest in the past decades, it has not shown the ability to eliminate stem cells with low stemness. Cell motility, as a novel sorting marker, is hence proposed for label-free sorting active stem cells. Accumulating evidence has demonstrated the feasibility in manipulating directional cell migration through patterning the biophysical, biochemical or both gradients of the extracellular matrix. However, applying those findings to label-free cell sorting has not been well discussed and studied. This review thus first provides a brief overview about the effect of biophysical and biochemical gradients of the extracellular matrix on cell migration. State-of-the-art fabrication techniques for generating such gradients of hydrogels are then introduced. Among current research, the authors suggest that hydrogels with dual-gradients of biochemistry and biophysics are potential tools for accurate label-free cell sorting with satisfactory selectivity and efficiency.
Translational potential of this article
The reviewed label-free cell sorting approaches enable us to isolate active cell for cytotherapy. The proposed system can be further modified for single-cell analysis and drug screening.
“…In this regard, migration of rat adipose-derived stem cells to a well-defined gradient of EGF was carried out using a Y-shaped microfluidic device for the on-chip selection of cells with higher chemotactic ability. 46 The results of this study might be deployed to enhance stem cell homing to target organs and improve the cell therapy. Microfluidic devices can be used to create controllable and steady concentration gradients for quantitative evaluation of chemotaxis on neutrophils as well 47 as neutrophils migrate to specific locations to perform immune functions when sensing gradients of chemotactic factors.…”
Section: State Of the Art In Biological Applications Of Mammalian mentioning
Biomicrofluidics is an emerging field at the cross roads of microfluidics and life sciences which requires intensive research efforts in terms of introducing appropriate designs, production techniques, and analysis. The ultimate goal is to deliver innovative and cost-effective microfluidic devices to biotech, biomedical, and pharmaceutical industries. Therefore, creating an in-depth understanding of the transport phenomena of cells and biomolecules becomes vital and concurrently poses significant challenges. The present article outlines the recent advancements in diffusion phenomena of cells and biomolecules by highlighting transport principles from an engineering perspective, cell responses in microfluidic devices with emphases on diffusion- and flow-based microfluidic gradient platforms, macroscopic and microscopic approaches for investigating the diffusion phenomena of biomolecules, microfluidic platforms for the delivery of these molecules, as well as the state of the art in biological applications of mammalian cell responses and diffusion of biomolecules.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
