Selection of carrier for immobilization of fructosyltransferase from Aureobasidium pullulans

Abstract

The study deals with the development of an immobilized biocatalyst for the production of fructooligosaccharides. Several commercial anion-exchange resins and polymethacrylate carriers were tested for the immobilization either by the direct attachment of fructosyltransferase or by the attachment accompanied by simultaneous glutaraldehyde crosslinking in the pH range from 5.7 to 7.1. On the basis of the efficiency of immobilization and the storage stability, Amberlite IRA 900 and Dowex Marathon MSA were selected as the best carriers for fructosyltransferase immobilization by direct attachment at pH 5.7.

Biochemical characterization of immobilized tannase from Aspergillus awamori

“…Thus, further studies were performed on immobilized tannase on amberlite. Amberlite is considered to be better immobilization matrix for various other enzymes such as urease (Anita et al, 1997), lipase (Fazlena et al, 2006), and R-galactosidase (Onal and Telefoncu 2005), and fructosyltransferase (Platková et al, 2006).…”

Kinetics and modeling of fructo‐oligosaccharide synthesis by immobilized fructosyltransferase from Rhodotorula sp.

“…1 The β-frutofuranosidase enzymes, from different sources, have been immobilized in calcium alginate, 2,3 in glass or porous silica, 4,5 ion-exchange resins, 6,7 and in gluten. 8 The immobilized enzymes present some changes in their physical and chemical properties after the immobilization process, such as alteration in the enzymatic activity and kinetic stability and longer half-lives.…”

Amberlite IRA 900 versus calcium alginate in immobilization of a novel, engineered β‐fructofuranosidase for short‐chain fructooligosaccharide synthesis from sucrose

“…Several immobilization techniques and support materials have been developed in the quest to efficiently utilize the biocatalyst. These range from covalent immobilization on SHIRASU porous glass, chitosan particles, methacrylamide‐based polymeric beads and ceramic membranes to entrapment in calcium alginate (CA) beads, and adsorption onto anion‐exchange resins . A few of these have recorded impressive results on the laboratory scale, while others cannot be readily applied in food processing due to reservations about the use of potentially poisonous coupling agents like glutaraldehyde .…”

“…Amberlite IRA 900 (AI900) and Dowex Marathon MSA (DMM) are highly porous strong base anion exchange resins that have recorded satisfactory results in immobilizing FFase . They are both composed of a styrene–divinylbenzene matrix and have the advantage of being applicable in food processing, due to their nontoxic nature . However, the AI900 possesses trimethyl ammonium functional groups while the DMM has quaternary amine functional groups.…”