Selection of biochemical mutants of Aspergillus niger with enhanced catalase production

Fiedurek, J.; Gromada, A.

doi:10.1007/s002530050933

Cited by 23 publications

(19 citation statements)

References 6 publications

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“…One unit (U) of enzyme activity was defined as the amount of enzyme producing one micromole of hydrogen peroxide per minute at 30 °C. Extracellular and intracellular (measured as cell‐free extracts) catalase activity was measured spectrophotometrically by observing the decrease in light absorption at 525 nm during decomposition of H 2 O 2 by the enzyme ( Fiedurek and Gromada 1997). One unit (U) of catalase activity was defined as the amount of enzyme catalysing the decomposition of on micromole of hydrogen peroxide per minute at 30 °C.…”

Section: Methodsmentioning

confidence: 99%

Production of catalase and glucose oxidase by Aspergillus niger using unconventional oxygenation of culture

Fiedurek

Gromada

2000

J Appl Microbiol

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A novel method for increasing dissolved oxygen concentration in culture media has been developed. It involves adding hydrogen peroxide (H 2 O 2 ) to the medium which is then decomposed to oxygen and water by catalase. Some factors affecting oxygenation of culture were investigated. Maximal oxygen concentration occurred in 50 ml of the medium containing 0Á2 g wet mycelium and 0Á2% glucose at pH 5Á0. A new apparatus for automated addition of H 2 O 2 to the bioreactor to keep the dissolved oxygen concentration constant over the range 1±100% 2% was tested. A signi®cant increase (over sixfold) of intracellular catalase activity was obtained while the dissolved oxygen concentration remained stable (30% 2%).

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Section: Methodsmentioning

confidence: 99%

Production of catalase and glucose oxidase by Aspergillus niger using unconventional oxygenation of culture

Fiedurek

Gromada

2000

J Appl Microbiol

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“…On the other hand, SD at 0.02% has been sufficient for reduction of colonies of some species of Aspergillus (9,26).…”

Section: Discussionmentioning

confidence: 99%

Mutagenesis of Aspergillus oryzae IPT-301 to improve the production of β-fructofuranosidase

Maresma¹,

Castillo²,

Fernandez³

et al. 2010

Braz. J. Microbiol.

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Aspergillus oryzae IPT-301, previously reported as a β-fructofuranosidase producing microorganism, was successfully mutated using UV irradiation at 253.7 nm followed by the screening of survivors resistant to certain stress conditions. Strains were first subjected to the β-fructofuranosidase activity assay using a portion from the colony grown in Petri dish as the enzyme source. Seven mutants with β-fructofuranosidase activity values relative to the parent culture between 140 -190% were selected from survivors grown at temperature of 40ºC or 0.018% (w/v) sodium dodecyl sulfate concentration. They were cultivated on a rotary shaker to characterize mycelium and extracellular fructosyltransferase activities.Three mutants named IPT-745, IPT-746 and IPT-748 showed the highest amount of mycelium activity whose values increased 1.5 -1.8 fold, compared with the parent strain. It was found that more than 55% of total enzyme activity (mycelium-plus extracellular-activity) from these strains was detected in the mycelium fraction. Only one mutant, IPT-747, exceeded the amount of extracellular enzyme exhibited by the parent strain (1.5 times). This mutant also showed the highest value of total fructosyltransferase activity.

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“…The regular capacity of GOD in these organic frameworks is to act primarily as an antibacterial and antifungal specialist through the generation of H 2 O 2 . Among these sources, fungi have an eminent status and industrially fungal sources have been preferred since the early 1950s (Fiedurek and Gromada, 1997a). …”

Section: Overviewmentioning

confidence: 99%

Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current Updates

et al. 2017

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Fungal glucose oxidase (GOD) is widely employed in the different sectors of food industries for use in baking products, dry egg powder, beverages, and gluconic acid production. GOD also has several other novel applications in chemical, pharmaceutical, textile, and other biotechnological industries. The electrochemical suitability of GOD catalyzed reactions has enabled its successful use in bioelectronic devices, particularly biofuel cells, and biosensors. Other crucial aspects of GOD such as improved feeding efficiency in response to GOD supplemental diet, roles in antimicrobial activities, and enhancing pathogen defense response, thereby providing induced resistance in plants have also been reported. Moreover, the medical science, another emerging branch where GOD was recently reported to induce several apoptosis characteristics as well as cellular senescence by downregulating Klotho gene expression. These widespread applications of GOD have led to increased demand for more extensive research to improve its production, characterization, and enhanced stability to enable long term usages. Currently, GOD is mainly produced and purified from Aspergillus niger and Penicillium species, but the yield is relatively low and the purification process is troublesome. It is practical to build an excellent GOD-producing strain. Therefore, the present review describes innovative methods of enhancing fungal GOD production by using genetic and non-genetic approaches in-depth along with purification techniques. The review also highlights current research progress in the cost effective production of GOD, including key advances, potential applications and limitations. Therefore, there is an extensive need to commercialize these processes by developing and optimizing novel strategies for cost effective GOD production.

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Selection of biochemical mutants of Aspergillus niger with enhanced catalase production

Cited by 23 publications

References 6 publications

Production of catalase and glucose oxidase by Aspergillus niger using unconventional oxygenation of culture

Production of catalase and glucose oxidase by Aspergillus niger using unconventional oxygenation of culture

Mutagenesis of Aspergillus oryzae IPT-301 to improve the production of β-fructofuranosidase

Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current Updates

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