Selection of bacteria suitable for introduction into the rhizosphere of grass

Nijhuis, E.H.; Maat, M.J.; Zeegers, I.W.E.; Waalwijk, C.; Veen, Johannes A. van

doi:10.1016/0038-0717(93)90091-o

Cited by 40 publications

(21 citation statements)

References 28 publications

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“…Bcc is a known rhizosphere colonizer; populations of up to 10 5 CFU per g of root have been identified on the roots of peas (22). Other plant hosts known to support Bcc in the rhizosphere include maize (18,33), tomatoes (39), wheat and lupine (1), and perennial ryegrass (35). It thus was theorized that populations of Bcc would be enriched by the presence of a plant root, and that otherwise low-and possibly undetectable-populations of Bcc would be detected in rhizosphere samples, but this was not demonstrated by our results.…”

Section: Discussionmentioning

confidence: 99%

Culture-Based and Non-Growth-Dependent Detection of the Burkholderia cepacia Complex in Soil Environments

Miller¹,

LiPuma

Parke

2002

Appl Environ Microbiol

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Burkholderia cepacia complex (Bcc) bacteria reside in soil, plant rhizospheres, and water, but their prevalence and distribution in outdoor environments is not clear. We sampled a variety of soil and rhizosphere environments with which people may have contact: playgrounds, athletic fields, parks, hiking trails, residential yards, and gardens. A total of 91 sites was sampled in three large U.S. cities. In the first phase of the study, putative Bcc isolates were recovered on Burkholderia cepacia selective agar and trypan blue tetracycline medium and subsequently examined for biochemical reactivity and growth at 32 and 22°C. Isolates were further examined by PCR assays targeting Bcc-specific ribosomal DNA and recA gene sequences. Among the 1,013 bacterial isolates examined, 68 were identified as Bcc; 14 (15%) of 91 sampled sites yielded Bcc isolates. In the second phase, DNA was extracted directly from soil samples and examined with PCR assays targeting Bcc 16S rRNA gene sequences. Either 82 or 93% of the soil samples were positive for at least one Bcc genomovar, depending on the PCR assay system used. Cloning and sequencing were performed to check the specificity of the PCR assays. Sequence analysis of the 463-bp 16S rRNA inserts from eight clones indicated that all were from members of the Bcc. The four soil samples from which these clones were generated did not yield isolates identified as Bcc. Based on PCR detection, Bcc appears to be prevalent in soil from urban and suburban environments. Culture-based recovery of Bcc may underestimate environmental populations.

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Section: Discussionmentioning

confidence: 99%

Culture-Based and Non-Growth-Dependent Detection of the Burkholderia cepacia Complex in Soil Environments

Miller¹,

LiPuma

Parke

2002

Appl Environ Microbiol

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“…Bacterial stress caused by nutrient limitations may be a common situation occurring in the rhizosphere and it can be held responsible for the presence of a wide variety of physiologically different bacterial groups in this habitat. Isolation of bacteria from grass rhizosphere soil on low nutrient agar media (ten times diluted TSB and soil extract medium) resulted in the recovery of a large diversity of Gram-positive and Gram-negative species, belonging to the Proteobacteria, Actinobacteria and Firmicutes (Nijhuis et al 1993). Isolates were selected and tested for their capacity to colonize grass roots.…”

Section: Bacteriamentioning

confidence: 99%

The rhizosphere zoo: An overview of plant-associated communities of microorganisms, including phages, bacteria, archaea, and fungi, and of some of their structuring factors

Buée¹,

Boer

Martin³

et al. 2009

Plant Soil

396

236

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“…The selection and use of biocontrol strains therefore depend heavily on our knowledge of survival of the inoculant and its potential activity in the rhizosphere ecosystem of a particular plant and soil. Consequently, successful biological control with inoculated strains requires an understanding of the dynamics and composition of the bacterial communities colonizing the rhizosphere.Previous studies, employing cultivation-based, laboratory methods or microscopy, have shown that different bacterial populations are present or active at different stages of root development and that rhizosphere communities are distinct from those found in bulk soil (1,14,17,19,21,27,30). However, recent molecular studies involving PCR amplification of 16S rRNA genes (rDNA), question some of these results.…”

mentioning

confidence: 99%

“…Previous studies, employing cultivation-based, laboratory methods or microscopy, have shown that different bacterial populations are present or active at different stages of root development and that rhizosphere communities are distinct from those found in bulk soil (1,14,17,19,21,27,30). However, recent molecular studies involving PCR amplification of 16S rRNA genes (rDNA), question some of these results.…”

mentioning

confidence: 99%

Bacterial Origin and Community Composition in the Barley Phytosphere as a Function of Habitat and Presowing Conditions

Normander

Prosser

2000

Appl Environ Microbiol

141

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An understanding of the factors influencing colonization of the rhizosphere is essential for improved establishment of biocontrol agents. The aim of this study was to determine the origin and composition of bacterial communities in the developing barley (Hordeum vulgare) phytosphere, using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes amplified from extracted DNA. Discrete community compositions were identified in the endorhizosphere, rhizoplane, and rhizosphere soil of plants grown in an agricultural soil for up to 36 days. Cluster analysis revealed that DGGE profiles of the rhizoplane more closely resembled those in the soil than the profiles found in the root tissue or on the seed, suggesting that rhizoplane bacteria primarily originated from the surrounding soil. No change in bacterial community composition was observed in relation to plant age. Pregermination of the seeds for up to 6 days improved the survival of seed-associated bacteria on roots grown in soil, but only in the upper, nongrowing part of the rhizoplane. The potential occurrence of skewed PCR amplification was examined, and only minor cases of PCR bias for mixtures of two different DNA samples were observed, even when one of the samples contained plant DNA. The results demonstrate the application of culture-independent, molecular techniques in assessment of rhizosphere bacterial populations and the importance of the indigenous soil population in colonization of the rhizosphere.The use of antagonistic bacteria for the protection of crops against soilborne pathogens provides a promising environment-friendly alternative to chemical pesticides. However, the root colonization efficiency of introduced biocontrol strains is often limited, potentially reducing the effectiveness of protection (8,25,32). The selection and use of biocontrol strains therefore depend heavily on our knowledge of survival of the inoculant and its potential activity in the rhizosphere ecosystem of a particular plant and soil. Consequently, successful biological control with inoculated strains requires an understanding of the dynamics and composition of the bacterial communities colonizing the rhizosphere.Previous studies, employing cultivation-based, laboratory methods or microscopy, have shown that different bacterial populations are present or active at different stages of root development and that rhizosphere communities are distinct from those found in bulk soil (1,14,17,19,21,27,30). However, recent molecular studies involving PCR amplification of 16S rRNA genes (rDNA), question some of these results. Duineveld et al. (9) reported that the bacterial communities of the Chrysanthemum rhizosphere, as measured by denaturing gradient gel electrophoresis (DGGE) of 16S rDNA PCR products, changed very little with plant age and were similar to those of bulk soil. In contrast, different bacterial communities were identified in soil and in the root tissue of white clover and ryegrass by cluster analysis of a 16S rDNA clone library (16). Furthermore, DGG...

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Selection of bacteria suitable for introduction into the rhizosphere of grass

Cited by 40 publications

References 28 publications

Culture-Based and Non-Growth-Dependent Detection of the Burkholderia cepacia Complex in Soil Environments

Culture-Based and Non-Growth-Dependent Detection of the Burkholderia cepacia Complex in Soil Environments

The rhizosphere zoo: An overview of plant-associated communities of microorganisms, including phages, bacteria, archaea, and fungi, and of some of their structuring factors

Bacterial Origin and Community Composition in the Barley Phytosphere as a Function of Habitat and Presowing Conditions

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