Selection of Annonaceae Species for the Control ofSpodoptera frugiperda(Lepidoptera: Noctuidae) and Metabolic Profiling ofDuguetia lanceolataUsing Nuclear Magnetic Resonance Spectroscopy

Abstract

This study was performed to investigate the activity of 19 dichloromethane-soluble fractions obtained from the methanolic extracts of 10 Annonaceae species against the fall armyworm, Spodoptera frugiperda (J. E. Smith). The stem bark of Duguetia lanceolata A. St.-Hil. showed the highest insecticidal activity, with a median lethal time (LT50) of 61.4 h and a median lethal concentration (LC50) of 946.5 µg/ml of diet. The dichloromethane-soluble fractions from six D. lanceolata specimens were subjected to evaluation of their activities against S. frugiperda and metabolomic analysis using hydrogen (1H) nuclear magnetic resonance (NMR) spectroscopy. Although all of the samples affected S. frugiperda mortality, their insecticidal activities varied according to the sample used in the experiments. Using partial least squares regression of the results, the D. lanceolata specimens were grouped according to their metabolite profile and insecticidal activity. A detailed analysis via uni- and bidimensional NMR spectroscopy showed that the peaks in the 1H NMR spectra associated with increased insecticidal activity could be attributed to 2,4,5-trimethoxystyrene, which suggests that this substance is involved in the insecticidal activity of the stem bark fraction of D. lanceolata.

Advances in Control Strategies against Spodoptera frugiperda. A Review

“…This same amide (Figure 13) was also evaluated by ingestion, and at the lowest concentration (1 mg/kg), produced a mortality of 83.3% [43]. In another work, Alves et al [31] investigated the activity against first instar larvae of S. frugiperda of 19 dichloromethane soluble fractions obtained from the metabolic extracts of 10 species of the Annonaceae family. The crust of the stem of Duguetia lanceolata (A. St.-Hil).…”

“…The results indicate that the effect could be attributed to 2,4,5-trimethoxystyrene (Figure 14), suggesting that this compound may be implicated in the insecticide activity of the crust stem fraction of D. lanceolata [31]. In another work, Alves et al [31] investigated the activity against first instar larvae of S. frugiperda of 19 dichloromethane soluble fractions obtained from the metabolic extracts of 10 species of the Annonaceae family. The crust of the stem of Duguetia lanceolata (A. St.-Hil).…”

“…Therefore, many reports indicating the use of extracts of several types of plants against S. frugiperda consider these as effective, less expensive, and safer options for the environment and health. Some important metabolites contained in plant extracts and the effective doses found are described below [ 31 , 32 ].…”

“…In another work, Alves et al [ 31 ] investigated the activity against first instar larvae of S. frugiperda of 19 dichloromethane soluble fractions obtained from the metabolic extracts of 10 species of the Annonaceae family. The crust of the stem of Duguetia lanceolata (A. St.-Hil).…”

“…Then, another ten D. lanceolata specimens were analyzed by metabolomics and by uni and bidirectional RMN spectroscopy. The results indicate that the effect could be attributed to 2,4,5-trimethoxystyrene (Figure 14), suggesting that this compound may be implicated in the insecticide activity of the crust stem fraction of D. lanceolata [31]. Other authors have synthesized and evaluated the toxic effect of 11 amides on second instar larvae of S. frugiperda; two were of natural origin, isolated from Piper piressi (Yunck.…”