Selection of a Polyurethane Membrane for the Manufacture of Ventricles for a Totally Implantable Artificial Heart: Blood Compatibility and Biocompatibility Studies

Bélanger, Marie‐Claire; Marois, Yves; Roy, Raynald; Mehri, Yahye; Wagner, Éric; Zhang, Ze; King, Martin W.; Yang, Mingjing; Hahn, Charles; Guidoin, Robert

doi:10.1046/j.1525-1594.2000.06504.x

Cited by 60 publications

(43 citation statements)

References 36 publications

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“…The use of M3 membrane (PAES, sterilized by gamma rays) provoked a loss of EA.hy926 cell viability. Our results are similar to those of Bélanger et al (2000) [27]. Indeed, they found that the Carbothane, Chronoflex and Tecoflex membranes sterilized by ethylene oxide maintained the highest percentage of monocyte viability after 72 h of incubation ([70%), while none of the gamma rays-sterilized membranes displayed any cell viability.…”

Section: Discussionsupporting

confidence: 92%

Cytotoxic effects exerted by polyarylsulfone dialyser membranes depend on different sterilization processes

et al. 2009

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Polyarylsulfone group is one of the most important polymeric materials used in the biomedical field, due to its excellent properties, such as good thermal, chemical, and mechanical stability. There are three important polyarylsulfone polymers, all of which have excellent electrical properties: polysulfone (PSu), polyarylsulfone (PAS) and polyarylethersulfone (PAES). All these polymers have excellent creep, radiation and high temperature resistance. In this study, we aimed to determine the effect of three sterilization processes (steam, ethylene oxide and gamma rays) on cytotoxicity of polyarylsulfone dialysis membranes. Ten long-term dialysis patients and ten age-matched healthy controls were enrolled in our study. We analysed (1) serum effect on cultured endothelial cell viability using MTT assay and (2) lipid peroxidation assessed by serum malondialdehyde (MDA) formation at the beginning (T0), the middle (T2) and the end (T4) of haemodialysis (HD) session. Our results clearly showed that steam-sterilized membranes improve endothelial cell viability when compared to ethylene oxide or gamma rays-sterilized ones. Moreover, there is a increased generation of MDA in patients sera during HD session. The serum MDA concentration was about 3, 6 and 10 times higher, respectively, for steam, ethylene oxide and gamma rays sterilization procedures when compared to the MDA amount in healthy subject sera. We concluded that using steam instead of ethylene oxide or gamma rays for sterilization may improve the biocompatibility of polyarylsulfone membranes.

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Section: Discussionsupporting

confidence: 92%

Cytotoxic effects exerted by polyarylsulfone dialyser membranes depend on different sterilization processes

et al. 2009

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“…The supernatant from each tube was transferred to a well in a 96-well plate where the absorbance was measured at 545 nm. The percent hemolysis was calculated as follows 11 :…”

Section: Hemolysismentioning

confidence: 99%

Hemocompatibility evaluation of poly(diol citrate) in vitro for vascular tissue engineering

Motlagh

Allen

Hoshi

et al. 2007

J Biomedical Materials Res

127

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One of the ongoing challenges in tissue engineering is the synthesis of a hemocompatible vascular graft. Specifically, the material used in the construct should have antithrombogenic properties and support the growth of vascular cells. Our laboratory has designed a novel biodegradable, elastomeric copolymer, poly(1,8-octanediol citrate) (POC), with mechanical and degradation properties suitable for vascular tissue engineering. The hemocompatibility of POC in vitro and its ability to support the attachment and differentiation of human aortic endothelial cell (HAEC) was assessed. The thrombogenicity and inflammatory potential of POC were assessed relative to poly(l-lactide-co-glycolide) and expanded poly(tetrafluoroethylene), as they have been used in FDA-approved devices for blood contact. Specifically, platelet aggregation and activation, protein adsorption, plasma clotting, and hemolysis were investigated. To assess the inflammatory potential of POC, the release of IL-1beta and TNF-alpha from THP-1 cells was measured. The cell compatibility of POC was assessed by confirming HAEC differentiation and attachment under flow conditions. POC exhibited decreased platelet adhesion and clotting relative to control materials. Hemolysis was negligible and protein adsorption was comparable to reference materials. IL-1beta and TNF-alpha release from THP-1 cells was comparable among all materials tested, suggesting minimal inflammatory potential. POC supported HAEC differentiation and attachment without any premodification of the surface. The results described herein are encouraging and suggest that POC is hemocompatible and an adequate candidate biomaterial for in vivo vascular tissue engineering.

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“…Polyurethanes are also recognized with their biocompatibility and biodegradation character [10] and hence have an important potential in wound dressing applications [11][12][13]. In literature it is emphasized that the chemistry of a polyurethane membrane has an important effect on biocompatibility [14]. In our previous study, triglyceride oil-modified polyurethane films for wound dressing applications were prepared from linseed oil-based hydroxyl containing material and hexamethylene diisocyanate (HMDI) and/or diphenylmethylene diisocyanate (MDI) [15].…”

Section: Introductionmentioning

confidence: 99%

Fatty acid-based polyurethane films for wound dressing applications

Gultekin

Atalay-Oral

Erkal

et al. 2008

J Mater Sci: Mater Med

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Fatty acid-based polyurethane films were prepared for use as potential wound dressing material. The polymerization reaction was carried out with or without catalyst. Polymer films were prepared by casting-evaporation technique with or without crosslink-catalyst. The film prepared from uncatalyzed reaction product with crosslink-catalyst gave slightly higher crosslink density. The mechanical tests showed that, the increase in the tensile strength and decrease in the elongation at break is due to the increase in the degree of crosslinking. All films were flexible, and resisted to acid solution. The films prepared without crosslink-catalyst were more hydrophilic, absorbed more water. The highest permeability values were generally obtained for the films prepared without crosslink catalyst. Both the direct contact method and the MMT test were applied for determination of cytotoxicity of polymer films and the polyurethane film prepared from uncatalyzed reaction product without crosslink-catalyst showed better biocompatibility property, closest to the commercial product, Opsite Ò .

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Selection of a Polyurethane Membrane for the Manufacture of Ventricles for a Totally Implantable Artificial Heart: Blood Compatibility and Biocompatibility Studies

Cited by 60 publications

References 36 publications

Cytotoxic effects exerted by polyarylsulfone dialyser membranes depend on different sterilization processes

Cytotoxic effects exerted by polyarylsulfone dialyser membranes depend on different sterilization processes

Hemocompatibility evaluation of poly(diol citrate) in vitro for vascular tissue engineering

Fatty acid-based polyurethane films for wound dressing applications

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