Induction of jlbA mRNA synthesis for a putative bZIP protein of Aspergillus nidulans by amino acid starvation 2.1 Abstract 2.2 Introduction 2.3 Experimental procedures 2.3.1 Strains 2.3.2 Media and growth conditions 2.3.3 Recombinat DNA techniques 2.4 Results 2.4.1 Identifaction of a new jun-like bZIP encoding gene in A. nidulans 2.4.2 The jlbA gene encodes a transcript with two putative intervening sequences 2.4.3 The JLBA protein contains a DNA binding region that shares similarities with other proteins with a bZIP-DNA-binding motif 2.4.4 jlbA is a single copy gene and localized on chromosome VII 2.4.4 The jlbA transcription level is strongly induced by addition of the amino acid analogue 3-amino-1,2,4 triazole (3AT) 2.5 Discussion 2.6 References II Table of Content -Chapter III -Functional analysis of the ribosomal RPS26 isogenes in the yeast Saccharomyces cerevisiae 3.1 Abstract 3.2 III Table of Content -Chapter IV -Evolution of DAHP synthase encoding genes in the yeast Saccharomyces cerevisiae 4.1 Abstract 4.2 Introduction 4.3 Experimental procedures 4.3.1 Media materials and supplements 4.3.2 Strains and Plasmids 4.3.3 AroH mutations 4.3.4 Enzyme assays 4.4 Results 4.4.1 A tryptophan-inhibitable DAHP synthase regenerates growth of a gcn4-103 mutant yeast strain in the presence of the two aromatic amino acids, phenylalanine and tyrosine 4.4.2 Expression of a heterologous DAHP synthase is significantly higher when driven by the yeast ARO3 promoter when compared to the yeast ARO4 promoter 4.4.3 A single Ala177Lys substitution changes the tryptophaninhibitable DAHP synthase towards a trytophan-and tyrosine-inhibitable enzyme 4.4.4 The yeast Aro4p DAHP synthase is similarly regulated by tryptophan and tyrosine as an Ala177Lys AroHp mutant enzyme 4.4.5 A single Pro165Gly substitution results in an Aro4p yeast DAHP synthase, which is efficiently inhibitable by tyrosine and tryptophan 4.5 Discussion 4.6 Acknowledgements 4.7 References Curriculum vitae IV Abbreviations Abbreviations 3AT 3-amino-1,2,4-triazole 5MT 5-methyl-tryptophan AA anthranilic acid AAS anthranilate synthase ADIC 2-amino-2-deoxyisochorismate ADP adenosine-5'-diphosphate ATP adenosine-5'-triphosphate bp base pair BSA bovine serum albumin CA chorismic acid CDRP carboxy-phenylamino-1-deoxyribulose-5-phosphate CM chorismate mutase CPRE 'cross-pathway' control recognition element Summary -Chapter I -Hinnebusch & Natarajan, 2002). In A. nidulans, A. niger, and Neuropora crassa the same transcriptional regulation system is termed 'cross pathway' control and acts via Gcn4p related proteins (Paluh . This transcriptional regulation is genetically balanced in such a fine way that starvation for one single amino acid or imbalances in the amino acid pool are capable to induce the system control, while growth on minimal vitamin medium lacking any amino acid supplementation and the subsequent expression of all 20 amino acids does not result in an induction effect.the regulation of the transcription within the yeast S. cerevisiae (Zhang et al., 2000).Assembly of...
