Selection, Identification and Optimization of the Growth Water Probiotic Consortium of Mangrove Ecosystems as Bioremediation and Biocontrol in Shrimp Ponds

Abstract

Shrimp aquaculture is an activity that potentially generates organic waste. The accumulation of organic matter is becoming one of the main factors causing the emergence of disease. Problem-solving approach that is most effective is through bioremediation. The aims of this study were to select, identify and cultivate bacteria from mangrove sediments from Cilacap, Rembang and Banyuwangi which potentially as probiotic consortium of bioremediation activity and biocontrol. The results showed that total of 45 isolates (proteolytic), 35 isolates (amylolytic), 35 isolates (lipolytic), and 18 isolates (cellulolytic). There were 59 bacterial isolates had antibacterial activity of vibrio (V. harveyi, V. alginolyticus, V. vulnificus and V. anguilarum). Based on the identification of 16 S-rRNA genes, 4 isolates showed that the C2 isolate was identified as Bacillus subtilis, C11 isolate was identified as Bacillus firmus, C13 and C14 isolates were identified as B. Flexus. This study concluded that cultivation of Bacillus subtilis C2 optimum at 2% molase and yeast extract 0.5% at pH 8 and 30 0C. Bacillus firmus C11 optimum at 2% molase and yeast extract 0.5% at pH 8 and 30 0C. Bacillus flexus C13 optimum at 2% glucose and yeast extract 0.5% at pH 8 and 30 0C. Bacillus flexus C14 optimum at 4% molase and yeast extract 0.25% at pH 8 and 30 0C. The result of culture applications of 4 isolates showed an effect of increasing shrimp weight by 141, 9% compared by the control.

Isolation and molecular identification of proteolytic bacteria from vaname shrimp (Lithopenaeus Vannamei) ponds as probiotic agents

“…Therefore, the maximum protease production (141.46 U/mg) with a C-N source and 1 M NaCl was obtained for 24 h, at a pH 8 below 250 rpm. This indicated that the isolation of bacteria from mangrove sediments increased the weight of vannamei shrimp by 141.9%, showing that the isolates had good extracellular properties in degrading protein (53).…”

SALURAN PENCERNAAN UDANG JERBUNG, Penaeus merguiensis

“…Kemudian kurva ketiga isolat tersebut menunjukkan penurunan yang berarti fase kematian. Pertumbuhan bakteri dapat dipengaruhi oleh beberapa faktor di antaranya yaitu pH, nutrisi, dan suhu (Setyati et al, 2015). Menurut Rolfe et al (2012), kurva pertumbuhan menunjukkan adanya aktivitas pertumbuhan ataupun pembelahan sel suatu mikroorganisme yang diawali dengan fase pertumbuhan dan diakhiri dengan fase kematian.…”

Optimasi pH Dan Salinitas Media Kultur Terhadap Pertumbuhan Dan Aktivitas Protease Ektraseluler Bakteri Bacillus Firmus Dari Ekosistem Padang Lamun Nusa Lembongan – Bali.

“…Supernatan di buang dan natan yang didapatkan dilarutkan dengan larutan PBS sebanyak 10 ml lalu di divortex. Selanjutnya diamati nilai OD-nya dengan spektrofotometer pada panjang gelombang 600 nm (Setyati et al, 2014). Data hasil pengukuran OD pada A600 kemudian di konversi dengan persamaan standart Mc Farland menjadi satuan sel/ml.…”