Selection for lean growth and food intake leads to correlated changes in innate immune traits in Large White pigs

Clapperton, M.; Bishop, Stephen; Glass, Elizabeth J.

doi:10.1017/asc200691

Cited by 14 publications

(13 citation statements)

References 54 publications

(70 reference statements)

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“…In order to find markers that reflect resistance towards a wide range of infectious diseases, we chose the immune traits used in this study because they are repeatable, easy to measure compared to other labour-intensive assays for cell function and relatively stable across time (Clapperton et al, 2005a). Also, since levels of these traits were shown to phenotypically negatively correlate with performance (Clapperton et al, 2005a) and to be influenced by pig genotype (Clapperton et al, 2006), this suggests that these markers may both be heritable and genetically correlate with animal health status. The focus on traits that are largely representative of the innate immune system was also deliberate, as this is more likely to confer appropriate responses to a wide variety of diseases than measures of acquired or adaptive immunity.…”

Section: Discussionmentioning

confidence: 99%

“…Pigs from sources 1 and 4 were subdivided into lines, and line effects from source 4 (i.e. Roslin Institute farm E) were previously presented by Clapperton et al (2006). Many of the traits were right skewed and required log-transformation prior to analysis.…”

Section: Methodsmentioning

confidence: 99%

“…This is because our past work has demonstrated that amongst apparently healthy pigs from a farm of intermediate health status, the proportions of specific PBML subsets, B cells, monocytes and CD11R1 1 cells were negatively correlated with performance (Clapperton et al, 2005a). Also from the same pigs, we compared two divergent lines selected for lean growth under restricted feeding (LGR) and observed that high LGR line pigs had higher numbers of total white blood cells (WBC), CD8a 1 cells and CD11R1 1 cells than low LGR line pigs (Clapperton et al, 2006). Overall, these findings would suggest that specific PBML subsets correlate with performance, and can be influenced by genotype.…”

Section: Introductionmentioning

confidence: 99%

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Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance

Clapperton

Glass

Bishop

2008

Animal

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Indicator traits used to select pigs for increased resistance to infection or improved health must be heritable and, preferably, be associated with improved performance. We estimated the heritability of a range of immune traits and their genetic and phenotypic correlations with growth performance. We measured immune traits on 589 pigs and performance on 1941 pigs from six farms, three of which were classified as 'high health status' (i.e. specific pathogen-free) and three were of lower health status. All pigs were apparently healthy. Immune traits were total white blood cells (WBC), and peripheral blood mononuclear leucocyte (PBML) subsets positive for CD4, CD8a, gamma delta (gd) T cell receptor, CD11R1 (natural killer cell marker), B cell and monocyte markers at the start and the end of standard growth performance tests. At both time points, all immune traits were moderately to highly heritable except for CD8a 1 cells. At end of test, heritability estimates (h 2 ) (6s.e.) were 0.18 (60.11) for total WBC count. For PBML subset proportions, the heritabilities were 0.52 (60.14) for gd TCR 1 cells, 0.62 (60.14) for CD4 1 cells, 0.44 (60.14) for CD11R1 1 cells, 0.58 (60.14) for B cells and 0.59 (60.14) for monocytes. Farm health status affected the heritabilities for WBC, being substantially higher on lower health status farms, but did not have consistent effects on heritabilities for the PBML subsets. There were significant negative genetic correlations between numbers and proportions of various PBML subsets and performance, at both start and end of test. In particular, the proportion of PBML cells that were CD11R1 1 cells, at end of test, was strongly correlated with daily gain (r g 5 20.72; P , 0.01). There were also weaker but significant negative phenotypic correlations between PBML subsets measured at end of test and performance, for gd 1 T cells, CD8a 1 , CD11R1 1 cells, B cells or monocytes. Phenotypic correlations with daily gain were generally lower at the start of test than at the end of test. These results show that most of the major pig PBML subsets are heritable, and that systemic levels of several of these PBML subsets are genetically negatively correlated with performance. This approach provides a basis for using immune trait markers when selecting boars that can produce higher-performing progeny.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance

Clapperton

Glass

Bishop

2008

Animal

Self Cite

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“…Porcine selection for high immune response was found associated with enhanced weight gain 8,9, whereas selection for lean growth was observed associated with higher numbers of several leukocyte subtypes 10. In addition, Galina-Pantoja et al 29 provide evidence for the association of several leukocyte subsets with growth traits.…”

Section: Discussionmentioning

confidence: 99%

“…Conversely, other studies report that selection for high immune response associates with enhanced weight gain 8,9. Clapperton and colleagues 10 show that selection for lean growth associates with higher numbers of several types of lymphocytes and monocytes in Large White pigs.…”

Section: Introductionmentioning

confidence: 99%

PBMC Transcription Profiles of Pigs with Divergent Humoral Immune Responses and Lean Growth Performance

Adler¹,

Muráni²,

Ponsuksili³

et al. 2013

Int. J. Biol. Sci.

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Background: The identification of key genes and regulatory networks in the transcriptomic responses of blood cells to antigen stimulation could facilitate the understanding of host defence and disease resistance. Moreover, genetic relationships between immunocompetence and the expression of other phenotypes, such as those of metabolic interest, are debated but incompletely understood in farm animals. Both positive and negative associations between immune responsiveness and performance traits such as weight gain or lean growth have been reported.We designed an in vivo microarray study of transcriptional changes in porcine peripheral blood mononuclear cells (PBMCs) during the immune response to tetanus toxoid (TT) as a model antigen for combined cellular (Th1) and humoral (Th2) responses. The aim of the study was to investigate the responsiveness of PBMCs against the background of divergent lean growth (LG) performance and anti-TT antibody (AB) titers and to compare lean growth and humoral immune performance phenotypes.Results: In general, high LG phenotypes had increased cellular immune response transcripts, while low AB phenotypes had increased transcripts for canonical pathways that represented processes of intracellular and second messenger signaling and immune responses. Comparison of lean growth phenotypes in the context of high AB titers revealed higher cellular immune response transcripts in high LG phenotypes. Similar comparisons in the context of low AB titers failed to identify any corresponding pathways. When high and low AB titer phenotypes were differentially compared, low AB phenotypes had higher cellular immune response transcripts on a low LG background and higher cell signaling, growth, and proliferation transcripts on a high LG background.Conclusions: Divergent phenotypes of both lean growth performance and humoral immune response are affected by significant and functional transcript abundance changes throughout the immune response. The selected high-performance phenotypes demonstrated both high AB titers and increased transcript abundance of cellular immune response genes, which were possibly offset by lower expression of other cellular functions. Further, indications of compensatory effects were observed between cellular and humoral immune responses that became visible only in low-performance phenotypes.

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Comparative transcriptomic analysis by RNA-seq to discern differential expression of genes in liver and muscle tissues of adult Berkshire and Jeju Native Pig

Sodhi

Song

Ghosh

et al. 2014

Gene

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Selection for lean growth and food intake leads to correlated changes in innate immune traits in Large White pigs

Cited by 14 publications

References 54 publications

Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance

Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance

PBMC Transcription Profiles of Pigs with Divergent Humoral Immune Responses and Lean Growth Performance

Comparative transcriptomic analysis by RNA-seq to discern differential expression of genes in liver and muscle tissues of adult Berkshire and Jeju Native Pig

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