Selection drives convergent gene expression changes during transitions to co-sexuality in haploid sexual systems

Cossard, Guillaume; Nehr, Zofia; Cruaud, Corinne; Cock, J. Mark; Lipinska, Agnieszka P.; Coelho, Susana M.

doi:10.1038/s41559-022-01692-4

Cited by 18 publications

(29 citation statements)

References 73 publications

(90 reference statements)

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“…To our knowledge, this analysis is the first comparative analysis of SBGE between gynodioecious species and dioecious species from independent transitions to dioecy. Indeed, despite numerous analyses of SBGE conducted to date, very few have been done in a comparative way (but see ( 65, 66, 69, 73 )). This limits our understanding of its evolution and the evolutionary forces that shape it, especially in a transition from hermaphroditism to dioecy or gonochorism.…”

Section: Discussionmentioning

confidence: 99%

Evolution of sex-biased gene expression during transitions to separate sexes in theSilenegenus

Prentout,

Muyle,

Zemp

et al. 2023

Preprint

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Sexual dimorphism is widespread among species with separate sexes and its extent is thought to be governed by the differential expression of thousands of genes between males and females (known as Sex-Biased Genes, hereafter SBGs). SBGs have been studied in numerous species, but rarely in a comparative way, which curtails our understanding of their evolution, especially during multiple independent transitions to separate sexes. We sequenced the transcriptomes of nine dioecious species, two gynodioecious species (separate females and hermaphrodites) and two hermaphrodite species from theSilenegenus. Our dataset provides access to three independent transitions to dioecy (dating from less than 1 Myo to about 11 Myo). We demonstrated that male-biased expression emerges first during a transition to separate sexes, later followed by female-biased genes. Furthermore, we showed that, despite a mixture of selective regimes, positive selection significantly affects the evolution of some SBGs. Overall, this study provides new insights on the causes of SBG evolution during transitions to separate sexes.TeaserThis study describes the evolution of sex-biased gene expression during a transition to separate sexes in plants.

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Section: Discussionmentioning

confidence: 99%

Evolution of sex-biased gene expression during transitions to separate sexes in theSilenegenus

Prentout,

Muyle,

Zemp

et al. 2023

Preprint

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“…RNA-seq analysis was performed to compare the abundances of gene transcripts in the mutants bas-1 and bas-2 and wild-type sporophytes. RNA-seq datasets were generated from triplicate samples of each genotype, and individuals were grown synchronously as described previously in standard culture conditions ( Coelho et al, 2012b ; Cossard et al, 2022 ). Germlings were filtered through a nylon mesh to recover only thalli at the two- to five-cell stage.…”

Section: Methodsmentioning

confidence: 99%

“…A gene was considered to be expressed if the TPMmean was above the 5th percentile (as in Cossard et al, 2022 ; Lipinska et al, 2019 ). About 4% of the genes in each sample had TPMmean values under this threshold and were considered not to be expressed in our samples ( Table S9 ).…”

Section: Methodsmentioning

confidence: 99%

The baseless mutant links protein phosphatase 2A with basal cell identity in the brown alga Ectocarpus

et al. 2023

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The first mitotic division of the initial cell is a key event in all multicellular organisms and is associated with the establishment of major developmental axes and cell fates. The brown alga Ectocarpus has a haploid-diploid life cycle that involves the development of two multicellular generations: the sporophyte and the gametophyte. Each generation deploys a distinct developmental programme autonomously from an initial cell, the first cell division of which sets up the future body pattern. Here, we show that mutations in the BASELESS (BAS) gene result in multiple cellular defects during the first cell division and subsequent failure to produce basal structures during both generations. BAS encodes a type B″ regulatory subunit of protein phosphatase 2A (PP2A), and transcriptomic analysis identified potential effector genes that may be involved in determining basal cell fate. The bas mutant phenotype is very similar to that observed in distag (dis) mutants, which lack a functional Tubulin-binding co-factor Cd1 (TBCCd1) protein, indicating that TBCCd1 and PP2A are two essential components of the cellular machinery that regulates the first cell division and mediates basal cell fate determination.

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“…A gene was considered to be expressed if the TPMmean was above the 5 th percentile (as in Cossard et al, 2022; Lipinska et al, 2019). About 4% of the genes in each sample had TPMmean values under this threshold and were considered not to be expressed in our samples (Table S9).…”

Section: Methodsmentioning

confidence: 99%

“…Comparative transcriptome analyses RNA-seq analysis was performed to compare the abundances of gene transcripts in the mutants bas-1 and bas-2 and wild-type sporophytes. RNA-seq datasets were generated from triplicate samples of each genotype, and individuals were grown synchronously as described previously in standard culture conditions (Coelho et al, 2012b;Cossard et al, 2022). Germlings were filtered through a nylon mesh to recover only thalli at the 2-5 cell stage.…”

Section: Bas and Dis Gene Expression Estimation During The Ectocarpus...mentioning

confidence: 99%

The baseless mutant links protein phosphatase 2A with basal cell identity in the brown alga Ectocarpus

Zheng

Yao

Henschen

et al. 2022

Preprint

Self Cite

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The first mitotic division of the initial cell is a key event in all multicellular organisms and is usually concomitant with the establishment of major developmental axes and cell fates. The brown alga Ectocarpus has a haploid-diploid life cycle that involves the development of two multicellular and independent generations, the sporophyte and the gametophyte. Each generation deploys a distinct developmental program autonomously from an initial cell, whose first cell division sets up the future body pattern. Here, we show that mutations in the BASELESS (BAS) gene result in multiple cellular defects during the first division of the initial cell and subsequently failure to produce basal structures (rhizoids and prostrate filaments) during both generations of the life cycle. Cloning-by-sequencing revealed that BAS encodes a type B" regulatory subunit of protein phosphatase 2A, and transcriptomic analysis of early developmental stages uncovered potential effector genes involved in basal cell fate determination in this organism. The bas mutant phenotype is very similar to that observed in the distag (dis) mutants, which lack a functional TBCCd1 protein, at both the cellular and morphological levels. The high level of similarity of the dis and bas mutant phenotypes indicate that TBCCd1 and PP2A are two critical components of the cellular machinery that regulates the division of the initial cell and mediates the establishment of basal cell fate in the developing thallus.

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Selection drives convergent gene expression changes during transitions to co-sexuality in haploid sexual systems

Cited by 18 publications

References 73 publications

Evolution of sex-biased gene expression during transitions to separate sexes in theSilenegenus

Evolution of sex-biased gene expression during transitions to separate sexes in theSilenegenus

The baseless mutant links protein phosphatase 2A with basal cell identity in the brown alga Ectocarpus

The baseless mutant links protein phosphatase 2A with basal cell identity in the brown alga Ectocarpus

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