Selection based on CD133 and high aldehyde dehydrogenase activity isolates long-term reconstituting human hematopoietic stem cells

Hess, David A.; Wirthlin, Louisa; Craft, Timothy P.; Herrbrich, Phillip E.; Hohm, Sarah A.; Lahey, Ryan; Eades, William; Creer, Michael H.; Nolta, Jan A.

doi:10.1182/blood-2005-06-2284

Cited by 248 publications

(193 citation statements)

References 45 publications

Supporting

Mentioning

181

Contrasting

Unclassified

Order By: Relevance

“…Published studies have shown that the ALDH1 bright / CD133 positive cells contain both short and long term repopulating human HSCs and in contrast to ALDH1 bright /CD133 negative , cells have faster engraftment and repopulation capacity (7). Our data confirms published studies showing that most ALDH1 bright /SSC low cells display phenotypic markers of primitive hematopoietic cells, that is, CD34, CD117, and CD133 (7,11,13).…”

Section: Correlation Of Marker Expression In Cells From Hpc-a Samplessupporting

confidence: 81%

“…ALDH1 dim cells from human cord blood can initiate short-term progenitors in mice xenograft models, whereas the ALDH1 bright cells can initiate long-term cultures and grow as xenografts (10). Hess et al (7) reported that in human cord blood, ALDH1 bright / CD34 negative cells might represent a primitive progenitor population, whereas Mirabelli et al (13) reported that in human bone marrow ALDH1 bright /CD34 negative , Figure 3A shows that 0.13% of the cells had CD34 bright and CD90 mid expression (gate R3) while in contrast 0.11% of the cells were CD34 bright with CD90 negative expression (gate R5). Dot plot 3B shows that 0.25% of the cells had CD34 bright and CD117 dim-bright expression while 0.30% of the cells had CD34 dim and CD117 dim expression (gate R4) or 0.12% of the cells had CD34 negative and CD117 dim expression (gate R5).…”

Section: Figmentioning

confidence: 88%

“…Hess et al (7) purified cells with high ALDH1 activity from human cord blood and showed that they coexpressed stem cell markers such as, CD34 and CD133. Pearce and Bonnet (8) purified ALDH1 positive, lineage negative cells from murine bone marrow and showed that they overlapped with the side population (SP) cells.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Electronic volume, aldehyde dehydrogenase, and stem cell marker expression in cells from human peripheral blood apheresis samples

Sharma

Shariatmadar

Krishan

2010

Cytometry Part B Clinical

View full text Add to dashboard Cite

Section: Correlation Of Marker Expression In Cells From Hpc-a Samplessupporting

confidence: 81%

Section: Figmentioning

confidence: 88%

See 1 more Smart Citation

Electronic volume, aldehyde dehydrogenase, and stem cell marker expression in cells from human peripheral blood apheresis samples

Sharma

Shariatmadar

Krishan

2010

Cytometry Part B Clinical

View full text Add to dashboard Cite

“…Thus, ALDH1A1-expressing cells have a crucial role in the initiation and progression of esophageal squamous cell carcinoma. ALDH1A1 is a predominant isoform of aldehyde dehydrogenase family in mammals located in the cytoplasm 15,16 and is originally considered as a marker for normal hematopoietic progenitor cells, 17 and is also found in cancer cells with stem cell property, that is, cancer stem-like cells, in several malignant tumors including leukemia, 9 breast cancer 7 and lung cancer. 8,18 Wang et al 19 reported that ALDH1A1 was located in the nuclei of esophageal squamous cell carcinoma cells.…”

Section: Discussionmentioning

confidence: 99%

ALDH1A1 defines invasive cancer stem-like cells and predicts poor prognosis in patients with esophageal squamous cell carcinoma

et al. 2014

View full text Add to dashboard Cite

Invasion and metastasis are the major cause of deaths in patients with esophageal cancer. In this study, we isolated cancer stem-like cells from an esophageal squamous cell carcinoma cell line EC109 based on aldehyde dehydrogenase 1A1 (ALDH1A1), and found that ALDH1A1 high cells possessed the capacities of self-renewal, differentiation and tumor initiation, indications of stem cell properties. To support their stemness, ALDH1A1 high cells exhibited increased potential of invasion and metastasis as compared with ALDH1A1 low cells. ALDH1A1 high esophageal squamous cell carcinoma cells expressed increased levels of mRNA for vimentin, matrix metalloproteinase 2, 7 and 9 (MMP2, MMP7 and MMP9), but decreased the level of E-cadherin mRNA, suggesting that epithelial-mesenchymal transition and secretary MMPs may be attributed to the high invasive and metastatic capabilities of ALDH1A1 high cells. Furthermore, we examined esophageal squamous cell carcinoma specimens from 165 patients and found that ALDH1A1 high cells were associated with esophageal squamous dysplasia and the grades, differentiation and invasion depth, lymph node metastasis and UICC stage of esophageal squamous cell carcinoma, as well as poor prognosis of patients. Our results provide the strong evidence that ALDH1A1 high cancer stem-like cells contribute to the invasion, metastasis and poor outcome of human esophageal squamous cell carcinoma. Modern Pathology (2014) 27, 775-783; doi:10.1038/modpathol.2013.189; published online 8 November 2013Keywords: aldehyde dehydrogenase 1A1; cancer stem-like cells; esophageal squamous carcinoma; invasion; metastasis; prognosis Esophageal squamous cell carcinoma is one of the most frequent fatal malignancies in the area from northern Iran to north-central China ('Asian esophageal cancer belt'). 1,2 The 5-year survival rate of esophageal squamous cell carcinoma patients after surgery and chemotherapy remains low owing to highly invasive and metastatic nature of esophageal squamous cell carcinoma. Cancer stem-like cells are a small subpopulation within tumors with the capacities for self-renewal and generating heterogeneous tumor cell lineages. 3 Recent studies suggest that cancer stem-like cells are responsible for invasion and metastasis of many tumor types. We have reported that cancer stem-like cells possess higher capability of invasion and metastasis in solid tumors. [4][5][6] However, the biomarkers related to invasion and metastasis of cancer stem-like cells

show abstract

“…HSCs with long-term repopulating ability have been identified in bone marrow and cord blood, based on the combined expression of CD34 and ALDH [51,52]. High ALDH expression has also been combined with CD133 to select for human HSCs from bone marrow [78]. Sca-1 (stem cell antigen 1, Ly-6A/E) is an 18 kDa phosphatidylinositol-anchored protein expressed on murine multipotent HSCs, and is frequently used in combination with negative-selection for a number of cell surface markers characteristic of commitment toward haematolymphoid lineages (Lin − ).…”

Section: Bone Marrowmentioning

confidence: 99%

Attributes of adult stem cells

Alison

Islam

2008

The Journal of Pathology

153

115

View full text Add to dashboard Cite

While cultured embryonic stem (ES) cells can be harvested in abundance and appear to be the most versatile of cells for regenerative medicine, adult stem cells also hold promise, but the identity and subsequent isolation of these comparatively rare cells remains problematic in most tissues, perhaps with the notable exception of the bone marrow. The ability to continuously self-renew and produce the differentiated progeny of the tissue of their location are their defining properties. Identifying surface molecules (markers) that would aid in stem cell isolation is a major goal. Considerable overlap exists between different putative organspecific stem cells in their repertoire of gene expression, often related to self-renewal, cell survival and cell adhesion. More robust tests of 'stemness' are now being employed, using lineage-specific genetic marking and tracking to show production of long-lived clones and multipotentiality in vivo. Moreover, the characterization of normal stem cells in specific tissues may provide a dividend for the treatment of cancer. The successful treatment of neoplastic disease may well require the specific targeting of neoplastic stem cells, cells that may well have many of the characteristics of their normal counterparts.

show abstract

Selection based on CD133 and high aldehyde dehydrogenase activity isolates long-term reconstituting human hematopoietic stem cells

Cited by 248 publications

References 45 publications

Electronic volume, aldehyde dehydrogenase, and stem cell marker expression in cells from human peripheral blood apheresis samples

Electronic volume, aldehyde dehydrogenase, and stem cell marker expression in cells from human peripheral blood apheresis samples

ALDH1A1 defines invasive cancer stem-like cells and predicts poor prognosis in patients with esophageal squamous cell carcinoma

Attributes of adult stem cells

Contact Info

Product

Resources

About