Selection and verification of the combination of reference genes for RT-qPCR analysis in rat adrenal gland development

Xia, Xuan; Liu, Yi; Lv, Liang; Chen, Yawen; Wang, Hui

doi:10.1016/j.jsbmb.2021.105821

Cited by 6 publications

(2 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MiR-16-5p was found to show the most stable expression levels across the samples with a p -value of 0.452 in comparison to RNU6B which had a p -value of 0.001. In the case of RNA reference genes, PPIA [ 53 ] and GAPDH [ 54 ] were compared. Here, PPIA was found to show the most stable expression levels across the samples with a p -value of 0.019 in comparison to GAPDH which had a p -value of 0.003.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Adrenal miRNA-Based Dysregulations in Cushing’s Syndrome

Vetrivel

Zhang

Engel

et al. 2022

IJMS

View full text Add to dashboard Cite

MiRNAs are important epigenetic players with tissue- and disease-specific effects. In this study, our aim was to investigate the putative differential expression of miRNAs in adrenal tissues from different forms of Cushing’s syndrome (CS). For this, miRNA-based next-generation sequencing was performed in adrenal tissues taken from patients with ACTH-independent cortisol-producing adrenocortical adenomas (CPA), from patients with ACTH-dependent pituitary Cushing’s disease (CD) after bilateral adrenalectomy, and from control subjects. A confirmatory QPCR was also performed in adrenals from patients with other CS subtypes, such as primary bilateral macronodular hyperplasia and ectopic CS. Sequencing revealed significant differences in the miRNA profiles of CD and CPA. QPCR revealed the upregulated expression of miR-1247-5p in CPA and PBMAH (log2 fold change > 2.5, p < 0.05). MiR-379-5p was found to be upregulated in PBMAH and CD (log2 fold change > 1.8, p < 0.05). Analyses of miR-1247-5p and miR-379-5p expression in the adrenals of mice which had been exposed to short-term ACTH stimulation showed no influence on the adrenal miRNA expression profiles. For miRNA-specific target prediction, RNA-seq data from the adrenals of CPA, PBMAH, and control samples were analyzed with different bioinformatic platforms. The analyses revealed that both miR-1247-5p and miR-379-5p target specific genes in the WNT signaling pathway. In conclusion, this study identified distinct adrenal miRNAs as being associated with CS subtypes.

show abstract

Section: Methodsmentioning

confidence: 99%

Characterization of Adrenal miRNA-Based Dysregulations in Cushing’s Syndrome

Vetrivel

Zhang

Engel

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…The specific experimental conditions and data processing were referred to a previous study. 39 As a quantitative control, each sample was repeatedly detected 3 times. Specific primer sequences (Table S1 †) were synthesized by Wuhan Tianyi Huiyuan Biotechnology Co., Ltd.…”

Section: Papermentioning

confidence: 99%