Selection and Validation of Suitable Reference Genes for Gene Expression Studies in Callerya speciosa (Champ. ex Benth.) Schot under Different Experimental Conditions
Abstract:The accuracy of reverse transcription quantitative real-time PCR (RT-qPCR) is strongly depended on the stability of reference gene. Callerya speciosa, as a traditionally Chinese medicine, has a long cultivation history in south China. It is essential to select the suitable reference gene to obtain reliable RT-qPCR results when gene expression changes were evaluated. However, suitable reference genes in C. speciosa have not yet been investigated for accurate gene expression quanti cation under different experim… Show more
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