Selection and Validation of miRNA Reference Genes by Quantitative Real-Time PCR Analysis in Paeonia suffruticosa

Zhang, Chenjie; Song, Chengwei; Chen, Linfeng; Ma, Haixiang; Zhang, Yabing; Guo, Da‐Long; Guo, Lili; Hou, Xiaogai

doi:10.3390/horticulturae9020148

Cited by 3 publications

(1 citation statement)

References 60 publications

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“…Therefore, it is also of great significance to explore miRNA related to tree peony flowering so as to realize the regulation of tree peony flowering. However, there was only one report on reference miRNA in tree peony for different bud development processes, flower development processes, and different tissues [ 34 ], but the screening of reference miRNA among different early- and late-flowering tree peony varieties has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Screening of Reference miRNA of Different Early- and Late-Flowering Tree Peony Varieties

Shen

Wang²,

et al. 2023

Plants

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miRNA plays an important role in plant growth and development and in response to various stresses. Quantitative real-time PCR (qRT-PCR) technology is often used to detect the expression level of miRNAs and genes by comparing with reference genes. In order to screen out the optimal reference miRNAs in different tree peony varieties, the petals of 42 different early- and late-flowering tree peony varieties were used as experimental materials, and geNorm, NormFinder, Bestkeeper, and RefFinder software were used to evaluate the stability of 16 candidate reference miRNAs. The results showed that the average Ct values of all candidate reference miRNAs were between 15.34 ± 0.29 and 32.64 ± 0.38. The optimal number of reference miRNAs was four, which were PsPC-5p-19095, PsPC-3p-51259, PsmiR159a, and PsPC-3p-6660 in geNorm. The stability of PsPC-3p-6660 was the highest in the analysis results of NormFinder software. Among the analysis results of Bestkeeper software, PsMIR319-p5 has the highest stability. Among the results of comprehensive evaluation and analysis of several software using RefFinder, the candidate reference miRNA with the highest stability was PsPC-3p-6660. When PsPC-3p-6660 was used as the reference miRNA, the expression of PomiR171 and PomiR414 in response to different flowering times of tree peony was relatively stable in 42 tree peony varieties, indicating that PsPC-3p-6660 was stable and reliable. The results of this study provide a reference miRNA for studying the expression changes of miRNA in different tree peony varieties and further exploring the regulatory mechanism of miRNA in different peony varieties.

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Section: Introductionmentioning

confidence: 99%

Screening of Reference miRNA of Different Early- and Late-Flowering Tree Peony Varieties

Shen

Wang²,

et al. 2023

Plants

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PomiR396g-5p/PoACO1 module regulates the response of tree peony to drought stress through reactive oxygen species pathway

Wang,

Li,

et al. 2024

Industrial Crops and Products

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Identification of Floral Volatile Components and Expression Analysis of Controlling Gene in Paeonia ostii ‘Fengdan’ under Different Cultivation Conditions

Zhang

Niu

et al. 2023

Plants

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In order to explore the release rule of floral volatile substances and the diurnal variation of different flower development stages of Paeonia ostii ‘Fengdan’ in potted and ground-planted conditions, dynamic headspace adsorption combined with gas chromatography-mass spectrometry(GC-MS) was used to analyze the dynamic changes in floral volatile components and contents. Quantitative real-time PCR (qRT-PCR) was used to analyze changes in flower fragrance-regulating genes PsPAL, PsTPSs, and PsbHLH at different flower development stages and a daily change process at the full-blooming stage. The results show that there were differences in aroma components and contents of Paeonia ostii ‘Fengdan’ at different flower development stages and different time quantum of every day. There were 25 and 28 aroma components identified in 7 flower development stages of tree peonies planted in pots and in the field, respectively, and 23 and 22 aroma components identified at different time quantum of the day, of which the largest and highest content was alkanes. The main characteristic aroma substances were (E)-β-ocimene, 1,3,5-trimethoxybenzene, 2,4-di-tert-butylphenol, methyl jasmonate, nerol, and cinnamyl alcohol; released amounts of the abovementioned substances varied depending on the development stage and the time of the day. The expression of flower fragrance-controlling genes (PsPAL, PsTPSs, and PsbHLH) in tree peonies varied greatly in different conditions. The results of this study provide a valuable resource to investigate floral fragrance formation in tree peonies.

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Selection and Validation of miRNA Reference Genes by Quantitative Real-Time PCR Analysis in Paeonia suffruticosa

Cited by 3 publications

References 60 publications

Screening of Reference miRNA of Different Early- and Late-Flowering Tree Peony Varieties

Screening of Reference miRNA of Different Early- and Late-Flowering Tree Peony Varieties

PomiR396g-5p/PoACO1 module regulates the response of tree peony to drought stress through reactive oxygen species pathway

Identification of Floral Volatile Components and Expression Analysis of Controlling Gene in Paeonia ostii ‘Fengdan’ under Different Cultivation Conditions

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