Selection and Transplantation of Hematopoietic Stem and Progenitor Cells

Auditore‐Hargreaves, Karen; Heimfeld, Shelly; Berenson, Ronald J.

doi:10.1021/bc00028a002

Cited by 10 publications

(3 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More importantly, our procedure could be repeated easily and carried out semicontinuously, allowing large numbers of cells to be sorted in a much more optimal manner. Also, our technology does not utilize any enzymes, high-gradient magnetic fields, antibodies, or magnetic particles that may cause undesirable activation and/or inhibition of the cells or immunological reactions in the transplant recipient (Auditore-Hargreaves et al, 1994;Thomas et al, 1999). In addition, the difficulty of removing the target cells from the solid phase is completely eliminated with our technology.…”

Section: Discussionmentioning

confidence: 99%

Cell separation mediated by differential rolling adhesion

Greenberg

Hammer

2001

Biotech & Bioengineering

View full text Add to dashboard Cite

Recently, we showed a correlation between the maturity of hematopoietic stem and progenitor cells during development and rolling efficiency on selectins. These findings motivated us to explore a novel separation that exploits differences in selectin-mediated rolling adhesion between populations of cells. We extend the use of a previously developed cell-free system to study the separation of populations of sialyl Lewis x (sLe(x))-coated microspheres designed to roll with different average velocities on L-selectin chimeric substrates under well-defined flow. Results show that a separation that exploits differences in average rolling velocities between cell or microsphere populations is attainable. Excellent recovery and purity values for the slower rolling, or more desirable, populations are obtained and can be estimated from rolling velocity measurements. We also assess the feasibility of a selectin-mediated separation of adult bone marrow cell populations using previously obtained rolling velocity and rolling flux data for CD34+ and CD34- adult bone marrow cells on L-selectin substrates. We believe that a cell separation mediated by differential rolling adhesion can be used to enrich populations of hematopoietic stem and progenitor cells from an adult bone marrow cell preparation and that this method possesses several major advantages over existing antibody-mediated cell-affinity chromatography technologies.

show abstract

Section: Discussionmentioning

confidence: 99%

Cell separation mediated by differential rolling adhesion

Greenberg

Hammer

2001

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…Some of the most interesting developments are in the fields of microbiology and biomedicine where magnetic particles are used to target, label, manipulate and separate biomaterials such as cells, enzymes, antigens and DNA [1][2][3]. Current clinical cell/ biomolecule sorting devices are based on immunoaffinity columns or on high-gradient magnetic separation (HGMS) columns utilizing either micrometer-sized polymeric beads doped with magnetite, or nanometer-size iron dextran colloids, conjugated to targeting antibodies [4,5]. For HGMS, the two most important design parameters are (a) the magnetic moment of a magnetic label, and (b) the generation of a high magnetic field gradient [6].…”

Section: Introductionmentioning

confidence: 99%

Design and synthesis of plasmonic magnetic nanoparticles

Lim

Tilton

Eggeman

et al. 2007

Journal of Magnetism and Magnetic Materials

View full text Add to dashboard Cite

“…Because of these limitations, less expensive but cruder bulk cell separation methods have been developed. An example of an immunoaffinity technique used for bulk cell separation is an immunoaffinity column, CEPRATE, developed by CellPro (Bothell, WA) (1). This system, by its very nature, is based on a batch process, but high throughput (10 6 cells/s) can be obtained.…”

mentioning

confidence: 99%

Continuous, flow-through immunomagnetic cell sorting in a quadrupole field

et al. 1998

View full text Add to dashboard Cite

A flow-through quadrupole magnetic cell separator has been designed, built, and evaluated by using a cell model system of human peripheral T lymphocytes (CD4 ϩ , CD8 ϩ , and CD45 ϩ cells). The immunomagnetic labeling was accomplished by using a sandwich of mouse anti-human monoclonal antibody conjugated to fluorescein isothiocyanate and rat anti-mouse polyclonal antibody conjugated to a colloidal magnetic nanoparticle. The feed and sorted fractions were analyzed by FACScan flow cytometry. The magnetically labeled cells were separated from nonlabeled ones in a flow-through cylindrical column within a quadrupole field, which exerted a radial, outward force on the magnetic cells. The flow rate of the cell samples was 0.1-0.75 ml/min, and the flow rate of sheath fluid was 1.5-33.3 times that of the sample flow rate. The maximum shear stress exerted on the cell was less than 1 dyne/cm 2 , which was well below the level that would threaten cell integrity and membrane disruption. The maximum magnetic field was 0.765 T at the channel wall, and the gradient was 0.174 T/mm. The highest purity of selected cells was 99.6% (CD8 cells, initial purity of 26%), and the highest recovery of selected cells was 79% (CD4 cells, initial purity of 20%). The maximum throughput of the quadrupole magnetic cell separator was 7,040 cells/s (CD45 cells, initial purity of 5%). Theoretical calculations showed that the throughput can be increased to 10 6 cells/s by a scale-up of the current prototype. Cytometry 33: 469-475, 1998.

show abstract

Selection and Transplantation of Hematopoietic Stem and Progenitor Cells

Cited by 10 publications

References 97 publications

Cell separation mediated by differential rolling adhesion

Cell separation mediated by differential rolling adhesion

Design and synthesis of plasmonic magnetic nanoparticles

Continuous, flow-through immunomagnetic cell sorting in a quadrupole field

Contact Info

Product

Resources

About