Selection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubers

Abstract

Indoleacetic acid (IAA) producing Pseudomonas isolates from the rhizosphere of maize (Q4 and Q20), alfalfa (Q1 and Q16) and wild red clover (B25) were selected for the investigation of their effect on the biological vitality of the potato seed tubers. The production of IAA ranged from 4.09 to 15.9 µ gmL- 1 after 24h of cultivation and 4.08 to 26.4 µ gmL-1 after 48h of cultivation. The molecular comparison by RAPD analysis also was done. RAPD patterns of selected Pseudomonas spp. isolates obtained by BC318, AF14, SPH 1 and AP 10 primers demonstrated the suitability of RAPD method in distinguishing a high variability among the four isolates (44 to 68%). The effect on the biological viability of potato (industrially important variety Pirol) was observed during the seven weeks of sprouting at the temperature of 18-20°C. Potato tubers treated by the selected isolates formed slightly lower number of sprouts, but statistically higher mean length - up to 129.9% higher than the control. The mean sprouting capacity was 64.5% higher than a control. Obtained results suggested positive effects of selected IAA producing Pseudomonas isolates on the length of potato tubers and sprouting capacity as the parameters which define biological viability. [Projekat Ministarstva nauke Republike Srbije, br. III46007

Phytohormone-Producing Rhizobacteria and Their Role in Plant Growth

“…Increased the length of potato tubers and sprouting capacity [39] Pseudomonas fluorescens increased the length of coleoptiles of avena [40] Pseudomonas fluorescens and Bacillus subtilis increase root length, shoot length, root and shoot fresh and dry weight, on bacterial inoculated onion seeds [41] Klebsiella strains significantly increased root and shoot length of inoculated wheat seedlings [42] Bacillus megaterium, Lactobacillus casei, Bacillus subtilis, Bacillus cereus, and Lactobacillus acidophilus, increased growth and yield of wheat and maize [43] Enterobacter spp. increased plant biomass and enhanced phytoextraction of metals (Ni, Zn, and Cr) [44] Gluconacetobacter azotocaptans DS1, Pseudomonas putida CQ179, and Azospirillum lipoferum N7 increased root and shoot weight of corn when compared to uninoculated plants [45] Microbacterium sp., Mycobacterium sp., Bacillus sp., and Rhizobium sp., Sphingomonas sp., Rhodococcus sp., Cellulomonas sp., Pseudomonas sp., and Micrococcus luteus treatment of kidney bean cuttings with bacterial culture liquid promoted formation of a "root brush" with a location height 7.4to 13.4-fold greater than the one in the control samples [46] Pontibacter niistensis a significant increase in the growth of cowpea [47] Cytokinins Bacillus megaterium growth promotion and root developmental responses in Arabidopsis thaliana and Proteus vulgaris seedlings [48] Paenibacillus pofymyxa promoted the growth of plants [37] Rhizobium leguminosarum promoted early seedling root growth of the nonlegumes such as canola (Brassica campestris cv. Tobin, Brassica napus cv.…”