Selection and Methionine Accumulation in the Fat Body Protein 2 Gene (FBP2), a Duplicate of the Drosophila Alcohol Dehydrogenase (ADH) Gene

Meghlaoui, Gilles K.; Veuille, Michel

doi:10.1007/pl00006118

Cited by 16 publications

(6 citation statements)

References 34 publications

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“…Although some protein spots were no longer differential after 4 h, a few new differential spots were observed. A 15-fold increase was observed for fat body protein 2, an alcohol dehydrogenase similar to the one identified in the 25-min experiment (41). Another differential protein spot contains peroxiredoxin, which may play a role in eliminating peroxides generated during metabolism.…”

Section: Resultssupporting

confidence: 52%

A proteomic approach for the analysis of instantly released wound and immune proteins in Drosophila melanogaster hemolymph

Vierstraete

Verleyen

Baggerman

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

138

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Insects respond to microbial infection by the rapid and transient expression of several genes encoding antibacterial peptides. In this paper we describe a powerful technique, two-dimensional difference gel electrophoresis, that, when combined with mass spectrometry, can be used to study the immune response of Drosophila melanogaster at the protein level. By comparatively analyzing the hemolymph proteome of 2,000 third-instar Drosophila larvae, we identified 10 differential proteins that appear in the fruit fly hemolymph very early after an immune-challenge with lipopolysaccharides. These proteins can be assigned to the immune response, because they are not induced after sterile injury. Reduction of intergel variability or quantification problems related to conventional two-dimensional electrophoresis and improvement of image analysis were achieved by the use of two fluorescent dyes to label the two different protein samples. Some of the immune-induced proteins, such as thioestercontaining protein 2, can be assigned to specific aspects of the immune response; others were already reported as being involved in stress response. An immune-induced protein (CG18594) is homologous to a mammalian serine protease inhibitor that mediates the mitogen-activated protein kinase and the NF-B signaling pathways. In addition, a number of proteins that had not been associated with the immune response before were isolated and identified, and some of these were still present in the hemolymph 4 h after injury. Determining the function of all of these immune-induced proteins represents an exciting challenge for increasing our knowledge of insect immunity.immune response proteins ͉ two-dimensional difference gel electrophoresis ͉ stress response ͉ secretome ͉ innate immunity

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Section: Resultssupporting

confidence: 52%

A proteomic approach for the analysis of instantly released wound and immune proteins in Drosophila melanogaster hemolymph

Vierstraete

Verleyen

Baggerman

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

138

View full text Add to dashboard Cite

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“…Early studies showed this gene to have been subject to recurrent selective pressure for methionine accumulation over a long evolutionary time (Rat et al . ; Meghlaoui & Veuille ). A survey of ten chromosomes from Ivory Coast at Fbp 2 (Benassi et al .…”

Section: Discussionmentioning

confidence: 99%

“…The putative function of Fbp2 is to supply larval instars with methionine during the first development stages. Early studies showed this gene to have been subject to recurrent selective pressure for methionine accumulation over a long evolutionary time (Rat et al 1991;Meghlaoui & Veuille 1997). A survey of ten chromosomes from Ivory Coast at Fbp2 (Benassi et al 1999) revealed a selective sweep encompassing a 1.3-kb region.…”

Section: Evidence For Positive Selection At Fbp2mentioning

confidence: 99%

Geographical delimitation of a partial selective sweep in African Drosophila melanogaster

et al. 2012

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Positive selection leaves characteristic footprints on DNA variation but detecting such patterns is challenging as the age, the intensity and the mode of selection as well as demography and evolutionary parameters (mutation and recombination rates) all play roles and these are difficult to disentangle. We recorded nucleotide variation in a sample of isogenic chromosomes from a western African population of Drosophila melanogaster at a locus (Fbp2) for which a partial selective sweep had previously been reported. We compared this locus to four other genes from the same chromosomes and from a European and an East African population. Then, we assessed Fbp2 variation in a sample of 370 chromosomes covering a comprehensive geographic sampling of 16 African localities. The signature of selection was tested while accounting for the demographic history of the populations. We found a significant signal of selection in two West African localities including Ivory Coast. Variation at Fpb2 would thus represent a case of an ongoing selective sweep in the range of this species. A weaker, nonsignificant, signal of selection was, however, apparent in some other populations, thus leaving open several possibilities: (i) the selective sweep originated in Ivory Coast and has spread to the rest of the continent; (ii) several African populations report the signature of a selective event having occurred in an ancestral population; (iii) this genome region is subject to independent selective events in African populations; and (iv) A neutral scenario with population subdivision and local bottleneck cannot be fully excluded to explain the molecular patterns observed in some populations.

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“…We acknowledge that the s Mel- L. heterotoma (strain Lh14) interaction, albeit leading to wasp death, seldom results in fly surviva(Xie et al, 2014; Paredes et al, 2016; Jones and Hurst, 2020b). Given its possible function as a sulfur-storage protein (Meghlaoui and Veuille, 1997), increased expression of Fbp2 might reflect a greater availability of nutrients for the fly, which might enhance its tolerance to insults from the wasp. Similarly, based on its association to “response to stress”, increased expression of TotC may contribute to increasing the fly’s tolerance to the wasp parasitism.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic response during wasp parasitism in theDrosophila-Spiroplasmainteraction

Higareda‐Alvear¹,

Mateos²,

Cortez³

et al. 2020

Preprint

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Several facultative bacterial symbionts of insects protect their hosts against natural enemies. Spiroplasma poulsonii strain sMel, a male-killing heritable symbiont of Drosophila melanogaster, confers protection against some species of parasitic wasps. Several lines of evidence suggest that Spiroplasma-encoded ribosome inactivating proteins (RIPs) are involved in the protection mechanism, but the potential contribution of the fly-encoded functions has not been deeply explored. Here we used RNA-seq to evaluate the response of D. melanogaster to infection by Spiroplasma and parasitism by the Spiroplasma-susceptible wasp Leptopilina heterotoma, and the Spiroplasma-resistant wasp Ganaspis hookeri. In the absence of Spiroplasma infection, we found evidence of Drosophila immune activation by G. hookeri, but not by L. heterotoma, which in turn negatively influenced functions associated with male gonad development. As expected for a symbiont that kills males, we detected extensive downregulation in the Spiroplasma-infected treatments of genes known to have male-biased expression. We detected very few genes whose expression was influenced by the Spiroplasma-L. heterotoma interaction, and they do not appear to be related to immune response. For most of them, parasitism by L. heterotoma (in the absence of Spiroplasma) caused an expression change that was at least partly reversed when Spiroplasma was also present. It is unclear whether such genes are involved in the Spiroplasma-mediated mechanism that leads to wasp death or fly rescue. Nonetheless, the expression pattern of some of these genes, which reportedly undergo expression shifts during the larva-to-pupa transition, is suggestive of an influence of Spiroplasma on the development time of L. heterotoma-parasitized flies. In addition, we used the RNAseq data and quantitative (q)PCR to evaluate the transcript levels of the Spiroplasma-encoded RIP genes. One of the five RIP genes (RIP2) was consistently highly expressed independently of wasp parasitism, in two substrains of sMel. Finally, the RNAseq data revealed evidence consistent with RIP-induced damage in the ribosomal (r)RNA of the Spiroplasma-susceptible, but not the Spiroplasma-resistant, wasp. We conclude that immune priming is unlikely to contribute to the Spiroplasma-mediated protection against wasps, and that the mechanism by which G. hookeri resists/tolerates Spiroplasma does not involve inhibition of RIP transcription.

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Selection and Methionine Accumulation in the Fat Body Protein 2 Gene (FBP2), a Duplicate of the Drosophila Alcohol Dehydrogenase (ADH) Gene

Cited by 16 publications

References 34 publications

A proteomic approach for the analysis of instantly released wound and immune proteins in Drosophila melanogaster hemolymph

A proteomic approach for the analysis of instantly released wound and immune proteins in Drosophila melanogaster hemolymph

Geographical delimitation of a partial selective sweep in African Drosophila melanogaster

Transcriptomic response during wasp parasitism in theDrosophila-Spiroplasmainteraction

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