Selection and mass spectrometry characterization of peptides targeting semiconductor surfaces

Abstract

We report on elaboration of 12-mer peptides that reveal specific recognition for the following semiconductor (SC) surfaces: GaAs(100), InAs(100), GaN(0001), ZnSe(100), ZnTe(100), GaAs(111)A, GaSb(100), CdSe(100). A M13 bacteriophage library was used to screen 10(9) different 12-mer peptides against these substrates to finally isolate, in maximum six amplification cycles, peptides that bind to the target surfaces. The specific peptides for the InAs and ZnSe surfaces were obtained. Contrary, for the other SC surfaces several peptides with high affinities have been isolated. Aiming for a better specificity, when the phage display has been conducted through six cycles, the screening procedure got dominated by a phage present in the M13 bacteriophage library and the SVSVGMKPSPRP peptide has been selected for different SCs. The high amplification potential of this phage has been observed previously with different targets. Thus, precaution should be undertaken in defining adhesion peptides with the phage display technique and real affinity of the obtained biolinkers should be studied with other methods. We employed mass spectrometry (MALDI-TOF/TOF) to demonstrate the preferential attachment (or not) of the SVSVGMKPSPRP peptide to the different SC surfaces. This allows us to define a realistic selection of the expressed peptides presenting affinity for the studied eight SC surfaces. We demonstrate that with increasing the dielectric constants of the employed solvents, adhesion of the SVSVGMKPSPRP peptide onto GaN(0001) is hindered.

Peptides for the Biofunctionalization of Silicon for Use in Optical Sensing with Porous Silicon Microcavities

“…The SVSVGMKPSPRP (P1) peptide was expressed by most of the selected phages (Table 1) on the three Si types, and it has been also found in the phage library selected on other SCs for different inorganic and organic targets 16, 29–33. Some authors consider P1 the researched peptide, whereas others discard it following discussion with the library producer, stating that there is a super‐infectious phage clone in the library showing high amplification potential 32, 33.…”

“…Peptides targeting inorganic materials have been used for the assembly of inorganic particles and the formation of selected materials 13–15. We have previously reported the elaboration of adhesion peptides via phage display technology for use with other semiconductors16 and their use for the selective functionalization of GaN and InP patterned structures 17, 18. This new functionalization method provides selective functionalization of SCs (also within a multi‐component material) via surface modification with short adhesion peptides that can specifically recognize materials, thus eliminating the need for masks 19, 20.…”

Biotemplating rod-like viruses for the synthesis of copper nanorods and nanowires

“…fd and M13 differ by one amino acid per CP, which results in a net 30% more negative charge in fd [17,18]. The M13 phage is a widely-used cloning system as a phage display for expression of small peptides [19] used to identify amino acid sequences that are specific towards metals, metal oxides [20], and semiconductor surfaces [21]. …”

Bottom-up synthesis of protein-based nanomaterials from engineered β-solenoid proteins

“…These monomers fibrillize in a cell-free environment, do not require harsh conditions or accessory proteins to assemble, and are extremely stable, highlighting their potential for engineering into 2D and 3D scaffolds that can be used to create functional nanomaterials. The attachment of material-specific binding peptides [61][62][63][64][65][66] to the N-or C-terminus of BSPs may provide a simple route to template a wide variety of materials.…”