Selection and identification of xylose-fermenting yeast strains for ethanol production from lignocellulosic biomass

Araújo, Juliana Alves; Abreu-Lima, Thiago Lucas de; Carreiro, Solange Cristina

doi:10.5380/bceppa.v36i1.59557

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…Natural xylose-fermenting yeast strains such as Candida shehatae, Kluyveromyces marxianus, Pachysolen tannophilus, and Pichia stipitis have been the most thoroughly studied and are the most efficient xylose fermenters (Antunes et al 2013 , Laluce et al 2012 ). Several other yeast species that are also capable of metabolizing xylose include Aerobasidium pullulans, Candida tropicalis, Candida oleophila, Hanseniaspora guilliermondii, Issatchenkia terricola, Metschnikowia koreensis, Pichia guilliermondii, and Rhodotorula mucilagi nosa (Araújo et al 2018 , Martins et al 2018 ; Ruchala and Sibirny 2021 ). They are most recognized for being able to convert xylose to ethanol.…”

Section: Resultsmentioning

confidence: 99%

Isolation of xylose-utilizing yeasts from oil palm waste for xylitol and ethanol production

Kusumawati,

Sumarlan,

Zubaidah

et al. 2023

Bioresour. Bioprocess.

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The energy crisis triggers the use of energy sources that are renewable, such as biomass made from lignocellulosic materials, to produce various chemical compounds for food ingredients and biofuel. The efficient conversion of lignocellulosic biomass into products with added value involves the activity of microorganisms, such as yeasts. For the conversion, microorganisms must be able to use various sugars in lignocellulosic biomass, including pentose sugars, especially xylose. This study aims to isolate xylose-utilizing yeasts and analyze their fermentation activity to produce xylitol and ethanol, as well as their ability to grow in liquid hydrolysate produced from pretreated lignocellulosic biomass. Nineteen yeast isolates could grow on solid and liquid media using solely xylose as a carbon source. All isolates can grow in a xylose medium with incubation at 30 °C, 37 °C, 42 °C, and 45 °C. Six isolates, namely SLI (1), SL3, SL6, SL7, R5, and OPT4B, were chosen based on their considerable growth and high xylose consumption rate in a medium with 50 g/L xylose with incubation at 30 °C for 48 h. Four isolates tested, namely SLI (1), SL6, SL7, and R5, can produce xylitol in media containing xylose carbon sources. The concentration of xylitol produced was determined using high-pressure liquid chromatography (HPLC), and the results ranged from 5.0 to 6.0 g/L. Five isolates tested, namely SLI (1), SL6, SL3, R5, and OPT4B, can produce ethanol. The ethanol content produced was determined using gas chromatography (GC), with concentrations ranging from 0.85 to 1.34 g/L. Three isolates, namely SL1(1), R5, and SL6, were able to produce xylitol and ethanol from xylose as carbon sources and were also able to grow on liquid hydrolyzate from pretreated oil palm trunk waste with the subcritical water method. The three isolates were further analyzed using the 18S rDNA sequence to identify the species and confirm their phylogenetic position. Identification based on DNA sequence analysis revealed that isolates SL1(1) and R5 were Pichia kudriavzevii, while isolate SL6 was Candida xylopsoci. The yeast strains isolated from this study could potentially be used for the bioconversion process of lignocellulosic biomass waste to produce value-added derivative products. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

Isolation of xylose-utilizing yeasts from oil palm waste for xylitol and ethanol production

Kusumawati,

Sumarlan,

Zubaidah

et al. 2023

Bioresour. Bioprocess.

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“…Only 13 isolates were able to produce gas in Durham tubes (data not shown) on both pentose sugars. Araújo et al [ 31 ] screened xylose-fermenting ability among 205 yeast isolates obtained from fruit pulp and plants of Cerrado. They found that only 3 isolates were able to ferment D-xylose in test tubes.…”

Section: Discussionmentioning

confidence: 99%

The Improvement of Bioethanol Production by Pentose-Fermenting Yeasts Isolated from Herbal Preparations, the Gut of Dung Beetles, and Marula Wine

Moremi

Rensburg

Grange

2020

International Journal of Microbiology

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Efficient conversion of pentose sugars to ethanol is important for an economically viable lignocellulosic bioethanol process. Ten yeasts fermenting both D-xylose and L-arabinose were subjected to an adaptation process with L-arabinose as carbon source in a medium containing acetic acid. Four Meyerozyma caribbica-adapted strains were able to ferment L-arabinose to ethanol in the presence of 3 g/L acetic acid at 35°C. Meyerozyma caribbica Mu 2.2f fermented L-arabinose to produce 3.0 g/L ethanol compared to the parental strain with 1.0 g/L ethanol in the absence of acetic acid. The adapted M. caribbica Mu 2.2f strain produced 3.6 and 0.8 g/L ethanol on L-arabinose and D-xylose, respectively, in the presence of acetic acid while the parental strain failed to grow. In a bioreactor, the adapted M. caribbica Mu 2.2f strain produced 5.7 g/L ethanol in the presence of 3 g/L acetic acid with an ethanol yield and productivity of 0.338 g/g and 0.158 g/L/h, respectively, at a KLa value of 3.3 h−1. The adapted strain produced 26.7 g/L L-arabitol with a yield of 0.900 g/g at a KLa value of 4.9 h−1.

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Selection and identification of xylose-fermenting yeast strains for ethanol production from lignocellulosic biomass

Cited by 2 publications

References 6 publications

Isolation of xylose-utilizing yeasts from oil palm waste for xylitol and ethanol production

Isolation of xylose-utilizing yeasts from oil palm waste for xylitol and ethanol production

The Improvement of Bioethanol Production by Pentose-Fermenting Yeasts Isolated from Herbal Preparations, the Gut of Dung Beetles, and Marula Wine

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