Selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities

Toledo, F.L.; Calvo, C.; Rodelas, B.; González‐López, Jesús

doi:10.1016/j.syapm.2005.09.003

Cited by 120 publications

(80 citation statements)

References 32 publications

(26 reference statements)

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“…Bacteria play an important role in environmental applications such as the remediation of nitrates and organic contaminants in groundwater (Toledo et al, 2006), the bioleaching of ores (e.g., Eisele and Gabby, 2014), managed aquifer recharge (Cockett and Pidlisecky, 2014), and the natural attenuation of leachates from landfills (Naudet et al, 2004). In addition, there is a growing interest in understanding the role of indigenous anaerobic bacteria in biodegraded oil fields (Grabowski et al, 2005) as well as in situ bioaugmentation and biostimulation of contaminated groundwater (Ginn et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Quadrature conductivity: A quantitative indicator of bacterial abundance in porous media

et al. 2014

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The abundance and growth stages of bacteria in subsurface porous media affect the concentrations and distributions of charged species within the solid-solution interfaces. Therefore, spectral induced polarization (SIP) measurements can be used to monitor changes in bacterial biomass and growth stage. Our goal was to gain a better understanding of the SIP response of bacteria present in a porous material. Bacterial cell surfaces possess an electric double layer and therefore become polarized in an electric field. We performed SIP measurements over the frequency range of 0.1-1 kHz on cell suspensions alone and cell suspensions mixed with sand at four pore water conductivities. We used Zymomonas mobilis at four different cell densities (including the background). The quadrature conductivity spectra exhibited two peaks, one around 0.05-0.10 Hz and the other around 1-10 Hz. Because SIP measurements on bacterial suspensions are typically made at frequencies greater than 1 Hz, these peaks have not been previously reported. In the bacterial suspensions in growth medium, the quadrature conductivity at peak I was linearly proportional to the density of the bacteria. For the case of the suspensions mixed with sands, we observed that peak II presented a smaller increase in the quadrature conductivity with the cell density. A comparison of the experiments with and without sand grains illustrated the effect of the porous medium on the overall quadrature conductivity response (decrease in the amplitude and shift of the peaks to the lower frequencies). Our results indicate that for a given porous medium, time-lapse SIP has potential for monitoring changes in bacterial abundance within porous media.

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Section: Introductionmentioning

confidence: 99%

Quadrature conductivity: A quantitative indicator of bacterial abundance in porous media

et al. 2014

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“…Whyte, et al, (1997) conducted similar experiments, studying biodegradation of naphthalene by bacteria isolated from oilcontaminated soils. Toledo et al, (2006) studied bacterial strains isolated from waste crude oil and their capacity for growth with naphthalene, phenanthrene, fluoranthene and pyrine as sole carbon sources. Our study differs from these, however, in that we studied growth rate of bacteria only in the presence of aromatic hydrocarbons.…”

Section: Biodegradationmentioning

confidence: 99%

Role of Microorganisms for the Sustainable Use of Soil Pollution Abutment in Agriculture Lands

Verma¹,

Devi²,

Sinha³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…According to Toledo et al [32], the relative proportions of these fractions vary between oil types, and the susceptibility of specific oil to microbial degradation can be predicted from this composition. Moreover, waste lubricating oils may have highly variable compositions according to the extent of combustion process during its functioning, so the biodegradation analysis was done by comparing the content of recoverable total petroleum hydrocarbons (TPH) from influent and effluent oil samples.…”

Section: Characterization Of Waste Lubricating Oil Biodegradationmentioning

confidence: 99%

Waste lubricating oil removal in a batch reactor by mixed bacterial consortium: a kinetic study

Bhattacharya

Guchhait

Biswas

et al. 2015

Bioprocess Biosyst Eng

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The growth kinetics and biodegradation of two waste lubricating oil samples including waste engine oil (WEO) and waste transformer oil (WTO) were studied using pure isolates and mixed culture of Ochrobactrum sp. C1 and Bacillus sp. K1. The mixed culture significantly influenced degradation efficiency of the pure isolates through bioaugmentation process. In particular, the mixed culture was capable of growing on various n-alkanes and polycyclic aromatic hydrocarbons and was able to tolerate unusually high concentrations of waste lubricants (WEO-86.0 g/L and WTO-81.5 g/L). The initial concentration of waste lubricating oils has been varied in the range of 1-10 % (v/v). Under this experimental range, the bacterial growth has been observed to follow Haldane-type kinetics characterizing the presence of substrate inhibition. Haldane model was used to fit the exponential growth data and the following kinetic parameters were obtained: μ max = 0.078 h(-1), K S = 23.101 g/L, K i = 43.844 g/L for WEO; and μ max = 0.044 h(-1), K S = 10.662 g/L, K i = 58.310 g/L for WTO. The values of intrinsic kinetic parameters, like specific growth rate μ max, half saturation constant, K S, inhibition constant, K i and the maximum substrate concentration, S max and growth yield coefficient Y x/s , have been determined using each model hydrocarbon and their mixture as limiting substrate. Relative changes in the values of the kinetic parameters have been correlated to the number of carbon atoms present in n-alkanes. The metabolites from degradation of model hydrocarbon compounds have been identified by GC-MS to elucidate the possible pathway of waste lubricating oil degradation process.

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Selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities

Cited by 120 publications

References 32 publications

Quadrature conductivity: A quantitative indicator of bacterial abundance in porous media

Quadrature conductivity: A quantitative indicator of bacterial abundance in porous media

Role of Microorganisms for the Sustainable Use of Soil Pollution Abutment in Agriculture Lands

Waste lubricating oil removal in a batch reactor by mixed bacterial consortium: a kinetic study

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