Selection and evaluation of microorganisms for biodegradation of agricultural plastic film

Zhang, Jing; Chen, Jing; Jia, Ruimin; Dun, Zhihen; Wang, Baotong; Hu, Xiaopin; Wang, Yan

doi:10.1007/s13205-018-1329-5

Cited by 13 publications

(16 citation statements)

References 24 publications

(21 reference statements)

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“…SEM analysis of polymer biodegradation by Penicillium simplicissimum showed the formation of cavities and physical structural changes in the polymer (Sowmya et al 2015). Our FTIR findings on PVA biodegradation principal spectral peaks including their common functional groups, were similar to findings obtained in previous studies (Ullah et al 2019;Zhang et al 2018). In a study by Nwogu et al (2012), degradation of plastic using mushrooms was also confirmed via FTIR analyses.…”

Section: Discussionsupporting

confidence: 88%

Biodegradation of poly (vinyl alcohol) by an orychophragmus rhizosphere-associated fungus Penicillium brevicompactum OVR-5, and its proposed PVA biodegradation pathway

Mohamed

Shah

Nazir

et al. 2021

World J Microbiol Biotechnol

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In recent years, the utilisation of endophytes has emerged as a promising biological treatment technology for the degradation of plastic wastes such as biodegradation of synthetic plastics. This study, therefore, aimed to explore and extensively screen endophytic fungi (from selected plants) for efficient in vitro polyvinyl alcohol (PVA) biodegradation. In total, 76 endophytic fungi were isolated and cultivated on a PVA screening agar medium. Among these fungi, 10 isolates showed potential and were subsequently identified based on phenotypical characteristics, ITS ribosomal gene sequences, and phylogenetic analyses. Four strains exhibited a maximum level of PVA-degradation in the liquid medium when cultivated for 10 days at 28 °C and 150 rpm. These strains showed varied PVA removal rates of 81% (Penicillium brevicompactum OVR-5), 67% (Talaromyces verruculosus PRL-2), 52% (P. polonicum BJL-9), and 41% (Aspergillus tubingensis BJR-6) respectively. The most promising PVA biodegradation isolate 'OVR-5', with an optimal pH at 7.0 and optimal temperature at 30 °C, produced lipase, manganese peroxidase, and laccase enzymes. Based on analyses of its metabolic intermediates, as identified with GC-MS, we proposed the potential PVA degradation pathway of OVR-5. Biodegradation results were confirmed through scanning electron microscopy and Fourier transform infrared spectroscopy. This study provides the first report on an endophytic P. brevicompactum strain (associated with Orychophragmus violaceus) that has a great ability for PVA degradation providing more insight on potential fungus-based applications in plastic waste degradation.

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Section: Discussionsupporting

confidence: 88%

Biodegradation of poly (vinyl alcohol) by an orychophragmus rhizosphere-associated fungus Penicillium brevicompactum OVR-5, and its proposed PVA biodegradation pathway

Mohamed

Shah

Nazir

et al. 2021

World J Microbiol Biotechnol

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“…However, the specific complex involved in toluene degradation in PM1 remains unknown (Deeb et al, 2001). The only genome lacking expected POPs belonged to the strain Bacillus amyloliquefaciens HK1, despite experimental evidence of its capability to degrade PVA (Zhang et al, 2018). This result suggested that this isolate likely has proteins involved in PVA degradation that are not currently present in our database.…”

Section: Resultsmentioning

confidence: 99%

HADEG: A Curated Hydrocarbon Aerobic Degradation Enzymes and Genes Database

Rojas-Vargas¹,

Castelán-Sánchez

Pardo-López³

2022

Preprint

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Databases of genes and enzymes involved in natural and synthetic hydrocarbon degradation have been previously reported. However, these databases specialize on only a specific group of hydrocarbons and/or are constructed partly based on enzyme sequences with putative functions indicated by in silico research, with no experimental evidence. Here, we present a curated database of Hydrocarbon Aerobic Degradation Enzymes and Genes (HADEG) containing enzymes and genes involved in alkane, alkene, aromatic, and polymer aerobic degradation and biosurfactant production based solely on experimental evidence. HADEG includes 259 enzymes for petroleum hydrocarbon degradation, 160 for polymer degradation, and 32 for biosurfactant production. This database will help identify and predict hydrocarbon degradation genes/pathways and biosurfactant production in genomes.

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“…Varying CAZyme profiles of intestinal microbes may had different capabilities of carbohydrate metabolism [42]. Comparison of CAZyme genes in five B. amyloliquefaciens strains, including TL106, HK1, EA19, LL3 and XH7 revealed that B. amyloliquefaciens TL106 had more types of CAZymes genes than the other four strains [43][44][45][46]. GH genes were took a large part in the genome of B. amyloliquefaciens, consistent with those of the genome of Aspergillus fumigatus Z5, which showed highly efficient cellulose-degradation capability [47].…”

Section: Discussionmentioning

confidence: 99%

Complete genome sequencing and investigation on the fiber-degrading potential of Bacillus amyloliquefaciens strain TL106 from the tibetan pig

et al. 2022

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Background Cellulolytic microorganisms are considered a key player in the degradation of feed fiber. These microorganisms can be isolated from various resources, such as animal gut, plant surfaces, soil and oceans. A new strain of Bacillus amyloliquefaciens, TL106, was isolated from faeces of a healthy Tibetan pigs. This strain can produce cellulase and shows strong antimicrobial activity in mice. Thus, in this study, to better understand the strain of B. amyloliquefaciens TL106 on degradation of cellulose, the genome of the strain TL106 was completely sequenced and analyzed. In addition, we also explored the cellulose degradation ability of strain TL106 in vitro. Results TL106 was completely sequenced with the third generation high-throughput DNA sequencing. In vitro analysis with enzymatic hydrolysis identified the activity of cellulose degradation. TL106 consisted of one circular chromosome with 3,980,960 bp and one plasmid with 16,916 bp, the genome total length was 3.99 Mb and total of 4,130 genes were predicted. Several genes of cellulases and hemicellulase were blasted in Genbank, including β-glucosidase, endoglucanase, ß-glucanase and xylanase genes. Additionally, the activities of amylase (20.25 U/mL), cellulase (20.86 U/mL), xylanase (39.71 U/mL) and β-glucanase (36.13 U/mL) in the fermentation supernatant of strain TL106 were higher. In the study of degradation characteristics, we found that strain TL106 had a better degradation effect on crude fiber, neutral detergent fiber, acid detergent fiber, starch, arabinoxylan and β-glucan of wheat and highland barley . Conclusions The genome of B. amyloliquefaciens TL106 contained several genes of cellulases and hemicellulases, can produce carbohydrate-active enzymes, amylase, cellulase, xylanase and β-glucanase. The supernatant of fermented had activities of strain TL106. It could degrade the fiber fraction and non-starch polysaccharides (arabinoxylans and β-glucan) of wheat and highland barley. The present study demonstrated that the degradation activity of TL106 to crude fiber which can potentially be applied as a feed additive to potentiate the digestion of plant feed by monogastric animals.

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Selection and evaluation of microorganisms for biodegradation of agricultural plastic film

Cited by 13 publications

References 24 publications

Biodegradation of poly (vinyl alcohol) by an orychophragmus rhizosphere-associated fungus Penicillium brevicompactum OVR-5, and its proposed PVA biodegradation pathway

Biodegradation of poly (vinyl alcohol) by an orychophragmus rhizosphere-associated fungus Penicillium brevicompactum OVR-5, and its proposed PVA biodegradation pathway

HADEG: A Curated Hydrocarbon Aerobic Degradation Enzymes and Genes Database

Complete genome sequencing and investigation on the fiber-degrading potential of Bacillus amyloliquefaciens strain TL106 from the tibetan pig

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