Selection and Characterization of a Peptide Specifically Targeting to Gastric Cancer Cell Line SGC-7901 Using Phage Display

Wang, Han; Li, Rui; Ma, Cun‐Gen; Lu, Shaoying; Zhang, Dan; Guo, Yonge; Li, Chunyan; Wu, Junchen; Wang, Qixuan; Xu, Jinhui; Hu, Yawen; Liu, Yuen; Song, Xigui; Hou, Yingchun

doi:10.1007/s10989-013-9367-7

Cited by 8 publications

(2 citation statements)

References 11 publications

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“…It is expected that, when employed as a tumor targeting agent, peptide could be selectively penetrated in tumor tissue, and then further drug will be released in vivo. However, most of these peptides were discovered by phage display screening or combinatorial peptide libraries with quite long time and high cost. , In contrast, computationally designing homing peptides through virtual docking is promising and challenging . It relies on the crystal structures of novel surface proteins and the precision of the binding site.…”

Section: Introductionmentioning

confidence: 99%

“…However, most of these peptides were discovered by phage display screening or combinatorial peptide libraries with quite long time and high cost. 18,19 In contrast, computationally designing homing peptides through virtual docking is promising and challenging. 20 It relies on the crystal structures of novel surface proteins and the precision of the binding site.…”

Section: ■ Introductionmentioning

confidence: 99%

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De Novo Computational Design for Development of a Peptide Ligand Oriented to VEGFR-3 with High Affinity and Long Circulation

Dong

Wang

et al. 2017

Mol. Pharmaceutics

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The overexpression of VEGFR-3 is correlated with a worse prognosis in lung cancer and has been regarded as a rational target for specific drug delivery. Here, VEGFR-3 homing peptide library was efficiently established by computational design. Strong fluorescent signals of selected peptides were observed in A549 cells, but much weaker in other cells. The positive immunostaining overlapped with VEGFR-3 confirmed high affinity and selectivity of one novel peptide (CP-7). In addition, cell uptake of FITC-CP-7 peptide was significantly blocked by coinjection of excess CP-7 peptide. After labeled with I, the profile of pharmacology and biodistribution could be traced in vivo. TheI-radiolabeled CP-7 peptide conjugates were >85% stable in serum over 4 h and exhibited a specific uptake of 18.04 ± 2.04% ID/g at 0.5 h after injection to high VEGFR-3 expressing A549 tumor mice. More importantly, lower uptake concentration in heart (1.06 ± 0.15% ID/g) after 2 h demonstrated the safety of peptide in vivo. The high uptake in the kidneys revealed that renal clearance was the main route of I-CP-7 peptide elimination from the body. Lower accumulation ofI-CP-7 peptide in VEGFR-3 negative HeLa tumor mice further indicated that CP-7 peptide exhibited a higher tumor-homing efficiency. These studies provided a straightforward analytical access to design and screen bioactive peptide based on protein structure and revealed that CP-7 peptide represented a promising homing peptide of VEGFR-3-positive cancer in vitro and in vivo which could be used as a novel target molecule to achieve efficient drug delivery.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

De Novo Computational Design for Development of a Peptide Ligand Oriented to VEGFR-3 with High Affinity and Long Circulation

Dong

Wang

et al. 2017

Mol. Pharmaceutics

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Phage nanofibers in nanomedicine: Biopanning for early diagnosis, targeted therapy, and proteomics analysis

Cao

et al. 2020

WIREs Nanomed Nanobiotechnol

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Display of a peptide or protein of interest on the filamentous phage (also known as bacteriophage), a biological nanofiber, has opened a new route for disease diagnosis and therapy as well as proteomics. Earlier phage display was widely used in protein–protein or antigen–antibody studies. In recent years, its application in nanomedicine is becoming increasingly popular and encouraging. We aim to review the current status in this research direction. For better understanding, we start with a brief introduction of basic biology and structure of the filamentous phage. We present the principle of phage display and library construction method on the basis of the filamentous phage. We summarize the use of the phage displayed peptide library for selecting peptides with high affinity against cells or tissues. We then review the recent applications of the selected cell or tissue targeting peptides in developing new targeting probes and therapeutics to advance the early diagnosis and targeted therapy of different diseases in nanomedicine. We also discuss the integration of antibody phage display and modern proteomics in discovering new biomarkers or target proteins for disease diagnosis and therapy. Finally, we propose an outlook for further advancing the potential impact of phage display on future nanomedicine. This article is categorized under: Biology‐Inspired Nanomaterials > Protein and Virus‐Based Structures

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Molecular targeted treatment and drug delivery system for gastric cancer

Jiang

Gong

Liao

et al. 2021

J Cancer Res Clin Oncol

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Selection and Characterization of a Peptide Specifically Targeting to Gastric Cancer Cell Line SGC-7901 Using Phage Display

Cited by 8 publications

References 11 publications

De Novo Computational Design for Development of a Peptide Ligand Oriented to VEGFR-3 with High Affinity and Long Circulation

De Novo Computational Design for Development of a Peptide Ligand Oriented to VEGFR-3 with High Affinity and Long Circulation

Phage nanofibers in nanomedicine: Biopanning for early diagnosis, targeted therapy, and proteomics analysis

Molecular targeted treatment and drug delivery system for gastric cancer

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