Selection and Characteristics of Fermented Salted Seafood (jeotgal)-Originated Strains with Excellent S-adenosyl-L-methionine (SAM) Production and Probiotics Efficacy

Abstract

This study is executed to develop probiotics which produce S-adenosyl-L-methionine (SAM), a methyl group donor of the 5-methyltetrahydrofolate methylation reaction within the animal cell. SAM is an essential substance for the synthesis, activation, and metabolism of hormones, neurotransmitters, nucleic acids, phospholipids, and cell membranes of animals. The SAM is also known as a nutritional supplement to improve brain functions of the human. In this study, the SAM-producing strains are identified in 18 types of salted fish, and then, the strains with excellent SAM productions are being identified, with 1 strain in the Enterococcus genus and 9 strains in the Bacillus genus. Strains with a large amount of SAM production include the lactic acid bacteria such as En. faecium and En. durans, En. sanguinicola, as well as various strains in the Bacil-lus genus. The SAM-overproducing strains show antibacterial activities with certain harmful microbes in addition to the weak acid resistances and strong bile resistances, indicating characteristics of probiotics. It is possible that the jeotgal-originated beneficial strains with overproducing SAM can be commercially utilized in order to manufacture SAM enriched foods.

Probiotics Affect One‐Carbon Metabolites and Catecholamines in a Genetic Rat Model of Depression

“…Lactic acid bacteria in fermented Cheonggukjang have been shown to produce SAM in vitro, suggesting that SAM produced through gut bacterial fermentation could be available to the host. Alterations in host C1 metabolism in the probiotic group could be due to the bacteria's own production of SAM (GenomeNet Database Resources) (Supporting Information Table 6).…”

“…Therefore, high serotonin in vehicle‐treated FSL rats could have caused reduced dopamine degradation via SAM‐dependent COMT. Lactobacillus strains produce SAM in vitro and L. helveticus is also able to produce C1 units and metabolize phenylalanine and tyrosine via transamination and dehydrogenation pathways, suggesting that they could have provided BH4 or other intermediate metabolites of phenylalanine and tyrosine that could have influenced dopamine production in extraneuronal tissues.…”

Identification, Classification and Screening for γ-Amino-butyric Acid Production in Lactic Acid Bacteria from Cambodian Fermented Foods

“…While P. pentosaceus and E. faecium can grow in the presence of ≥6.5% NaCl [66,67], salt resistance is strain-dependent in the species E. hermanniensis [68]. As enterococci are found in a variety of different ecological environments including surface and waste waters, their association with fermented seafood [64,69,70] can be explained.…”